Cartilage tissue engineering compound and application thereof

A cartilage tissue and composite technology, applied in the field of biomedical tissue engineering, can solve the problems of limited proliferation of articular cartilage cells, long waiting time for patients, and low strength of cell-material composites.

Pending Publication Date: 2022-08-05
SHANGHAI RESTHETIC BIO CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the chondrocyte-material composite (ACI or MACI) constructed by the existing tissue engineering repair technology has been gradually developed in clinical treatment, there are still many disadvantages that cannot be avoided: 1. The source of the cells is articular cartilage, which is already damaged Obtaining cells from the joint parts of the body will inevitably increase the damage of the original joints
2. The proliferation ability of articular chondrocytes is limited, and the construction period of the cell-material complex in the early stage is long, and patients need to wait for a long time
3. The strength of the cell-material composite is low, far below the level of mechanical strength of natural cartilage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cartilage tissue engineering compound and application thereof
  • Cartilage tissue engineering compound and application thereof
  • Cartilage tissue engineering compound and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0142] Preparation of cartilage gel-demineralized bone complex

[0143] In this example, a cartilage gel-framework complex was prepared. The specific operation method is as follows:

[0144] (1) Take the subject's autologous ear cartilage tissue, aseptically cut 2.5 × 2.5 cm 2 cartilage tissue, use sterile instruments to peel off the mucosa and fibrous tissue on the cartilage surface;

[0145] (2) Cut the cartilage tissue into 1.5×1.5mm 2 Large and small cartilage fragments; the preparation concentration is 0.15% collagenase; the cartilage fragments are added to the prepared collagenase to digest for 8 hours;

[0146] (3) After 8 hours, the collagenase solution was filtered and centrifuged to obtain ear chondrocytes, and primary and subculture were carried out using high glucose DMEM medium containing 10% FBS;

[0147] (4) After the expansion, collect the cells and resuspend them according to 8×10 6 / 10ml to 30×10 6 Cells / 10ml / well were seeded in six-well plates and cult...

Embodiment 2

[0153] Preparation of Perichondrial Sheet Particles-Demineralized Bone Complex

[0154] In this example, perichondrial sheet particle-framework complexes were prepared. The specific operation method is as follows:

[0155] (1) Aseptically cut 2.5×2.5cm 2 ear cartilage tissue; use sterile instruments to peel off the mucosa and fibrous tissue on the cartilage surface;

[0156] (2) Cut the cartilage tissue into 1.5×1.5mm 2 Large and small cartilage fragments; the preparation concentration is 0.15% collagenase; the cartilage fragments are added to the prepared collagenase to digest for 8 hours;

[0157] (3) After 8 hours, the collagenase solution was filtered and centrifuged to obtain ear chondrocytes, and primary and subculture were carried out;

[0158] (4) After the expansion, collect the cells and resuspend them according to 8×10 6 / 10ml to 30×10 6 Cells / 10ml / well were seeded in six-well plates and cultured in gelatinization medium (DMEM medium containing 4-5wt% glucose,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to view more

Abstract

The invention provides a cartilage tissue engineering compound. Specifically, the graft comprises cartilage gel or cartilage membrane particles containing cartilage cells and a porous framework structure, and the cartilage gel or the cartilage membrane particles are loaded on the porous framework structure to form a cartilage gel / cartilage membrane particle-framework structure compound. The invention also provides a preparation method of the cartilage tissue engineering graft and application of the cartilage tissue engineering graft in repair and reconstruction of articular cartilage.

Description

technical field [0001] The invention relates to the field of biomedical tissue engineering, in particular, to a cartilage tissue engineering compound and a preparation method thereof, and its application in joint repair. Background technique [0002] In recent years, with the rapid development of economy and society, various types of articular cartilage lesions such as sports injuries, accidental injuries and joint degenerative diseases have become more and more common. There is a huge clinical need for restoration. At present, the clinical treatment methods for articular cartilage lesions are mainly palliative treatment and repairing treatment. Palliative treatment mainly includes arthroscopic debridement and chondroplasty. These treatments can clean up the uneven cartilage surface of the joint surface and remove cartilage fragments to restore the smoothness of the joint surface. Such methods are less traumatic. It can relieve the symptoms of patients to a certain extent,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61L27/38A61L27/18A61L27/36A61L27/56A61L27/52A61L27/58
CPCA61L27/18A61L27/3608A61L27/3654A61L27/3817A61L27/3852A61L27/56A61L27/52A61L27/58A61L2430/06A61L27/38A61L27/36A61F2/02A61L27/54A61F2/08A61L27/3612A61L27/20A61L27/3834A61L27/3895C12N5/0655C12N2533/90C12N5/0018C12N2500/32C12N2500/38C12N2500/90C12N2501/15C12N2501/39
Inventor 周广东刘豫慈政
Owner SHANGHAI RESTHETIC BIO CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap