Preparation method and application of multifunctional pea peptide

A pea peptide and multifunctional technology, which is applied in the field of preparation of multifunctional pea peptides, can solve the problems that the research on pea peptides has not yet been reported, and achieves the effects of strong production schedule flexibility, low production cost and high qualification rate.

Pending Publication Date: 2022-04-26
琛蓝(美国)营养制品股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing reports on the preparation of pea peptides, enzymatic hydrolysis is mainly used, but the research on the combination of fermentation and enzymatic hydrolysis to prepare pea peptides has not been reported yet.

Method used

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  • Preparation method and application of multifunctional pea peptide
  • Preparation method and application of multifunctional pea peptide
  • Preparation method and application of multifunctional pea peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A preparation method of a multifunctional pea peptide, comprising the following steps:

[0055] S1. Configure pea protein feed solution:

[0056] The pea protein powder (with a dry basis protein content of 83.20%) was added to pure water at a mass ratio of 1:10, and stirred for 30 minutes to obtain a pea protein feed solution.

[0057] S2. Preparation of complex protease:

[0058] ①Fermentation: Inoculate Bacillus natto into the liquid fermenter containing pea protein feed liquid, the inoculation amount is 2%, adjust the pH to 7.0, the temperature to 37°C, the speed of the fermenter to be 200r / min, and the air flow to be 20L / min. The pressure of the tank was controlled to be 0.03 MPa, and the fermentation broth was obtained after culturing for 40 hours. The fermentation broth was centrifuged by a 16000 r / min tubular centrifuge to obtain a composite protease liquid, which was freeze-dried to obtain a composite protease.

[0059] ② Compounding: Compounding the compound ...

Embodiment 2

[0072] A preparation method of a multifunctional pea peptide, comprising the following steps:

[0073] S1. Configure pea protein feed solution:

[0074] The pea protein powder (with a dry basis protein content of 83.20%) was added to pure water at a mass ratio of 1:10, and stirred for 30 minutes to obtain a pea protein feed solution.

[0075] S2. Preparation of complex protease:

[0076] ①Fermentation: Inoculate Bacillus natto into the liquid fermenter containing pea protein feed liquid, the inoculation amount is 3%, adjust the pH to 7.0, the temperature to 37°C, the speed of the fermenter to be 250r / min, and the air flow to be 20L / min. The pressure of the control tank was 0.03 MPa, and the fermentation broth was obtained after culturing for 36 hours. The fermentation broth was centrifuged by a tubular centrifuge at 16000 r / min to obtain a composite protease liquid, which was freeze-dried to obtain a composite protease.

[0077] ② Compounding: Compounding the compound protea...

Embodiment 3

[0087] A preparation method of a multifunctional pea peptide, comprising the following steps:

[0088] S1. Configure pea protein feed solution:

[0089] The pea protein powder (with a dry basis protein content of 83.20%) was added to pure water at a mass ratio of 1:15, and stirred for 30 minutes to obtain a pea protein feed solution.

[0090] S2. Preparation of complex protease:

[0091] ①Fermentation: Inoculate Bacillus natto into a liquid fermenter containing pea protein feed liquid, the inoculum amount is 2%, adjust the pH to 7.0, the temperature to 37°C, the speed of the fermenter to be 200r / min, and the air flow to be 25L / min. The pressure of the tank was controlled to be 0.03 MPa, and the fermentation broth was obtained after culturing for 40 hours. The fermentation broth was centrifuged by a 16000 r / min tubular centrifuge to obtain a composite protease liquid, which was freeze-dried to obtain a composite protease.

[0092] ② Compounding: Compounding the compound prote...

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Abstract

The invention discloses a preparation method and application of multifunctional pea peptide, and belongs to the technical field of biological medicines.The preparation method of the multifunctional pea peptide comprises the following steps that S1, pea protein feed liquid is prepared; s2, preparation of compound protease: 1) fermentation: inoculating the pea protein material liquid with bacillus natto for fermentation, centrifuging the fermentation liquid, taking the supernatant, and drying to obtain the compound protease; 2) compounding: compounding the compound protease with papain, alkaline protease and flavourzyme to prepare compound protease; s3, performing enzymolysis; s4, performing membrane filtration; and S5, drying. The method is simple in process, short in period, high in yield and low in cost, the prepared pea peptide is high in yield and protein recovery rate, the protein hydrolysate is small in relative molecular mass, the product flavor is good, and the pea peptide product has blood pressure reduction, uric acid reduction and antioxidant activity and is a high-quality pea peptide product.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method and application of a multifunctional pea peptide. Background technique [0002] Peas are leguminous plants of the genus Pea, also known as snow peas, wheat beans, bi bean, etc., rich in starch, protein, vitamins, minerals and various amino acids necessary for the human body. Pea protein is an excellent vegetable protein, and its essential amino acid composition is close to the recommended model of FAO / WHO, with low allergenicity and high nutritional value, and has received widespread attention. [0003] However, due to the poor solubility and digestibility of protein, pea protein cannot fully exert its biological activity in the human body. Therefore, it is necessary to treat pea protein by certain means to improve its physiological activity and maximize the utilization of pea Nutritional value of protein. [0004] At present, the deep utili...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A61K38/01A61P9/12A61P19/06A61P39/06
CPCC12P21/06C07K1/34A61K38/011A61P9/12A61P19/06A61P39/06
Inventor 邹圣灿曹廷锋樊芳刘廷廷王楠王家庭刘金丽王尚龙
Owner 琛蓝(美国)营养制品股份有限公司
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