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Fatty liver and obesity relieving metaplasm prepared from lactobacillus rhamnosus and application of metaplasm

A Lactobacillus rhamnosus, epigenetic technology, applied in the fields of medicine and microbiology, can solve the problems of increasing liver burden and liver toxicity, and achieve the effects of improving blood sugar homeostasis, promoting browning, and reducing cell size

Active Publication Date: 2022-07-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, drugs are mainly detoxified and metabolized in the liver. Excessive or inappropriate drug intake will increase the burden on the liver and liver toxicity. Therefore, drugs should be carefully selected for the treatment of fatty liver

Method used

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  • Fatty liver and obesity relieving metaplasm prepared from lactobacillus rhamnosus and application of metaplasm
  • Fatty liver and obesity relieving metaplasm prepared from lactobacillus rhamnosus and application of metaplasm
  • Fatty liver and obesity relieving metaplasm prepared from lactobacillus rhamnosus and application of metaplasm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Screening and identification of Lactobacillus rhamnosus

[0076] Specific steps are as follows:

[0077] 1. Filter

[0078] The sample was obtained from the feces of healthy people in Shanghai. After the sample was pretreated, it was stored in a -80°C refrigerator in 20% glycerol. After being taken out and thawed, it was mixed and added to 4.5mL of normal saline to contain 9g / L normal saline for gradient dilution, select a suitable gradient dilution and spread it on MRS solid medium, culture at 37°C for 48 hours, pick a typical colony of Lactobacillus rhamnosus onto MRS solid medium for streak purification, pick A single colony was transferred to MRS liquid medium for enrichment and preserved in 30% glycerol to obtain a strain named CCFM1219; among them, the typical colony of Lactobacillus rhamnosus was round, white and smooth.

[0079] 2. Identification

[0080] The genome of strain CCFM1219 was extracted, the 16S rDNA of strain CCFM1219 was amplified an...

Embodiment 2

[0081] Example 2: Post-production of Lactobacillus rhamnosus CCFM1219

[0082] The MRS solid medium was cultured in a water-proof constant temperature incubator at 37°C for 24-48h to obtain a single colony; the single colony was picked and inoculated into the MRS liquid medium, and cultured at 37°C for 12h to obtain culture solution 1; 1 was inoculated into MRS liquid medium with 1% (v / v) inoculum, cultured at 37°C for 12 h to obtain culture medium 2; inoculated with 1% (v / v) of culture medium 2 to the prepared epiphyte. Seed liquid was obtained from the fermentation medium (HSY medium) of Yuanyuan, and cultivated at 37 °C for 12 h; the seed liquid was inoculated into HSY medium at 3-5% (v / v), and cultivated at 37 °C for 24 hours. -36h to get bacterial liquid.

[0083] After heat treatment (65° C., 30 min) of the bacterial liquid, the postbiotic fermentation broth was obtained, and the postbiotic lyophilized powder was obtained by freeze-drying.

[0084] The HSY medium was f...

Embodiment 3

[0085] Example 3: Effects of postbiotics prepared by Lactobacillus rhamnosus CCFM1219 on the weight gain of high-fat diet mice

[0086] Sixty 7-week-old healthy male C57BL / 6N mice were randomly divided into 10 groups with 6 mice in each group. The 10 groups were: blank group (Control), model group (Model), HSY medium group: M_1 (low) Dosage: 200mg / kg mouse body weight), M_2 (high dose: 800mg / kg mouse body weight), Lactobacillus rhamnosus CCFM1219 Postbiotic group: CCFM1219_1 (low dose: 200mg / kg mouse body weight), CCFM1219_2 (high dose : 800 mg / kg mouse body weight), Lactobacillus rhamnosus FTJDG postbiotic group: FTJDG_1 (low dose: 200 mg / kg mouse body weight), FTJDG_2 (high dose: 800 mg / kg mouse body weight), Lactobacillus paracasei CQYY Epigenetic group: CQYY_1 (low dose: 200 mg / kg mouse body weight), CQYY_2 (high dose: 800 mg / kg mouse body weight).

[0087] The experiment lasted for 13 weeks: after the mice were adapted for one week, the blank group was fed with low-fat a...

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Abstract

The invention discloses a postbiotic prepared from lactobacillus rhamnosus and used for relieving fatty liver and obesity and application of the postbiotic, and belongs to the technical field of microorganisms and the technical field of medicines. The metagen prepared from the rhamnobacter rhamnosus CCFM1219 provided by the invention has the effects of relieving fatty liver and obesity: (1) the weight gain of high-fat diet mice is obviously relieved; (2) the blood glucose steady state of high-fat diet mice is obviously improved; (3) the liver fat accumulation and lesion of the high fat diet mouse are obviously inhibited; (4) the size of white fat cells is obviously reduced, the normal form of brown fat is maintained, and the browning of the white fat is promoted; (5) the levels of serum TC, GGT and ALP of high-fat diet mice are obviously reduced; and (6) the weight of white adipose tissues of the liver and the abdomen of the high-fat diet mouse is effectively reduced. Therefore, the metagen prepared from the lactobacillus rhamnosus CCFM1219 has a huge application prospect in preparation of products for preventing and / or treating fatty liver and obesity and even preventing and / or treating obesity and metabolic disorder related diseases.

Description

technical field [0001] The invention relates to a postbiotic for relieving fatty liver and obesity prepared by Lactobacillus rhamnosus and its application, belonging to the technical field of microorganisms and medical technology. Background technique [0002] Obesity is a chronic, complex, and heterogeneous disease that can cause more than 200 medical conditions that affect entire organ systems. Obesity rates have doubled in 73 countries since 1980, with high BMI (>25kg / m 2 ) cause approximately 4 million deaths worldwide and cause a significant burden of cardiovascular disease. With the increasing number of obese patients and the huge hidden dangers to health caused by obesity-related complications, the need for obesity treatment is becoming more and more urgent. The current main ways to lose weight include exercise, diet control, and the use of diet pills. International mainstream weight loss drugs, such as phentermine, orlistat, etc., all have large adverse reactio...

Claims

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Application Information

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IPC IPC(8): C12N1/20
CPCA61K35/747A61K9/14A61K9/19A61K9/08A61P1/16A61P3/04A61P3/10A61P21/00A61P19/08A61P9/00A61P35/00A61P3/06A61P3/00A23L33/135A23V2002/00A23V2400/175A23V2200/30A23V2200/308A23V2200/326A23V2200/3262A23V2200/328A23V2200/332Y02A50/30
Inventor 崔树茂潘正浩张秋香唐鑫毛丙永杨波赵建新陈卫
Owner JIANGNAN UNIV
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