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Bacillus and application thereof in preventing and treating plant diseases

A technology for bacillus and plant diseases, applied in the field of microorganisms, can solve problems that threaten food safety, environmental pollution, water pollution, etc., and achieve the effect of broad-spectrum antibacterial performance

Pending Publication Date: 2022-07-29
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chemical control is the oldest control method used by humans, but the extensive use of chemical pesticides and fertilizers has led to environmental pollution, soil compaction, water pollution, a large number of beneficial microorganisms are killed by pesticides, and environmental problems such as pathogenic bacteria are easy to develop drug resistance. Highlight, And, Chemical Pesticide Residues Threaten Food Safety

Method used

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  • Bacillus and application thereof in preventing and treating plant diseases
  • Bacillus and application thereof in preventing and treating plant diseases
  • Bacillus and application thereof in preventing and treating plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Example 1. Isolation, identification and preservation of Bacillus sp. YJD-SF1

[0092] 1.1. Isolation of bacterial YJD-SF1

[0093] 1.1.1. Add 5g of soil samples to 45mL of sterile distilled water (collected from No. 6 Anfa Avenue, Economic and Technological Development Zone, Anda City, Suihua City, Heilongjiang Province, saline-alkali soil plant rhizosphere soil), stir for 15min, stand for 10min, and then take the soil sample. Add 1 mL of supernatant to a sterile test tube containing 9 mL of sterile water and mix thoroughly (the dilution at this time is recorded as 10 -1 ), then draw 1 mL from this test tube and add it to another sterile test tube containing 9 mL of sterile water to mix evenly, and so on to make 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 Bacterial suspensions at different dilutions. 0.1 mL of each dilution was evenly spread on LB solid medium, and cultured at a constant temperature of 30°C for 2-3 days.

[0094] 1.1.2. After completing step 1.1.1, pi...

Embodiment 2

[0121] Embodiment 2, the preparation of YJD-SF1 bacterial agent

[0122] Pick a single colony that was cultured in TSB solid medium for three days and inoculated into 100 mL of TSB liquid medium, and cultured at 28°C and 140r / min for 48h to obtain a bacterial solution with an OD600 value of about 1.0 (2×10 8. CFU / mL).

[0123] The bacterial liquid is the prepared YJD-SF1 bacterial agent.

[0124] The TSB formula and configuration method are: casein trypsin digest 17.0g, soybean flour papain digest 3.0g, dipotassium hydrogen phosphate 2.5g, sodium chloride 5.0g, glucose 2.5g, distilled water to 1000mL, pH Adjust to 7.3 and sterilize at 121°C for 15min.

Embodiment 3

[0125] Example 3. Determination of Bacillus YJD-SF1 antibacterial broad-spectrum

[0126] 3.1. Test pathogens:

[0127] Rice sheath blight - Rhizoctonia solani, Phytophthorasojae, Alternaria tenuissima

[0128] 3.2. The antibacterial experiments were carried out on the above-mentioned plant pathogens by the plate confrontation culture method.

[0129] The specific operation was as follows: Bacillus YJD-SF1 was incubated on LB solid medium at 28°C for 48h at constant temperature. The above-mentioned test plant pathogenic bacteria were cultured on a PDA plate at a constant temperature of 25°C-28°C for 3-4 days, and a sterile stainless steel hole punch with a diameter of 0.6 cm was used from the edge of the colony to make pathogenic bacteria agar sheets; use sterile tweezers to pick pathogenic bacteria. Slices, the mycelium was inoculated at the center of the PDA flat plate, and at the same time about 2.50cm from both sides of the pathogenic bacteria sheet, with aseptic inocula...

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Abstract

The invention discloses bacillus and application thereof in preventing and treating plant diseases, and belongs to the technical field of microorganisms. The invention discloses bacillus, which is bacillus sp., the strain number of the bacillus sp. Is YJD-SF1, and the preservation number of the bacillus sp. In the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center). 24120. The invention further discloses application of the bacillus in prevention and treatment of alternaria tenuissima, rhizoctonia solani and phytophthora sojae. The bacillus YJD-SF1 disclosed by the invention has a stable and broad-spectrum antibacterial property, and has an inhibiting effect on three plant pathogenic fungi, namely alternaria tenuissima, phytophthora sojae and rhizoctonia solani.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Bacillus strain and its application in preventing and treating plant diseases. Background technique [0002] Chemical control is the oldest control method used by humans, but the extensive use of chemical pesticides and fertilizers has led to environmental pollution, soil compaction, and water pollution. Prominently, moreover, chemical pesticide residues threaten food safety. As an environmentally friendly alternative method emerging in recent years, biological control uses growth-promoting bacteria that can promote crop growth and development or YJD-SF1 inoculant that inhibits pathogen populations to effectively improve crop yields or control plant diseases. Some plant rhizosphere microorganisms may have the characteristics of YJD-SF1 inoculant and growth-promoting bacteria at the same time, which can be used as a more effective tool to control phytopathoge...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/26C12N9/54C12N9/78C12P17/10A01N63/22A01P3/00A01P21/00C12R1/07
CPCC12N1/20C12N9/2411C12N9/54C12N9/78C12P17/10C12Y305/99007A01N63/22
Inventor 李立新徐嘉莉王茂淋薛逢伯冯献忠王东梅张荣淑李忠悦宋扬
Owner NORTHEAST FORESTRY UNIVERSITY
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