Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Medicine for promoting tissue cell regeneration

A technology of tissue regeneration and medicine, which is applied in the field of medicine, can solve the problem of few medicines, and achieve the effect of promoting healing and promoting activation and migration

Pending Publication Date: 2022-06-03
QC BIO-TECH(SHENZHEN) CO LTD
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, the research of scientific researchers has not reached the level of fully understanding the physiological process and principle of tissue and cell regeneration. There are very few drugs on the market that aim at enhancing tissue regeneration, promoting wound healing and recovery of damaged cells.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicine for promoting tissue cell regeneration
  • Medicine for promoting tissue cell regeneration
  • Medicine for promoting tissue cell regeneration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Human skin fibroblasts were suspended in DMEM medium with 10% bovine serum at a cell concentration of 2 × 10 5 / mL suspension, put it into a six-well plate with slides placed (the next step requires slide staining, etc., which cannot be operated in a six-well plate, put the suspension in a six-well plate with slides) cells were grown on slides in six-well plates) and incubated overnight in a 37°C incubator. When the cell conflux rate (confluncy) reaches 50%-70%, use a surgical sterilized blade to scratch hard in the glass slide to cause experimental wounds, such as figure 1 shown.

[0059] 2 mL of PBT with a concentration of 10 μg / mL was used to cover the glass slides scraped from the experimental wounds, sealed for water retention, and cultured in a 37°C incubator; at the same time, the control group was set as a control. The cells were cultured in a ℃ incubator, and the changes in the degree of confluence of fibroblasts in the experimental group and the control grou...

Embodiment 2

[0061] Healing effects of PBT on traumatic wounds in a zebrafish model:

[0062] Prepare 20 adult zebrafish with a weight difference of not more than 50g, and randomly divide them into two groups, each group of 10, named the first group and the second group respectively. The two groups of zebrafish were cultured in two culture tanks with a pH of 7.2 and a water temperature of 27 °C. The caudal fins of the first group of zebrafish were cut about 2-3 mm from the caudal end, and the second group of zebrafish were not treated with caudal fin cutting. The two groups of zebrafish were subdivided into two groups (the first group (1) and the first group (2), the second group (1) and the second group (2)), and placed in a petri dish with a diameter of 35 mm. The first (1) and second (1) groups were co-cultured with fluorescently labeled human neutrophils with PBT at a concentration of 10 mg / mL; the first (2) and second (2) only Cultured with human neutrophils without PBT. The change...

Embodiment 3

[0065] A total of 20 two-week-zero BALB / C mice, half male and half, weighing about 15-20 grams, were randomly divided into two groups (the drug experimental group and the control group, 10 mice each). Dissolve 200 μg of PBT in sterilized 20-30 μL of ethanol, prepare a drug solution, slowly push the drug solution into the tail vein of the mice, observe and record the changes in the behavior, body weight, and body hair of the mice. The body weight of the mice was recorded for 20 days, and it was found that within the effective dose of PBT, compared with the control group, the mice injected with the PBT drug had no significant change in body weight.

[0066] After 20 days, the mice were euthanized, and the brain, heart, gastrointestinal, lung, liver and other organs were removed, and the pathological changes were observed by staining. The pathological pictures of the brain staining of the mice in the drug experimental group and the control group are as follows: Figure 7 As show...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of medicines, and provides a medicine for promoting tissue regeneration. The medicine for promoting tissue regeneration comprises PBT (Polybutylene Terephthalate) or a pharmaceutically acceptable salt of the PBT. The medicine for promoting tissue regeneration, provided by the invention, has the beneficial effects that at an injured part, PBT migrates white blood cells to the injured or infected part and promotes neutrophil to activate and migrate to the injured part in vivo, so that the healing of a matrix wound is obviously stimulated and promoted.

Description

technical field [0001] The application belongs to the technical field of medicine, and specifically relates to a class of medicines for promoting tissue cell regeneration. Background technique [0002] Hepoxilin is a group of substances with epoxy ethanol structure produced by the metabolism of various unsaturated fatty acids. It has both epoxy structure and ethanol residue, which is unstable in vivo. PBT (Hepoxilin StableAnalogs) is a congener of hydroxyepoxide synthesized in vitro. Studies have shown that PBT can inhibit the growth of human cancer cell lines (leukemia (CML), 2 breast cancer lines, prostate cell lines) in cell line models and animal models; antagonize human platelet aggregation in vitro and in rodents. Inhibits the formation of coagulation in vivo. Inhibition of inflammation development in an experimental model of bacterial infection-induced intestinal inflammation. Hydroxyepoxide promotes neutrophil migration and contributes to pain susceptibility by ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/336A61K31/38A61K31/396A61K31/66A61P17/02A61P29/00A61P43/00
CPCA61K31/336A61K31/38A61K31/396A61K31/66A61P17/02A61P29/00A61P43/00
Inventor 温瞳塞西尔·佩斯·阿西亚克皮特·德民
Owner QC BIO-TECH(SHENZHEN) CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com