Extraction process and application of stauntonia latifolia pericarp soluble dietary fibers with blood glucose reducing function
A technology of dietary fiber and extraction process, which is applied in the field of soluble dietary fiber processing and preparation, can solve problems such as waste of August melon peel and the like
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Embodiment 1
[0040] like figure 1 As shown, a process for extracting soluble dietary fiber from August melon peel with hypoglycemic function includes the following steps:
[0041] Step 1. Crushing and extraction: The peel of August melon is the peel of fresh and mature August melon. After washing and drying the water naturally, it is dried at 50 ° C for 24 hours, crushed and passed through a 60-mesh sieve to obtain August melon. Peel powder; extract by mixing August melon pericarp powder with phosphate buffer, wherein the material-liquid ratio of August melon pericarp powder and phosphate buffer is 1:20 (g / mL).
[0042] Step 2, papain enzymolysis: adjust the pH of the August melon pericarp buffer solution extract to 7, add 600U / g papain, put it into a water bath with a temperature of 70 ° C and heat for 30 minutes, and heat the water bath to 100 °C. The enzyme was inactivated at 100°C for 10 min, and then cooled naturally at room temperature.
[0043] Step 3, α-amylase enzymatic hydrolys...
experiment example 1
[0048] Experimental example 1: Effect of solid-liquid ratio on the extraction of soluble dietary fiber from August melon peel
[0049] 1. Experimental method
[0050] Take 2 g of August melon peel, the ratio of material to liquid is 1:10, 1:15, 1:20, 1:25, 1:30 (g / mL) and other conditions are fixed and unchanged with Example 1, respectively explore In the change of the yield of soluble dietary fiber in August melon peel with different solid-liquid ratios, each group was repeated three times.
[0051] 2. Experimental results
[0052] The result is as figure 2 It is known that the ratio of solid to liquid has a significant effect on the extraction rate of SDF from August melon peel (P<0.05). g / mL), the extraction amount of soluble dietary fiber from August melon peel was significantly higher than that when the solid-liquid ratio was 1:10, 1:15, 1:25, 1:30 (g / mL) . Therefore, in this extraction condition, 1:20 (g / mL) was the best solid-liquid ratio for the extraction of sol...
experiment example 2
[0053] Experimental example 2: The effect of enzymatic hydrolysis temperature of cellulase on the extraction of soluble dietary fiber from August melon peel
[0054] 1. Experimental method
[0055] Take 2 g of August melon peel, the enzymatic hydrolysis temperatures were 40, 45, 50, 55, and 60 °C, and other conditions were the same as those in Example 1. The change of fiber yield was repeated three times for each group.
[0056] 2. Experimental results
[0057] The result is as image 3 It is known that the enzymatic hydrolysis temperature has a significant effect on the extraction rate of SDF from August melon peel (P<0.05). The amount of soluble dietary fiber extracted from moon melon peel was significantly higher than that of soluble dietary fiber when the enzymatic hydrolysis temperature was 40, 45, 55, and 60 ℃. Therefore, in this extraction condition, 50℃ was the optimal enzymatic hydrolysis temperature for the extraction of soluble dietary fiber from August melon pe...
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