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Large-scale preparation method of protamine oligopeptide chelated zinc

A technology of protamine and chelated zinc, applied in the field of food and feed, can solve problems that have not yet been found, and achieve the effects of good flavor, improved physical function, and less time consumption

Active Publication Date: 2022-05-13
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hydrolyzate of protamine from the mature testis tissue of aquatic animals has not been found so far—the report of the chelation reaction between protamine peptide and metal zinc to prepare protamine peptide chelated zinc

Method used

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  • Large-scale preparation method of protamine oligopeptide chelated zinc
  • Large-scale preparation method of protamine oligopeptide chelated zinc
  • Large-scale preparation method of protamine oligopeptide chelated zinc

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A large-scale preparation method of protamine oligopeptide chelated zinc, the steps are as follows:

[0048] (1) Raw material pretreatment

[0049] Put the puffer testis into the reaction kettle, add sodium bicarbonate and sodium chloride mixed solution 4 times the weight of the puffer testis, stir for 2 hours, wash with water, wherein the mass volume concentration of the weak alkali solution is 3%, and the concentration of the salt solution is 3%. The mass volume concentration is 5%;

[0050] (2) High-temperature sulfuric acid hydrolysis of testicular nuclear protein

[0051] Put the pretreated and cleaned puffer testis in a reaction kettle, add deionized water twice the weight of the testis, add concentrated sulfuric acid to adjust the pH to 2, and the reaction temperature is 95°C, and the reaction is stirred for 4 hours;

[0052] (3) Adsorption and deodorization and continuous flow centrifugal filtration

[0053] After the hydrolysis reaction, lower the temperatur...

Embodiment 2

[0061] A large-scale preparation method of protamine oligopeptide chelated zinc, the steps are as follows:

[0062] (1) Raw material pretreatment

[0063] Put the silver carp testis into the reaction kettle, add potassium bicarbonate and sodium chloride mixed solution twice the weight of the silver carp testis, stir for 3 hours, wash with water, wherein the mass volume concentration of the weak alkali solution is 4%, and the salt solution The mass volume concentration is 4%;

[0064] (2) High-temperature sulfuric acid hydrolysis of testicular nuclear protein

[0065] Put the pretreated and cleaned silver carp testis in a reaction kettle, add deionized water of 1 times the testis weight, add concentrated sulfuric acid to adjust the pH to 2.5, the reaction temperature is 100°C, and stir for 3 hours;

[0066] (3) Adsorption and deodorization and continuous flow centrifugal filtration

[0067] After the hydrolysis reaction, lower the temperature to 45°C, first add calcium hydro...

Embodiment 3

[0075] A large-scale preparation method of protamine oligopeptide chelated zinc, the steps are as follows:

[0076] (1) Raw material pretreatment

[0077] Put the squid testis into the reaction kettle, add the mixed solution of sodium bicarbonate and potassium chloride 1 times the weight of the squid testis, stir for 1 hour, wash with water, wherein the mass volume concentration of the weak alkali solution is 2%, and the mass volume of the salt solution The concentration is 3%;

[0078] (2) High-temperature sulfuric acid hydrolysis of testicular nuclear protein

[0079] Put the pretreated and cleaned squid testis in a reaction kettle, add deionized water 0.5 times the testis weight, add concentrated sulfuric acid to adjust the pH to 3, the reaction temperature is 90 ° C, and stir for 4 hours;

[0080] (3) Adsorption and deodorization and continuous flow centrifugal filtration

[0081] After the hydrolysis reaction, lower the temperature to 45°C, first add calcium hydroxide to...

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Abstract

The invention relates to a large-scale preparation method of protamine oligopeptide chelated zinc. The method comprises the following steps: by taking mature testis tissues of aquatic organisms as raw materials, hydrolyzing testis nucleoprotein by adopting high-temperature sulfuric acid, carrying out continuous flow centrifugal filtration and multi-stage membrane separation to obtain a protamine oligopeptide solution which has the content of more than or equal to 95%, does not have nucleotide and has high metal zinc chelating activity, then adding zinc salt, stirring, carrying out constant-temperature chelating, and carrying out nanofiltration membrane desalination and impurity removal, thereby obtaining the protamine oligopeptide with high metal zinc chelating activity. The particle size of the nano protamine oligopeptide chelated zinc particles is less than 300nm, and most (more than 80%) molecular weights of the nano protamine oligopeptide chelated zinc particles are less than 1000Da. Although a chemical extraction and metal chelation method is adopted, compared with a commonly used enzymolysis extraction and metal chelation method, the protamine oligopeptide chelated zinc obtained by the method disclosed by the invention is higher in yield, less in time consumption and higher in chelation rate. Meanwhile, the production of large-scale preparation of the protamine oligopeptide chelated zinc does not need special instruments and equipment, the technological method is simple and reliable, the technological process is efficient and flexible, and the protamine oligopeptide chelated zinc subjected to adsorption deodorization and embedding deodorization is good in flavor in the aspects of smell and taste, is easier to serve as a food additive or a nutritional supplement, and is suitable for industrial production. The product is accepted and eaten by vast consumers; and the protamine oligopeptide chelated zinc which is not subjected to fishy smell removal treatment can still be used as a feed additive.

Description

technical field [0001] The invention relates to a large-scale preparation method of protamine oligopeptide chelated zinc that can be used as a zinc supplement for food or feed, relates to chemical extraction and metal chelation technology, and belongs to the field of food and feed. Background technique [0002] Zinc is an essential trace element in human and animal systems, known as the "element of life". It not only has important biological functions, but also participates in the synthesis and composition of various enzymes, and is closely related to the metabolism of the body and the occurrence of certain diseases. Zinc deficiency in the body will lead to negative problems such as decreased human immunity, decreased male reproductive function, growth retardation in children, decreased appetite and anorexia in infants, and central nervous system dysfunction. [0003] At present, zinc supplements for human or animal bodies are usually inorganic zinc such as zinc oxide, zinc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C07K1/107C07K1/34C07K1/14A23L33/165A23L33/18A23L5/20A23P10/30A23K20/147A23K20/20
CPCC07K14/461A23L33/165A23L33/18A23L5/273A23P10/30A23K20/147A23K20/30A23V2002/00A23V2200/30A23V2250/55A23V2250/1642A23V2250/5114A23V2250/5432Y02A20/131
Inventor 陈思谨洪专张怡评郭洪辉谢全灵陈晖陈伟珠
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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