Polyethylene glycol-chitosan dual-network hydrogel as well as preparation method and application thereof

Abstract

The invention relates to polyethylene glycol-chitosan double-network hydrogel as well as a preparation method and application thereof, and belongs to the technical field of novel medical materials. The method comprises the following steps: enabling a KGN solution activated by EDCI / NHS to react with Tetra-PEG-NH2 to obtain KGN-Tetra-PEG-NH2, and preparing the KGN-Tetra-PEG-NH2 into a first solution; reacting the chitosan solution with Tetra-PEG-NHS (Polyethylene Glycol-Hydroxysuccinimide) for 10-300 seconds according to the molar ratio of amino to NHS of (0.01-0.25): 1, so as to obtain a second solution; mixing the first solution and the second solution according to the molar ratio of amino to NHS of (0.8-1.2): 1, standing to form gel, and activating to obtain the polyethylene glycol-chitosan double-network hydrogel. The hydrogel provided by the invention has the advantages of high mechanical strength, good cartilage induced differentiation microenvironment, good cartilage repair effect and the like.

Description

Technical field [0001] The present invention is a medical technology field, especially involving a polyethylene-shell polysaccharide dual-network hydrogel and its preparation methods and applications. Background technique [0002] Sports -related trauma or degenerative changes can cause cartilage injuries. Due to the characteristics of cartilage without blood vessels, nervous, no lymphatic vessels, low metabolic rates and low cell density, it is difficult to self -repair after the injury. At present, traditional clinical treatment methods mainly include arthroscopy cleaning, cartilage grinding molding, bone marrow stimulus technology, autologous or similar album cartilage transplantation, autologous cartilage cell transplantation, metal or silicone rubber transplantation, etc. , Limitation and deficiencies such as the complications of the supply body, the failure of the transplants, the dewlating of seed cells, and the loosening of alternatives. Fortunately, the organizational en...

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Problems solved by technology

[0005] Chinese patent application CN112646202A discloses a functionalized double-network hydrogel and its preparation method. The double-network hydrogel in this patent application is formed by interpenetrating a chitosan cross-linked network and a four-armed polyethylene glycol cross-linked network. , the chitosan cross-linked network is formed by cross-linking chitosan coupled with KGN; the four-arm polyethylene glycol cross-linked network is formed by chemical reaction of the first precursor and the second precursor, The first precursor is Tetra-PEG-NH 2 , the second precursor is Tetra-PEG-NHS, but the chitosan cross-linked network and the four-arm polyethylene glycol cross-linked network in this patent application are interspersed with each other, and the two are relatively independent, The force between each other is very small, which leads to the problems of low cross-linking density and low mechanical strength in the functionalized double network hydrogel in this patent application; in addition, the functionalized double network hydrogel in this patent application is also There are problems such as excessive swelling rate and cartilage repair effect that need to be further improved

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