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Anaerobic complex microbial inoculant for degrading COD (Chemical Oxygen Demand) in wastewater and application of anaerobic complex microbial inoculant

A compound bacterial agent and oxygen compound technology, applied in the field of environmental microorganisms, can solve the problems of the original process cost of secondary pollution, low COD degradation efficiency, substandard effluent COD, etc., achieving good COD degradation effect, improving processing capacity and impact resistance. capacity, the effect of improving the settling performance

Pending Publication Date: 2022-04-22
青岛蔚蓝赛德生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ordinary biological treatment of sewage and wastewater has problems such as low COD degradation efficiency, effluent COD not up to standard, slow system start-up speed, poor impact resistance, easy to produce secondary pollution, high original process cost, and complex process.

Method used

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  • Anaerobic complex microbial inoculant for degrading COD (Chemical Oxygen Demand) in wastewater and application of anaerobic complex microbial inoculant
  • Anaerobic complex microbial inoculant for degrading COD (Chemical Oxygen Demand) in wastewater and application of anaerobic complex microbial inoculant
  • Anaerobic complex microbial inoculant for degrading COD (Chemical Oxygen Demand) in wastewater and application of anaerobic complex microbial inoculant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Screening and isolation of Brevundimonas mediterranei COD-Y-1

[0040] (1) Screening and isolation of bacterial strains

[0041] The sewage from the anaerobic pool of the biochemical section of an industrial sewage treatment station in Feixian County, Linyi City was collected, and the sewage was diluted to 10 by gradient dilution method. -2 、10 -3 and 10 -4 100 μl of each dilution was drawn into the separation medium (peptone 10g, beef powder 3g, sodium chloride 5g, agar powder 15g, tap water 1000mL), coated evenly and then inverted in an anaerobic tank, cultured at 30°C, About 72h to grow a single colony. Single colonies with different shapes were selected and transferred to the test tube slant separation medium, placed in an anaerobic tank at 30°C for about 72 hours, and then transferred to a 4°C refrigerator for storage for later use.

[0042] A total of 4 strains were obtained according to the above isolation method, numbered respectively: COD-Y-1, CO...

Embodiment 2

[0051] Example 2: Detection and identification of Brevundimonas mediterranei COD-Y-1

[0052] 1. Experimental method

[0053] 1.1 Extraction of bacterial genomic DNA

[0054] (1) Use a 2ml centrifuge tube to collect 1.0×10 9 (1ml bacterial solution OD600 is 1-1.5) bacterial culture, centrifuge at 12,000×g for 30s, and discard the supernatant. Suspend the pellet with 150 μl Buffer S to which RNase A has been added.

[0055] (2) Add 20 μl of lysozyme stock solution, mix well, and let stand at room temperature for 5 minutes.

[0056] (3) Add 30 μl of 0.25mol / L EDTA (pH 8.0), mix well, and ice-bath for 5 minutes.

[0057] (4) Add 450 μl Buffer G-A, vortex for 15 seconds, and bathe in water at 65° C. for 10 minutes.

[0058] (5) Add 400μl Buffer G-B and 1ml Buffer DV (pre-cooled at 4°C), mix vigorously, and centrifuge at 12,000×g for 2min.

[0059] (6) Discard the upper phase as much as possible, and keep the interphase precipitate and the lower phase. Add 1ml of 4°C pre-coo...

Embodiment 3

[0096] Embodiment 3: the effect evaluation of the different proportions of each strain in the bacterial agent formula

[0097] 3.1 Degradation COD evaluation experiment:

[0098]Each bacterial powder in the bacterial agent formula was evaluated according to different proportions (mass ratio), and the live bacteria amount of Bacillus cereus bacterial powder was (100-120)×10 8 CFU / g, the amount of live bacteria of Brevundimonas mediterranea powder is (70-90)×10 8 CFU / g;

[0099] Separately pack 100mL of biochemical papermaking wastewater into 100mL anaerobic bottles, sterilize at 115°C for 30 minutes, and then cool to room temperature. Under aseptic conditions, 200 ppm of solid bacterial agents compounded by Bacillus cereus and Brevundimonas mediterranei in different proportions were added to it, and then placed at 30°C for static culture, and the waste water was detected every 2 days. COD content. Three parallel experiments were set up for each experimental group, and a bla...

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Abstract

The invention relates to an anaerobic complex microbial inoculant for degrading COD (Chemical Oxygen Demand) in sewage and wastewater, the anaerobic complex microbial inoculant comprises bacillus cereus and brevundimonas mediterranei, the viable count ratio of the bacillus cereus to the brevundimonas mediterranei is 1: 4-7: 1, the brevundimonas mediterranei is preserved in China General Microbiological Culture Collection Center, the preservation number of the brevundimonas mediterranei is CGMCC (China General Microbiological Culture Collection Center), and the preservation number of the brevundimonas mediterranei is CGMCC NO. The collection number is CGMCC (China General Microbiological Culture Collection Center) No.24126. After the complex microbial inoculant is used, the starting speed of an anaerobic system can be effectively increased, the treatment capacity and impact resistance of the system can be improved, the biodegradability of a water body can be improved, the proportion of organic pollutants which are difficult to degrade can be reduced, the output of residual sludge can be reduced, and the sludge settling performance can be improved.

Description

technical field [0001] The invention relates to a composite microbial bacterial agent, in particular to a composite microbial bacterial agent capable of degrading COD in industrial sewage and wastewater under anaerobic conditions, and belongs to the technical field of environmental microorganisms. Background technique [0002] my country is a big industrial country, and the discharge of industrial wastewater is huge. The sources of industrial wastewater in my country mainly include industries such as papermaking, coking, pharmaceuticals, and textile printing and dyeing. Most of the wastewater generated by these industries is high-concentration and refractory organic wastewater, which has high chemical oxygen demand (COD), refractory, complex water quality and toxicity. great features. At present, the harmlessness and recycling of industrial wastewater has become a key link to solve the problems of environmental pollution and water shortage. [0003] The main methods for tre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C12R1/01
CPCC12N1/20C02F3/34C02F2209/08
Inventor 吴娜刘圣鹏
Owner 青岛蔚蓝赛德生物科技有限公司
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