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Application of urine protein delta homolog 1 and polypeptide fragment thereof in gestational diabetes mellitus

A technology of gestational diabetes and polypeptide fragments, which is applied in the determination/testing of microorganisms, biological testing, disease diagnosis, etc., can solve the problem of decreased expression of DLK1, and achieve the effect of convenient storage

Pending Publication Date: 2022-05-13
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It shows that with the aggravation of glucose metabolism disorder in GDM patients, the expression level of DLK1 decreases, and the ability to improve glucose tolerance and negatively regulate lipogenesis decreases.

Method used

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  • Application of urine protein delta homolog 1 and polypeptide fragment thereof in gestational diabetes mellitus
  • Application of urine protein delta homolog 1 and polypeptide fragment thereof in gestational diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Urine Specimen Collection and Processing

[0020] The clean midstream urine samples of 74 patients with gestational diabetes mellitus (GDM) in the first trimester (8-12 weeks) were collected from the obstetrics clinic and hospitalization of Beijing Shijitan Hospital affiliated to Capital Medical University, aged 24-42 years. Patients with gestational diabetes met the 2011 American Diabetes Association (ADA) diabetes diagnosis and treatment standards, all clinical data from early pregnancy to 42 days postpartum were complete, and 75g sugar OGTT test was performed at 24 to 28 weeks of pregnancy. Without acute and chronic infection, tumor, cardiovascular disease and severe liver and kidney dysfunction, 74 patients with gestational diabetes were divided into GM1 group (FPG5.1%. After the urine was collected, it was centrifuged at 400×g for 5 minutes, the supernatant was taken, aliquoted, and frozen at -80°C. At the same time, in order to track the differences ...

Embodiment 2

[0024] Example 2 Magnetic bead purification and screening of urine peptides

[0025] Weak cation-exchange magnetic beads were used to enrich the peptides in urine, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to analyze the peptide spectrum in urine. After calibrating the instrument with a standard, mix 1 μl of the eluate with 10 μl of matrix (0.3 % α-cyano-4-hydroxycinnamic acid, HCCA), and take 1 μl to spot on the Anchorchip (Autoflex MALDITOF, Bruker-Dalton) target plate on, dry at room temperature. The sample is ionized by nitrogen laser irradiation and then subjected to mass spectrometry analysis, collecting data in the range of 1000-10000 Da, and obtaining a mass spectrogram composed of protein peaks with different mass-to-charge ratios. For each MALDI crystallization site, a total of 400 laser irradiations (50 irradiations at 8 different positions of each crystallization site), the average value represents ...

Embodiment 3

[0026] Example 3 Statistical Analysis

[0027]Pearson correlation analysis was used to correlate the FPG, HbA1c and urine peptide markers of GDM patients, and the two-sided correlation P<0.05 was considered statistically significant. Using the Fisher discriminant function analysis module of SPSS, the independent variables of FPG, HbA1c level, peak urinary peptide biomarkers, neonatal blood glucose (NFPG), neonatal height (NH) and neonatal weight (NW) in GDM patients were established to correlate with Classification discriminant function for GDM glucose metabolism status. The GM1 and GM2, BM1 and BM2 group samples were first classified to clarify the diagnostic performance of the discriminant analysis function model.

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Abstract

The invention provides application of a urine protein delta homolog 1 (DLK1) and a polypeptide fragment thereof, and particularly relates to application of the urine protein delta homolog 1 and the polypeptide fragment thereof in preparation of preparations for diagnosis and treatment of gestational diabetes patients, monitoring and evaluation of blood sugar and metabolic states, mechanism research and the like. Researches prove that the urine protein delta homolog 1 and the polypeptide fragment thereof are reduced along with the increase of the HbA1c level in gestational diabetes patients, have statistical significance in difference between a BM1 group with the HbA1c being less than or equal to 5.1% and a BM2 group with the HbA1c being greater than 5.1%, and can be used for diagnosis and treatment of gestational diabetes patients, monitoring and evaluation of blood sugar and metabolic states, mechanism research and other application detection for various purposes. The advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of a urine sample are exerted, and the protein delta homolog 1 and the polypeptide fragment thereof are detected by utilizing the urine sample.

Description

technical field [0001] The present invention relates to new uses of urinary protein δ homologue 1 and its polypeptide fragments, in particular to the use of urinary protein δ homolog 1 and its polypeptide fragments in diagnosis and treatment of gestational diabetes patients, monitoring, evaluation and mechanism research of blood sugar and metabolic state, etc. Application of preparations. Background technique [0002] The pathogenesis of gestational diabetes mellitus (GDM) is mainly due to the increased glucose demand of pregnant women, insulin resistance and relative insufficient insulin secretion. The incidence of adverse pregnancy outcomes such as miscarriage, premature delivery, cesarean section, and hypertensive disorders in GDM patients with poor blood sugar control is significantly higher than that of normal pregnant women. The incidence of adverse complications such as neonatal hyperbilirubinemia, neonatal respiratory distress syndrome and asphyxia also increased si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543G01N27/62C12Q1/6883
CPCG01N33/6893G01N33/54306G01N33/6848C12Q1/6883C12Q2600/158G01N2800/52G01N2800/042G01N2333/47
Inventor 张曼胡智颖
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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