Method for screening and culturing denitrifying bacteria flora with high salt tolerance

A denitrifying bacteria, screening and culturing technology, applied in chemical instruments and methods, methods based on microorganisms, methods using microorganisms, etc., can solve the problems of complex composition, high salt content, high pollution intensity, etc. Steady growth, high-efficiency denitrification, and biomass enhancement

Pending Publication Date: 2022-07-15
BEIJING UNIV OF TECH
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Problems solved by technology

[0003] With the rapid development of my country's industrialization process, the discharge of industrial comprehensive wastewater is also increasing. Affected by its own water quality, industrial comprehensive wastewater has the characteristics of complex pollutant components, high pollution intensity, and high salt content. The denitrification treatment of the
At present, the activated sludge denitrification process is still adopted by most sewage treatment plants. The activated sludge is a single sludge system with complex composition. Bacteroid growth requirements, sludge nitrogen removal capacity is severely limited
In addition, the high salinity environment of industrial wastewater is not conducive to the growth of conventional denitrification bacteria, and has a more obvious adverse effect on biological denitrification

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  • Method for screening and culturing denitrifying bacteria flora with high salt tolerance

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Embodiment 1

[0020] In this case, the screening and cultivation of industrial grade is carried out. For the device, see figure 1 , the effect is remarkable, the specific content is as follows:

[0021] (1) Material preparation: The experimental equipment is an industrial-grade biological fermenter with a volume of 3000L, and the effective volume is controlled to 2500L during the actual operation. The return sludge from the secondary sedimentation tank of the sewage treatment plant was used as the seed sludge, and the sludge concentration was MLSS=3339mg / L. The prepared concentrations of nitrate nitrogen, COD and phosphorus were 13000mg / L, 39000-45500mg / L and 130mg / L respectively. In addition, the added trace element solution composition is specifically: ZnSO 4 ·7H 2 O is 0.12mg / L, Na 2 MoO 4 ·2H 2 O is 0.12mg / L, CoCl 2 ·6H 2 O is 0.15mg / L, FeCl 3 ·6H 2 O is 1.50mg / L, CuSO 4 ·5H 2 O is 0.03mg / L, NiCl 2 ·6H 2 O is 0.12mg / L, Mn 2 SO 4 ·H 2O is 0.12mg / L, and 1mL of trace eleme...

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Abstract

The invention discloses a high-salt-tolerance denitrifying bacteria flora screening culture method, and belongs to the field of sewage and wastewater treatment. Return sludge of a secondary sedimentation tank of a sewage treatment plant is used as inoculation sludge, substances such as a carbon source, a nitrogen source and trace elements required by microorganisms are artificially prepared, a culture environment with the salinity value of 4% is established and maintained by utilizing high-concentration substrate feeding and intermittent operation, and operation parameters such as pH and temperature are strictly controlled; and the water inlet load is adjusted by detecting various indexes of the effluent, so that the denitrifying bacteria flora with high salt tolerance is successfully screened and cultured.

Description

technical field [0001] The invention belongs to the field of sewage and waste water treatment, and particularly relates to screening and culturing a denitrifying bacterial flora with high salt tolerance. The bacteria group (the first dominant functional bacteria genus is Halomonas), the culture of the bacteria group has broad application prospects for the production of embedded bioactive fillers and the improvement of the denitrification performance of high-salinity industrial wastewater. Background technique [0002] Heterotrophic denitrification plays a crucial role in the denitrification of various types of sewage and wastewater. This reaction is able to oxidize organic matter under anoxic conditions and use nitrate nitrogen as the The electron acceptor is reduced to gaseous nitrogen which is harmless to the human body and the environment. [0003] With the rapid development of my country's industrialization process, the discharge of industrial comprehensive wastewater i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20C02F3/34C12R1/01C02F101/16
CPCC12N1/36C12N1/20C02F3/34C02F2101/163C02F2101/166
Inventor 杨宏张鑫
Owner BEIJING UNIV OF TECH
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