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Method for enhancing anaerobic biodegradation of halogenated phenol by static magnetic field

An anaerobic biology and static magnetic field technology, applied in chemical instruments and methods, anaerobic digestion treatment, biological water/sewage treatment, etc., to achieve stable properties, reduce treatment costs, and long service life

Pending Publication Date: 2022-05-13
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, magnetic fields may also have negative effects on organisms. Quan et al. explored the effect of static magnetic fields on the removal of trichlorethylene in biofilters. When the magnetic field strength was 130mT, the removal of trichlorethylene was greatly inhibited (Quan Y, Wu H, Yin Z, Fang Y and Yin C.Effect of static magneticfield on trichloroethylene removal in a biotrickling filter[J].BioresourTechnol,2017,239:7-16)

Method used

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  • Method for enhancing anaerobic biodegradation of halogenated phenol by static magnetic field
  • Method for enhancing anaerobic biodegradation of halogenated phenol by static magnetic field
  • Method for enhancing anaerobic biodegradation of halogenated phenol by static magnetic field

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Effect test

Embodiment 1

[0030] (1) Sludge domestication: The simulated wastewater was exposed to nitrogen for 15 minutes before use to exhaust the residual oxygen in the wastewater to an anaerobic state. Add the initial simulated wastewater containing 4-BP concentration of 5 mg / L into a 125 mL anaerobic bottle, and add anaerobic sludge with a sludge concentration of 2.47 g / L, and seal the anaerobic bottle. After the 4-BP in the simulated wastewater was completely degraded, the concentration of 4-BP in the wastewater was gradually increased to 50 mg / L, and the sludge domestication was completed after the effluent quality was stable.

[0031] (2) Construct a static magnetic field-anaerobic biological system: Add domesticated sludge into the magnetic field, set the magnetic field strength to 1.25mT, 11.65mT, 21.25mT, 30.41mT and 40.1mT respectively, and pass through the For 50mg / L simulated wastewater, acclimated for 10 days, and constructed a static magnetic field-anaerobic biological system.

[0032]...

Embodiment 2

[0037] According to the experimental data of embodiment 1, analyze experiment, select optimal magnetic field strength to be 21.25mT, other conditions are identical with embodiment 1, different salt concentrations (NaCl concentration are respectively 0, 15, 20 and 25g / L) are set, explore Effects of static magnetic field on anaerobic organisms under complex water quality conditions.

[0038] The result is as figure 2 As shown, with the increase of salt concentration, the removal rate of anaerobic organisms to 4-BP is gradually decreasing. When the NaCl concentration is 0g / L and the magnetic field strength is 0mT, the removal rate of 50mg / L 4-BP within 5h It can reach more than 60%; when the salinity increases to 20g / L, the removal rate of 50mg / L 4-BP is only 37.05±2.76%. In contrast, under the action of a static magnetic field of 21.25mT, when the NaCl concentration is 0g / L, the removal rate of 4-BP to 50mg / L has reached more than 95.0% in 5h; 15g / L NaCl and 21.25mT Static ma...

Embodiment 3

[0040] According to the experimental data of embodiment 1, analyze experiment, select optimum magnetic field strength 21.25mT, other conditions are the same as embodiment 1, research the long-term influence of magnetic field to microorganism, carry out by a definite date 20 days continuous acclimatization degradation experiment, explore static magnetic field to microorganism Secretion of bromophenol dehalogenase and effects of EPS.

[0041] The result is as image 3 As shown, the activity of bromophenol dehalogenase increases with the increase of the magnetic exposure time. Compared with the non-magnetic field exposure on the first day of magnetic exposure, the activity increases by 3.79%. The increase is relatively slow. With the increase of the magnetic exposure time, the magnetic exposure The activity of bromophenol dehalogenase increased by 28.41%, 28.51% and 42.69% respectively at 5d, 10d and 20d. With the increase of magnetic exposure time, the content of EPS also incre...

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Abstract

The invention discloses a method for enhancing anaerobic biodegradation of halogenated phenol by a static magnetic field. The method comprises the following steps: adding simulated wastewater with halogenated phenol concentration of 10-50mg / L into anaerobic sludge to complete sludge domestication; placing the domesticated anaerobic sludge in a magnetic field to construct a static magnetic field coupling anaerobic biological system; adding the halogenated phenol wastewater into a static magnetic field coupling anaerobic biological system, and degrading halogenated phenol. A low-intensity static magnetic field is introduced into an anaerobic microorganism system, so that extracellular polymeric substances and enzyme activity of bacteria can be effectively promoted, the capacity of anaerobic organisms for resisting external complex environment fluctuation is improved, the long-term stability of an anaerobic biological system is promoted, and the removal rate of halogenated phenol in wastewater can reach 98% or above under the optimal condition. The method disclosed by the invention is low in cost, efficient and simple, and the wastewater treatment cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of industrial wastewater treatment containing halogenated phenols, and relates to a method for strengthening anaerobic biodegradation of halogenated phenols by a static magnetic field. Background technique [0002] The halogenated phenols in phenolic compounds have an "electron-withdrawing" effect due to the halogenated group on the benzene ring, which can enhance the molecular polarity of the phenolic compound, and the p electrons of the halogen atoms and the π electrons on the benzene ring can form Very stable conjugated system, easy to combine with the enzyme system in the organism, and produce long-term chronic toxic effects on the organism. Halogenated phenols, especially bromophenols, are widely present in wastewater discharges from industries such as fuel, paint, photography, pesticides, flame retardants and wood preservatives, and are carcinogenic, teratogenic and mutagenic. It is difficult to degra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/28C02F3/34C02F101/36
CPCC02F3/28C02F3/342C02F2101/36
Inventor 陈丹朱奕朋沈锦优江心白刘晓东
Owner NANJING UNIV OF SCI & TECH
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