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Application of chiral nano bionic photosensitive protein in preparation of medicine for promoting regeneration of damaged neuron axons

A nano-biomimetic, light-sensitive protein technology, applied in the preparation of drugs for promoting the regeneration of damaged neuron axons, in the field of chiral nano-biomimetic light-sensitive proteins, can solve problems such as lack of internal mechanisms, and achieve the goal of relieving central nervous system damage and diseases. Effect

Active Publication Date: 2022-08-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have reported that chiral gold nanofilms can be used for cell growth and differentiation under the action of circularly polarized light, but this study failed to achieve the repair of damaged neurons, as well as the regulation of light-chiral nanomaterials-cells lack of deep explanation for the inner mechanism between

Method used

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  • Application of chiral nano bionic photosensitive protein in preparation of medicine for promoting regeneration of damaged neuron axons
  • Application of chiral nano bionic photosensitive protein in preparation of medicine for promoting regeneration of damaged neuron axons
  • Application of chiral nano bionic photosensitive protein in preparation of medicine for promoting regeneration of damaged neuron axons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Performance regulation of chiral metal hydroxide nanoparticles with near-infrared photoresponse:

[0036] Ligand species optimization during the synthesis of chiral metal hydroxide nanoparticles: the chiral ligands (glutathione, aspartic acid, histidine) were combined with Ni, Cu, and Co ions at a molar concentration ratio of 100 : 1, adjust the pH of the reaction solution to about 8.5, and mix and stir for 12 hours at room temperature to obtain chiral metal hydroxide nanoparticles (GSH-Ni, His-Ni, Asp- Ni, GSH-Cu, Asp-Co), transmission electron microscope (TEM) images of different chiral metal hydroxide nanoparticles are shown in 1. The obtained chiral metal hydroxide nanoparticles synthesized with different ligands and different elements were analyzed by circular dichroism spectroscopy, and the results were as follows figure 2 shown. Depend on figure 2 The results show that the chiral nickel hydroxide nanoparticles (GSH-Ni) synthesized by glutathione ha...

Embodiment 2

[0038] Example 2 Chiral nickel hydroxide nanoparticles repair damaged neuron axon regeneration

[0039] 40 μg of chiral nickel hydroxide nanoparticles were injected into AD mice through the tail vein, once every 5 days for two consecutive months, and a 980 nm laser (600 mW / cm 2 ) for two consecutive months (12h of light per day). Detection of Ni in AD mouse brain at different time points (6h, 12h, 24h, 2d, 3d, 7d, 15d, 30d, 45d, and 60d) by inductively coupled plasma mass spectrometry (ICP-MS) The content of elements, the enrichment statistics of multiple injections of chiral nickel hydroxide nanoparticles in the brain of AD mice are as follows Figure 4 shown. Depend on Figure 4 It was found that with the increase of injection times, the content of Ni in the brain gradually increased, indicating that nickel hydroxide nanoparticles can efficiently cross the blood-brain barrier.

[0040] The neurons in the hippocampus of wild-type mice (WT), AD mice, and L-type, DL-type, D-t...

Embodiment 3

[0041] Example 3 Chiral nickel hydroxide nanoparticles reduce the content of Aβ and p-Tau protein in the cerebrospinal fluid of Alzheimer's mice under the action of near-infrared light, and relieve motor and cognitive impairment

[0042] 40 μg of chiral nickel hydroxide nanoparticles were injected into AD mice through the tail vein, once every 5 days for two consecutive months, and a 980 nm laser (600 mW / cm 2 ) for two consecutive months (12h of light per day). Whether the motor and cognitive abilities of wild-type mice (WT), untreated AD mice, L-type, DL-type, D-type nickel hydroxide nanoparticles and AD mice treated with near-infrared light were improved by water maze test Improvement, the behavioral statistics of AD mice in different groups are as follows Image 6 shown. Depend on Image 6 The results showed that after training, AD mice injected with D-type nickel hydroxide nanoparticles and treated with near-infrared light had the shortest time to find the target platfo...

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Abstract

The invention relates to application of chiral nano bionic photosensitive protein in preparation of a medicine for promoting regeneration of axons of damaged neurons. Chiral nickel hydroxide ultra-small nanoparticles with characteristics similar to photosensitive protein are utilized, can penetrate through a blood brain barrier, enter damaged neuronal cells in the brain of an AD mouse and are combined with a cell membrane receptor, calcium ion flow is caused under the action of near-infrared light, and then a Wnt5a / JNK pathway is activated. Through illumination treatment for two months, the expression quantities of RhoA and ROCK in the brain of the AD mouse are reduced, axons of damaged neurons regrow, meanwhile, the content of marker protein in cerebrospinal fluid of the AD mouse is reduced, and the memory and cognitive abilities are recovered. The method can be applied to neuronal repair of AD model mice, and provides possibility for relieving central nervous system diseases such as neuronal injury.

Description

technical field [0001] The invention relates to the technical field of material chemistry, in particular to the application of a chiral nanometer biomimetic light-sensitive protein in the preparation of a drug for promoting regeneration of damaged neuron axons. Background technique [0002] Neurons are the basic units of the structure and function of the nervous system, regulating and controlling the life activities and health of the human body. Once damaged, it will cause permanent damage to the brain, resulting in motor, memory and cognitive dysfunction, and repair and regeneration of damaged neurons. Essential for maintaining normal neural activity. Therefore, establishing a feasible method that can enhance the intrinsic regenerative ability of neurons and enable the regeneration and functional recovery of damaged neuron axons has great application prospects in alleviating central nervous system injury diseases such as Alzheimer's disease. In recent years, with the devel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K33/24A61P25/28B82Y5/00B82Y40/00
CPCA61K41/0042A61K33/24A61P25/28B82Y5/00B82Y40/00A61K2300/00
Inventor 胥传来瞿爱华匡华孙茂忠徐丽广郝昌龙刘丽强宋珊珊胡拥明
Owner JIANGNAN UNIV
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