Kit for determining trimethylamine oxide as well as preparation method and application of kit

Abstract

According to the kit for determining trimethylamine oxide and the preparation method and application of the kit, the current situation that a kit prepared through a solution technology is poor in stability can be effectively solved, and freeze-drying and long-term stability of an internal standard substance can be achieved. The kit comprises a series of trimethylamine oxide calibrators and a deuterated trimethylamine oxide internal standard substance, the series of trimethylamine oxide calibrators are freeze-dried substances of a series of trimethylamine oxide calibrator working solutions with concentration gradient, and the series of trimethylamine oxide calibrator working solutions are trimethylamine oxide calibrator working solutions with concentration gradient formed by diluting a trimethylamine oxide standard solution with known concentration with a matrix solution; the deuterated trimethylamine oxide internal standard substance is a lyophilized substance of a deuterated trimethylamine oxide internal standard substance working solution with a predetermined concentration, wherein the lyophilized substance is formed by diluting a deuterated trimethylamine oxide standard substance solution with a known concentration by using a matrix solution; a matrix in the matrix solution is serum albumin.

Description

Technical field [0001] The present invention relates to the field of detection technology for endogenous biomarkers, in particular to a kit for determining trimethylamine oxide and preparation methods and applications thereof. Background [0002] As an endogenous biomarker, trimethylamine oxide (TMAO), like other biomarkers, needs to first address matrix interference to ensure quantitative accuracy. Therefore, when choosing the right substrate, developers often take two main approaches: [0003] 1) Deduct the zero concentration blank blood matrix, that is, select the mixed blood as the matrix, subtract the different concentrations of calibrators and control products from the zero concentration mixed blood matrix, remove the TMAO interference in the mixed blood matrix itself, and then perform curve fitting and sample content determination. Although this practice ensures the "original taste" of the matrix, it ignores the fact that there are significant differences in TMAO content ...

AI Technical Summary

Problems solved by technology

Although this approach ensures the “original taste” of the matrix, it ignores the fact that there are significant differences in TMAO content among samples, and the fact that mixed matrixes also have batch-to-batch inconsistencies, coupled with the fact that the source of mixed human blood matrix and ethics are restricted. Many limitations, so it is difficult to apply to the industrialization of the development of in vitro diagnostic kits (IVD)

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