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Biomarker for diagnosis of preeclampsia of pregnancy, application and kit

A diagnostic kit and biomarker technology, applied in the field of biomedicine, can solve problems such as non-compliance with diagnostic standards, and achieve the effects of strong specificity and high detection sensitivity

Pending Publication Date: 2022-07-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, HELLP syndrome (hemolysis, elevated liver transaminases, and thrombocytopenia) in some patients is also considered a form of preeclampsia, but it does not meet typical diagnostic criteria

Method used

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  • Biomarker for diagnosis of preeclampsia of pregnancy, application and kit
  • Biomarker for diagnosis of preeclampsia of pregnancy, application and kit
  • Biomarker for diagnosis of preeclampsia of pregnancy, application and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This program detects the concentrations of αPS antibody and its subtypes IgA, IgG, and IgM in the plasma of pregnant women with preeclampsia (12 weeks of gestation) and healthy pregnant women (12 weeks of gestation), that is, the concentrations of αPSIgA, αPSIgG, and αPSIgM. Correlation analysis between autoantibody levels and clinical indicators, including the following experimental steps:

[0027] 1. Collection of serum samples: blood samples were collected with vacuum coagulant blood collection tubes, and fresh whole blood ( 12 weeks of pregnancy), mix up and down, let stand at room temperature for 30 minutes, centrifuge at 3000 rpm for 5 minutes, draw the upper serum for detection, obtain plasma samples, select one of the plasma samples of pregnant women with preeclampsia and PBS at 1:10, 1:50, The ratios of 1:100, 1:200, 1:400 and 1:800 were diluted and stored for later use. The remaining samples were diluted with PBS at the ratio of 1:100 and stored for later use....

Embodiment 2

[0037] This protocol detects the concentration of total IgG in the plasma of pregnant women with preeclampsia (12 weeks of gestation) and healthy pregnant women in the first trimester (12 weeks of gestation), including the following steps:

[0038] 1. Collection of serum samples: blood samples were collected with vacuum coagulant blood collection tubes, and fresh whole blood ( 12 weeks of pregnancy), mixed up and down, left at room temperature for 30 minutes, centrifuged at 3000 rpm for 5 minutes, sucked the upper serum for detection, and obtained plasma samples. The samples were diluted 1:100 with PBS and stored for future use.

[0039] 2. Antigen coating: 100ug of Purified anti-human IgG Fc Recombinant Antibody (BioLegend, California, USA, 366902) was added to PBS and diluted to a final concentration of 5ng / ml; 60ul of PS antigen was added to each well of the ELISA plate; sealed 96 The well plate was placed in a 4°C refrigerator for overnight incubation; the 96-well plate w...

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PUM

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Abstract

The invention discloses a biomarker for diagnosis of preeclampsia of pregnancy, application and a kit, and relates to the field of biological medicines. According to the application, the relevance between the alpha PS IgG antibody level and the preeclampsia blood coagulation function is found, and compared with a healthy control, the plasma alpha PS IgG antibody of a preeclampsia patient is increased by 0.47 times, so that the difference is obvious. The alpha PS IgG antibody can be used as a biomarker for diagnosis of preeclampsia in pregnancy, detection of the alpha PS IgG antibody can be possibly applied to clinical diagnosis of preeclampsia in the future, and the alpha PS IgG antibody can also be used as an effective supplementary means for screening and diagnosis of preeclampsia at present. And compared with the existing total IgG detection, the alphaPS antibody has higher specificity and higher detection sensitivity.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a biomarker for diagnosing preeclampsia in pregnancy and its application and kit. Background technique [0002] Preeclampsia (preeclampsia) is a systemic vascular disease characterized by new-onset hypertension and proteinuria after 20 weeks of gestation. It originates from the maternal-fetal interface and affects multiple organ systems. Preeclampsia progresses rapidly, and there is currently no effective treatment other than timely termination of pregnancy. Therefore, preeclampsia causes an increase in the proportion of iatrogenic premature birth and cesarean section, which seriously damages the health of mothers and babies. At present, clinical biomarkers that can be used to predict and diagnose preeclampsia in the first trimester are still very scarce, so it is impossible to effectively screen high-risk groups of preeclampsia. The pathogenesis of preeclampsia includes abnormal plac...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/689
Inventor 马孔阳秦任材郑智华关慧
Owner SUN YAT SEN UNIV
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