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Composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines and application thereof

A technology of stem cells and cytokines, applied in the field of stem cells, can solve the problems of large nitrogen consumption, increase in cost, and restrictions on promotion and application, and achieve the effects of reducing production costs, increasing yield, and improving capacity

Pending Publication Date: 2022-06-03
岳俊丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Umbilical cord mesenchymal stem cells are cultured by conventional methods, and the content of cytokines in the culture medium is low. In order to increase the content of cytokines, it is necessary to treat umbilical cord mesenchymal stem cells with hypoxia during the culture process, but hypoxic treatment of umbilical cord mesenchymal stem cells Requires special equipment such as a hypoxic cell incubator
Moreover, a large amount of nitrogen gas needs to be consumed in the hypoxic treatment process, resulting in a further increase in cost, which limits its popularization and application to a certain extent.

Method used

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  • Composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines and application thereof

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Experimental program
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Effect test

Embodiment 1

[0024] A composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines, comprising the following components by weight: 160 parts of DMEM / F12 medium, 4.5 parts of L-glutamine, 2.6 parts of iron amaranth extract, and 8 parts of vitamin C , 4.4 parts of huperzine A, and 12 parts of insulin; the extract of Amaranthus amaranthus is an extract in a ratio of 20:1, and the average particle size of the extract is 80-120 mesh.

[0025] The application of the above-mentioned composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines includes the following steps:

[0026] (1) Add L-glutamine, amaranth extract, vitamin C, huperzine A, and insulin to DMEM / F12 medium, mix well to obtain induction medium, store at 4°C for later use

[0027] (2) The primary isolated umbilical cord mesenchymal stem cells were seeded in 8 mL of complete medium (DMEM / F12 medium, 5% FBS, 0.5 mg / mL human serum albumin, 5 μg / mL insulin, 50 U / ml penicillin) , the seedin...

Embodiment 2

[0030] A composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines, comprising the following components by weight: 150 parts of DMEM / F12 medium, 3.5 parts of L-glutamine, 1.4 parts of iron amaranth extract, 7.5 parts of vitamin C parts, 3.2 parts of huperzine A, and 10 parts of insulin; the Amaranthus amaranthus extract is the extract in the ratio of 20:1, and the average particle size of the extract is 80-120 mesh.

[0031] The application of the above-mentioned composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines includes the following steps:

[0032] (1) Add L-glutamine, amaranth extract, vitamin C, huperzine A, and insulin to DMEM / F12 medium, mix well to obtain induction medium, store at 4°C for later use

[0033] (2) The primary isolated umbilical cord mesenchymal stem cells were seeded in 8 mL of complete medium (DMEM / F12 medium, 5% FBS, 0.5 mg / mL human serum albumin, 5 μg / mL insulin, 50 U / ml penicillin) , the see...

Embodiment 3

[0036] A composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines, comprising the following components by weight: 170 parts of DMEM / F12 medium, 5.5 parts of L-glutamine, 3.8 parts of iron amaranth extract, 10 parts of vitamin C, 5.1 parts of huperzine A and 15 parts of insulin; the extract of Amaranthus amaranthus is an extract in a ratio of 20:1, and the average particle size of the extract is 80-120 mesh.

[0037] The application of the above-mentioned composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines includes the following steps:

[0038] (1) Add L-glutamine, amaranth extract, vitamin C, huperzine A, and insulin to DMEM / F12 medium, mix well to obtain induction medium, store at 4°C for later use

[0039] (2) The primary isolated umbilical cord mesenchymal stem cells were seeded in 8 mL of complete medium (DMEM / F12 medium, 5% FBS, 0.5 mg / mL human serum albumin, 5 μg / mL insulin, 50 U / ml penicillin) , the seeding den...

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Abstract

The invention discloses a composition for inducing umbilical cord mesenchymal stem cells to secrete cell factors. The composition is prepared from the following components in parts by weight: 3.5 to 5.5 parts of L-glutamine, 1.4 to 3.8 parts of acalypha australis extract, 7.5 to 10 parts of vitamin C, 3.2 to 5.1 parts of huperzine A and 10 to 15 parts of insulin. According to the composition provided by the invention, the acalypha australis extract, huperzine A and other components have a synergistic effect, a low-oxygen culture environment is not needed, the cell factor secretion capability of the umbilical cord mesenchymal stem cells is effectively improved, the yield of the cell factors is improved, and a sufficient source is provided for application of the umbilical cord mesenchymal stem cell factors in the clinical or beauty makeup field. The invention further provides specific application of the composition for inducing the umbilical cord mesenchymal stem cells to secrete the cell factors, the induction process is simple and easy to operate, the production cost of the umbilical cord mesenchymal stem cell factors is reduced, and commercial production of the umbilical cord mesenchymal stem cell factors is facilitated.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a composition for inducing umbilical cord mesenchymal stem cells to secrete cytokines and applications thereof. Background technique [0002] Umbilical cord mesenchymal stem cells are a kind of stem cells with abundant sources and easy to be isolated and cultured. Cardiomyocytes, etc., and there is no ethical controversy. Compared with other sources of mesenchymal stem cells, it has obvious advantages in clinical application. At present, umbilical cord mesenchymal stem cells have achieved remarkable results in the field of clinical application. [0003] With the increasing application demand of umbilical cord mesenchymal stem cells, in vitro expansion of umbilical cord mesenchymal stem cells has become a common technical means. During the culture process, technicians found that the culture medium contained a variety of cytokines secreted by umbilical cord mesenchymal stem cells. Facto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12P21/00
CPCC12N5/0668C12P21/00C12N2500/32C12N2500/38C12N2500/76C12N2501/33C12N2500/46
Inventor 岳俊丽陈敏
Owner 岳俊丽
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