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Preparation method of nano micrometer structure coexistence chitosan double-layer support

A micron structure, chitosan technology, applied in medical science, prosthesis, etc., can solve problems that cannot adapt to complex organs and tissue repair of the human body

Active Publication Date: 2013-06-05
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the chitosan biomaterials prepared at present have a single microstructure, which cannot adapt to the repair of complex organs and tissues of the human body. The environment in which bone cells grow

Method used

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  • Preparation method of nano micrometer structure coexistence chitosan double-layer support
  • Preparation method of nano micrometer structure coexistence chitosan double-layer support
  • Preparation method of nano micrometer structure coexistence chitosan double-layer support

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0017] Embodiment: add 2.0g pure acetic acid in the distilled water of 98ml, mix well. Add 6g of chitosan powder to this acetic acid solution, and dissolve it with electromagnetic stirring, and form a uniform viscous solution after 12 hours. Pour the chitosan solution into a 24-well cell culture plate, vacuum out the air bubbles in the solution, and place it in a freezer at -18°C overnight to form a chitosan gel. The gel was freeze-dried for 3 days in a vacuum dryer, taken out, and cut into micron chitosan sheets with a thickness of about 2 mm. Paste micron chitosan flakes on aluminum foil with conductive double-sided adhesive tape. Weigh 0.7 g of chitosan powder and add 9.3 g of trifluoroacetic acid. Dissolve chitosan at 70°C under reflux conditions. Take 2 ml chitosan trifluoroacetic acid solution, add it to a 3 ml needle tube, pass through 15 kV high-voltage static electricity, the distance between the needle tube and the substrate micron chitosan sheet is 20 cm, and the...

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Abstract

The invention relates to a preparation method of a nano micrometer structure coexistence chitosan double-layer support, which belongs to the biology medical material field. The preparation method is characterized in that a phase separating technology is used to prepare a three-dimensional micrometer chitosan support as a basal layer, a high voltage static static spinning technology is used for spinning nano chitosan fiber on the micrometer chitosan surface, and thereby the nano micrometer structure coexistence chitosan double-layer support can be obtained. The prepared double layer support has good mechanical strength and high amount of porosity, and is suitable for adhesion, growth and differentiation of cells. The surface layer chitosan nano fiber is suitable for growth of cartilage cells, the basal layer micrometer chitosan support is suitable for the growth of the osteoblast, and the nano micrometer structure coexistence chitosan double-layer support can be used for restoring joint position of the human body cartilage and hard bone.

Description

technical field [0001] The technical field of the invention relates to biomedical materials, and relates to a preparation method of a chitosan bilayer bracket with coexistence of nano and micro structures. Background technique [0002] Tissue engineering is an interdisciplinary science that applies the principles of cell biology and engineering to research and develop biologically active substitutes for repairing or improving the structure and function of human diseased tissues or organs. Its basic principle and method are to induce differentiation and proliferation of autologous or allogeneic living cells, plant them on a biomaterial with excellent cytocompatibility and can be degraded and absorbed by the body to form a complex, and then combine the cell-biomaterial When biomaterials are implanted into the damaged parts of human tissues and organs, while the biomaterials are gradually degraded and absorbed by the body, the cells continue to proliferate and differentiate, fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/20
Inventor 楼涛汪学军
Owner QINGDAO UNIV
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