Bionic biological glue as well as preparation method and application thereof

A bio-glue and bio-polymer technology, applied in the field of biomimetic bio-glue and its preparation, can solve problems such as difficulty in matching irregular shapes, inability to bond with wet surfaces, difficult tissue or wound repair, etc., and achieve improved hemostasis performance and preparation method. Green, easy-to-operate effect

Active Publication Date: 2022-05-03
WUQI BIOMEDICAL TECH (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing bioglues have the following problems: (1) toxicity; (2) inability to bond to wet surfaces; (3) weak adhesion; (4) difficult to match irregular shapes of defects, etc.
For example, super glue (cyanoacrylic adhesive) has strong adhesion to tissue, but it is difficult to use in tissue or wound repair due to its strong cytotoxicity; wet surface is also the main cause of adhesion failure, due to human body In the presence of body fluids, it is crucial to find bioglues that can also adhere in a liquid environment; weak adhesion often leads to excessive friction on the tissue bonding surface, resulting in inflammation or directly causing the adhesive to fall off. Ultimately lead to bond failure; other new synthetic gels, although highly adhesive to tissue, are difficult to use clinically due to the presence of toxic initiators and activators

Method used

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  • Bionic biological glue as well as preparation method and application thereof
  • Bionic biological glue as well as preparation method and application thereof
  • Bionic biological glue as well as preparation method and application thereof

Examples

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preparation example Construction

[0041] Another aspect of the embodiments of the present invention also provides the aforementioned method for preparing biomimetic bioglue, which includes:

[0042] Reacting the first mixed reaction system comprising dopamine hydrochloride, amino-containing biopolymers, NHS, EDC, trifluoroacetic acid and / or acetic acid to prepare the dopamine-modified amino-containing biopolymers;

[0043] The second mixed reaction system comprising p-benzaldehyde, hydroxyl-containing biopolymer, 2,2-dimethylolpropionic acid, dicyclohexylcarbodiimide and tetrahydrofuran is reacted in a closed environment to obtain the said aldylation Hydroxy-containing biopolymers;

[0044] And, mix the dopamine-modified amino-containing biopolymers, hydroxyl-containing biopolymers, and aldylated hydroxyl-containing biopolymers uniformly, then add metal chloride salts and mix them uniformly with a solvent, and then stand and incubate for 10- After 48 hours, the biomimetic bioglue is produced by spontaneous biom...

Embodiment 1

[0075]Weigh 500mg of collagen and dissolve it in 50mL of 0.5wt% trifluoroacetic acid, stir at room temperature for 2-3 hours until completely dissolved to form a collagen solution, then add 0.5g of dopamine hydrochloride to the collagen solution, and stir to dissolve under airtight conditions , while adjusting the pH value of the mixed solution to 5.5 by adding 1mol / L sodium hydroxide solution dropwise; solution to adjust the pH value of the mixed solution to 5.5, also weigh 1.15g of EDC and dissolve it in 15mL deionized water to form an EDC solution, and adjust the pH value of the mixed solution to 5.5 by adding dropwise 10wt% trifluoroacetic acid solution; The prepared NHS and EDC solutions were added to the mixed solution at an interval of 15 minutes. Afterwards, the entire reaction system was placed in a closed environment and reacted at room temperature for 20 hours. After 20 hours, the reaction solution was transferred to a dialysis bag, and then placed Dialyze in 4mM hy...

Embodiment 2

[0080] Take chitosan 1000mg and dissolve in 50mL 0.5wt% trifluoroacetic acid, stir at room temperature for 1-2 hours to completely dissolve to form a chitosan solution, then add 0.6g dopamine hydrochloride in the chitosan solution, and Stir and dissolve under airtight conditions, and at the same time adjust the pH value of the mixed solution to 5.5 by adding 1mol / L sodium hydroxide solution dropwise; then weigh 0.85g of NHS and dissolve it in 15mL deionized water to form an NHS solution 1mol / L sodium hydroxide solution to adjust the pH value of the mixed solution to 5.5, also weigh 1.15g of EDC and dissolve it in 15mL deionized water to form an EDC solution, and adjust the pH of the mixed solution by adding 10wt% trifluoroacetic acid solution dropwise value to 5.5; the prepared NHS and EDC solutions were added to the mixed solution in sequence, and the interval time between additions was 15min. After that, the entire reaction system was placed in a closed environment and reacte...

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Abstract

The invention discloses bionic biological glue as well as a preparation method and application thereof. The biomimetic biological glue comprises the following components: dopamine modified amino-containing biopolymer, hydroxyl-containing biopolymer, formylated hydroxyl-containing biopolymer, chlorinated metal salt and a solvent. The bionic biogel disclosed by the invention has excellent biocompatibility, electrical conductivity, wet surface adhesion capacity, injectability, self-healing property, biodegradability, hemostasis and healing promotion capacity; meanwhile, network enhancement and adhesion enhancement are achieved through spontaneous biomineralization of metal cations in the bionic biological glue, the hemostasis performance of the material is remarkably improved through introduction of biomineralized metal particles, and the biomineralized metal particles cooperate with dopamine-modified amino-containing biomacromolecules and hydroxyl-containing biomacromolecules together for rapid hemostasis; therefore, the bionic organism has a wide application prospect in the fields of biomedicine and tissue engineering.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a biomimetic bioglue and its preparation method and application. Background technique [0002] Recently, bioglue has been considered as a promising material category due to its gradual application in biomedical devices such as tissue adhesion, wound repair, tissue repair, rapid hemostasis, drug delivery, and cosmetic filling. However, the existing bioglues have the following problems: (1) toxicity; (2) inability to bond to wet surfaces; (3) weak adhesion; (4) difficult to match irregular shapes of defects, etc. For example, super glue (cyanoacrylic adhesive) has strong adhesion to tissue, but it is difficult to use in tissue or wound repair due to its strong cytotoxicity; wet surface is also the main cause of adhesion failure, due to human body In the presence of body fluids, it is crucial to find bioglues that can also adhere in a liquid environment; weak adhes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/50A61L27/56A61L26/00A61L24/04A61L24/02
CPCA61L27/56A61L27/50A61L27/446A61L26/0085A61L26/0095A61L26/0061A61L24/0036A61L24/0089A61L24/001A61L2300/418A61L2400/06A61L2400/04
Inventor 戴建武陈艳艳杨文
Owner WUQI BIOMEDICAL TECH (JIANGSU) CO LTD
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