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Cultivation method of transgenic oyster mushroom Pofst3 +

A cultivation method and transgenic technology, applied in mushroom cultivation, cultivation, plant cultivation, etc., can solve the problems of poor uniformity of mushroom production, low biological efficiency, late start of factory cultivation of oyster mushrooms, etc., and achieve good uniformity of mushroom production , ensure safety, and improve biological efficiency

Pending Publication Date: 2022-07-26
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In summary, the problems in the prior art are: flat mushroom industrial cultivation starts late, the flat mushroom varieties that are suitable for industrial cultivation are to be selected and selected, and relevant compost formula, The preparation process, fungus bag making, mycelia culture, bud-inducing process, and fruiting management are not suitable for the industrial cultivation of Pleurotus ostreatus, and the biological efficiency is low, and the uniformity of fruiting is poor.

Method used

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  • Cultivation method of transgenic oyster mushroom Pofst3 +

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this example, transgenic oyster mushroom Pofst3 + The cultivation method includes the following steps:

[0024] S1, prepare a strain medium to obtain a cultivar; Described preparation strain medium: the mother seed medium is peptone 2.0g, yeast extract 2.0g, MgSO 4 ·7H 2 O 0.5g, KH 2 PO 4 0.46g, K 2 HPO 4 1 g, 20 g of glucose and 20 g of agar powder, add 1000 mL of deionized water, and sterilize at a temperature of 115°C for 30 minutes; both the original seed and the cultivar use the wheat grain medium: 98 kg of high-quality wheat, 1 kg of sucrose, and 1 kg of calcium carbonate ;

[0025] The production process of the mother seed: The prepared mother seed medium is divided into test tube slants. After the mother seed medium is solidified, the transgenic oyster mushroom Pofst3 stored in the low-temperature refrigerator is taken out. + The strains were inoculated into test tubes and placed in an incubator for two weeks in the dark at a temperature of 22°C to 24°...

Embodiment 2

[0033] In this example, transgenic oyster mushroom Pofst3 + The cultivation method includes the following steps:

[0034] S1, prepare a strain medium to obtain a cultivar; Described preparation strain medium: the mother seed medium is peptone 2.0g, yeast extract 2.0g, MgSO 4 ·7H 2 O 0.5g, KH 2 PO 4 0.46g, K 2 HPO 4 1 g, 20 g of glucose and 20 g of agar powder, add 1000 mL of deionized water, and sterilize at a temperature of 115°C for 30 minutes; both the original seed and the cultivar use the wheat grain medium: 98 kg of high-quality wheat, 1 kg of sucrose, and 1 kg of calcium carbonate ;

[0035] The production process of the mother seed: The prepared mother seed medium is divided into test tube slants. After the mother seed medium is solidified, the transgenic oyster mushroom Pofst3 stored in the low-temperature refrigerator is taken out. + Bacteria, inoculated in test tubes, placed in an incubator, and cultured for two weeks in the dark at a temperature of 22°C;

...

Embodiment 3

[0043] In this example, transgenic oyster mushroom Pofst3 + The cultivation method includes the following steps:

[0044] S1, prepare a strain medium to obtain a cultivar; Described preparation strain medium: the mother seed medium is peptone 2.0g, yeast extract 2.0g, MgSO 4 ·7H 2 O 0.5g, KH 2 PO 4 0.46g, K 2 HPO 4 1 g, 20 g of glucose and 20 g of agar powder, add 1000 mL of deionized water, and sterilize at a temperature of 115°C for 30 minutes; both the original seed and the cultivar use the wheat grain medium: 98 kg of high-quality wheat, 1 kg of sucrose, and 1 kg of calcium carbonate ;

[0045] The production process of the mother seed: The prepared mother seed medium is divided into test tube slants. After the mother seed medium is solidified, the transgenic oyster mushroom Pofst3 stored in the low-temperature refrigerator is taken out. + Bacteria, inoculated in test tubes, placed in an incubator, and cultured for two weeks in the dark at a temperature of 24°C;

...

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Abstract

The invention provides a method for cultivating transgenic oyster mushroom Pofst3 +, which comprises the following steps of: preparing a strain culture medium to obtain cultivated species; preparing a culture material, putting the culture material into a culture bottle, and sterilizing the culture bottle; then filling the cultivated species into a sterilized culture bottle to obtain a fungus bottle; the fungus bottle is placed in a culture room for mycelium culture, and then fruiting period management is conducted. According to the cultivation method of the transgenic oyster mushroom Popst3 +, the number of primordia of the transgenic oyster mushroom Popst3 + can be appropriate, the fruiting uniformity is high, and the requirement for the oyster mushroom variety in industrialized production of the oyster mushroom can be met.

Description

technical field [0001] The invention belongs to the technical field of bacterial culture, and in particular relates to a transgenic oyster mushroom Pofst3 + cultivation method. Background technique [0002] Oyster mushroom is one of the most widely cultivated edible fungi in my country. At present, there are few factory production methods of oyster mushroom in my country. The production cycle of oyster mushrooms is short, suitable for factory cultivation, which can ensure the daily supply of oyster mushrooms in the market, and the product quality and safety are also relatively guaranteed, which is the main direction for the development of oyster mushroom cultivation in the future. In factory cultivation, most of the key technical parameters are kept secret, which to a certain extent affects the progress of oyster mushroom factory production technology. In particular, parameters such as production mode, mycelium culture and bud-inducing environmental conditions, and fruiti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/40A01G18/20
CPCA01G18/00A01G18/40A01G18/20
Inventor 戚元成申进文王贺莲侯晓娟文晴胡延如
Owner HENAN AGRICULTURAL UNIVERSITY
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