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Fructus cannabis functional polypeptide and preparation method thereof

A hemp seed and multi-functional technology, which is applied in the field of hemp seed functional polypeptide and its preparation, can solve the undisclosed problems of inhibiting the activity of α-amylase and lipase, and achieve the improvement of low utilization rate of raw materials, low utilization rate of raw materials, and the like. The effect of increasing yield and reducing processing equipment

Active Publication Date: 2022-04-22
厦门元之道生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Most of the hemp seed polypeptides prepared by the existing technology are only related to the effect of lowering blood pressure, and have not been disclosed to have the effect of inhibiting the activity of α-amylase and lipase

Method used

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  • Fructus cannabis functional polypeptide and preparation method thereof
  • Fructus cannabis functional polypeptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] S100. Hemp seed cake pretreatment: Take 1000 grams of defatted hemp seed meal powder crushed to 80-100 mesh (containing 70% protein), add 20 kg of water, stir well, boil for 4 minutes, pour into a colloid mill and circulate for grinding for 3 minutes , forming the hemp seed meal liquid;

[0034] S200, the first enzymatic hydrolysis: use hydrochloric acid solution to adjust the pH of the hemp seed meal liquid to 2.6; add 3.4310 acid protease 3.2 million u, hydrolyze at 45°C for 130min; after the hydrolysis, inactivate the enzyme at 80°C for 8min to obtain the first enzymatic solution;

[0035] S300, the second enzymatic hydrolysis: After the first enzymatic hydrolysis liquid is cooled, add 2.8 million u of pepsin, and hydrolyze it for 50 minutes under the conditions of pH 2.2 and 37°C; Obtain the second enzymolysis solution;

[0036] S400, sediment enzymolysis: centrifuge the second enzymolysis solution at 4000rpm for 10min with a centrifuge to obtain supernatant A and...

Embodiment 2

[0045] S100. Pretreatment of hemp seed meal: take 100 grams of defatted hemp seed meal powder crushed to 80-100 mesh (containing 70% protein), add 2 kg of water, stir well, boil for 3 minutes, pour into a colloid mill and circulate for grinding for 3 minutes , forming the hemp seed meal feed liquid.

[0046] S200, the first enzymatic hydrolysis: adjust the pH of the hemp seed meal liquid to 3.0 with citric acid solution, add 3.4310 acid protease 350,000 u, hydrolyze at 48°C for 90 minutes; after the hydrolysis is completed, inactivate the enzyme at 90°C 5min to obtain the first enzymatic hydrolysis solution.

[0047] S300, the second enzymatic hydrolysis: after the first enzymatic hydrolysis solution is cooled, add 245,000 u of pepsin, and hydrolyze it for 45 minutes at a pH of 2.5 and 40°C; after the hydrolysis, heat to 85°C to inactivate the enzyme for 3 minutes Obtain the second enzymolysis solution.

[0048] S400, sediment enzymolysis: centrifuge the second enzymolysis l...

Embodiment 3

[0057] S100. Hemp seed cake pretreatment: Take 100 grams of defatted hemp seed meal powder crushed to 80-100 mesh (containing 70% protein), add 2 kg of water, stir well, boil for 3 minutes, pour into a colloid mill and circulate for grinding for 3 minutes , forming the hemp seed meal liquid;

[0058] S200, the first enzymatic hydrolysis: use hydrochloric acid solution to adjust the pH of the hemp seed meal liquid to 3.2; add 3.4310 acid protease 420,000 u, and hydrolyze at 52°C for 70 minutes; after the hydrolysis, extinguish the enzyme at 85°C for 8 minutes to obtain the first One enzymatic solution;

[0059] S300, the second enzymatic hydrolysis: After the first enzymatic hydrolysis solution is cooled, add 180,000 u of pepsin, and hydrolyze it for 30 minutes at pH 2.8 and 42°C; after hydrolysis, heat to 85°C to inactivate the enzyme for 3 minutes, Obtain the second enzymolysis solution;

[0060] S400, sediment enzymatic hydrolysis: centrifuge the second enzymatic hydrolysi...

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Abstract

The invention relates to the field of fructus cannabis processing, in particular to fructus cannabis functional polypeptide and a preparation method of the fructus cannabis functional polypeptide. The preparation method of the fructus cannabis functional polypeptide comprises the steps that firstly, defatted fructus cannabis meal and water form fructus cannabis meal liquid; hydrolyzing the fructus cannabis meal material liquid through acid protease and pepsin in sequence, and centrifuging to obtain supernate A and precipitate; the fructus cannabis functional polypeptide is prepared by the following steps: dissolving fructus cannabis in water to obtain a precipitate, hydrolyzing the precipitate with neutral protease, centrifuging to obtain a supernatant B, mixing the supernatant A and the supernatant B, intercepting 300-6000 Da filtrate through membrane filtration, and concentrating to obtain the fructus cannabis functional polypeptide. The preparation method comprises the following steps: sequentially hydrolyzing with acid protease and pepsin to obtain polypeptide capable of inhibiting the activity of alpha-amylase; neutral protease is adopted to hydrolyze residues obtained after first two times of enzymolysis to obtain polypeptides capable of inhibiting lipase activity, the two polypeptides are mixed to have inhibitory activity of two digestive enzymes, and the digestibility of starch and fat substances in intestinal tracts can be effectively reduced.

Description

technical field [0001] The invention relates to the field of hemp seed processing, in particular to a hemp seed functional polypeptide and a preparation method thereof. Background technique [0002] Hemp (scientific name: Cannabissativa), also known as hemp, hemp, red hemp, etc., is an annual herb of the genus Cannabis in the family Moraceae, widely distributed in temperate and tropical regions of Asia and Europe. Ripe hemp seeds provide excellent oil, fiber and protein. [0003] The defatted hemp seed meal is a cake obtained by extracting edible hemp seed oil by pressing and leaching, and the protein content is 50% to 70%. At home and abroad, there have been reports of hydrolyzing hemp seed protein with alkaline protease, neutral protease, papain and trypsin to prepare polypeptides. For example, the patent document with the announcement number CN108070629B and the announcement date of February 12, 2021 discloses a method for the industrial production of hemp protein oligo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34
CPCC12P21/06C07K1/14C07K1/34
Inventor 赵大洲王常青曹秀娟徐梅
Owner 厦门元之道生物科技有限公司
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