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Lipopolysaccharide/indocyanine green/oxaliplatin nanoparticles and preparation method thereof

A technology of oxaliplatin and indocyanine green, applied in the field of biomedicine, can solve the problems of limited application, achieve the effect of enhancing anti-tumor immune effect and improving tumor immune microenvironment

Pending Publication Date: 2022-07-05
THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the low penetration depth and tumor hypoxic zone limit its application

Method used

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  • Lipopolysaccharide/indocyanine green/oxaliplatin nanoparticles and preparation method thereof
  • Lipopolysaccharide/indocyanine green/oxaliplatin nanoparticles and preparation method thereof
  • Lipopolysaccharide/indocyanine green/oxaliplatin nanoparticles and preparation method thereof

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Embodiment 1

[0030] The specific preparation process of the oxygen-carrying lipopolysaccharide / indocyanine green / oxaliplatin nanoparticles of the present embodiment is as follows:

[0031]S1, weigh 5gDPPC, 2gDPPG, 2gDSPE-mPEG2000 and 1g cholesterol, dissolve in 10ml mixed organic solution of chloroform and methanol (4:1, v / v), to obtain final product;

[0032] S2, the product obtained from S1 is rotary evaporated under the conditions of a water bath at 52° C. and a rotating speed of 100 rpm, until the organic solvent in the flask is completely volatilized and a layer of uniform and transparent lipid film is left on the flask, that is, it is obtained;

[0033] S3, eluted with 10ml of aqueous solution containing 0.1mg / ml lipopolysaccharide, and resuspended for later use;

[0034] S4. Under the conditions of ice bath and light-proof, fill 200 μl perfluoropentane with oxygen to saturation, then drop the oxygen-carrying perfluoropentane into 6 mg / ml oxaliplatin aqueous solution, and then sonica...

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Abstract

The lipopolysaccharide / indocyanine green / oxaliplatin nanoparticle is characterized in that a PLGA inner core is loaded with oxygen-carrying perfluoropentane, indocyanine green and oxaliplatin molecules, and the outer part of the PLGA inner core is tightly coated with a layer of a material consisting of dipalmitoyl phosphatidylcholine, 1, 2-dipalmitoyl phosphatidylcholine and oxaliplatin. The lipid membrane is composed of 1, 2-palmitoyl phosphatidyl glycerol, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and cholesterol, and lipopolysaccharide is loaded on the surface of the lipid membrane. The preparation method is simple. The oxygen-carrying lipopolysaccharide / indocyanine green / oxaliplatin nanoparticles have good particle size potential, dispersibility, encapsulation efficiency, drug loading capacity and optical properties, have no obvious toxic effect on organisms, and have great popularization and application values in improvement of tumor immune microenvironment and anti-tumor immunotherapy.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to an oxygen-carrying lipopolysaccharide / indocyanine green / oxaliplatin nanoparticle and a preparation method and application thereof. Background technique [0002] Cancer immunotherapy is transforming cancer treatment as a revolutionary treatment by exploiting the specificity of the immune system to exert anti-tumor immune effects. However, there is a major obstacle to the immunotherapy of ovarian cancer, that is, the immunosuppressive state of the tumor immune microenvironment. The immune escape caused by the immunosuppressive state of the tumor microenvironment is an important factor for tumor recurrence and metastasis. The tumor immune microenvironment is involved in many functional pathways of immunosuppression, hindering anti-tumor immune responses, including altering stromal cell function to promote tumor growth, altering angiogenesis patterns, the existence of a multi-dif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/555A61K31/739A61K33/00A61K9/51A61K47/34A61P35/00A61K49/22
CPCA61K41/0057A61K31/555A61K31/739A61K33/00A61K9/5192A61K9/5153A61P35/00A61K49/225A61K2300/00
Inventor 李晨阳常淑芳
Owner THE SECOND AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIV
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