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Preparation method of hydrogel kit for monitoring degradation of organophosphorus pesticide

An organophosphorus pesticide and hydrogel technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve problems such as limited application, false positive results, interference, etc., to simplify the sensing process, improve stability, and improve detection sensitivity Effect

Pending Publication Date: 2022-04-19
JILIN UNIV
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Problems solved by technology

In addition, the brightness displayed by monochromatic fluorescent indicators is susceptible to background interference from plant pigments and secondary metabolites, and eye-based visual quantification is limited to semi-quantitative analysis, which easily leads to false positive results, and accurate measurement still relies on complex instruments , which greatly limits its application in POC monitoring

Method used

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  • Preparation method of hydrogel kit for monitoring degradation of organophosphorus pesticide
  • Preparation method of hydrogel kit for monitoring degradation of organophosphorus pesticide
  • Preparation method of hydrogel kit for monitoring degradation of organophosphorus pesticide

Examples

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Effect test

Embodiment 1

[0026] Example 1: AuNCs@MnO 2 Preparation and Characterization of Fluorescent Composite Materials

[0027] First, AuNCs were prepared as nanomaterials with BSA as a template, and AuNCs@MnO were synthesized by a co-template method. 2 fluorescent composite material, the MnCl 2 Solution (50mmol L -1 , 100μL), AuNCs solution (1.0mL) and sodium hydroxide solution (1.0molL -1 , 100 μL) were mixed and stirred for 3 hours, then purified with a dialysis bag, and dialyzed for 1 day to remove small molecules.

[0028] The result is as figure 1 As shown, the AuNCs@MnO 2 The morphology and structure of the composite were characterized, and lattice fringes of AuNCs with a size of 0.216 nm were observed, indicating that AuNCs had been successfully anchored in MnO 2 Surface ( figure 1 B). According to the EDX images, it can be observed that Au and Mn exist in AuNCs@MnO 2 in fluorescent composites (Figure 1C). Because MnO 2 The role of nanosheets makes AuNCs@MnO 2 The ζ-potential v...

Embodiment 2

[0029] Example 2: The ratiometric fluorescent hydrogel kit is used for the detection of paraoxon

[0030] First, in order to realize the POC test, the AuNCs@MnO 2 The fluorescent composite was immobilized in a three-dimensional hydrogel carrier, and a test kit was prepared. First, the SA solution (20 mg mL -1 , 100μL), ATCh (2mmol L -1 , 10μL) and AuNCs@MnO 2 Fluorescent composite (2.5 μg mL -1 , 20 μL) was added to the 96-well plate, after mixing thoroughly, the Ca(NO 3 ) 2 Solution (10.0mg mL -1 , 30 μL) was added to the above solution to prepare a hydrogel.

[0031] The hydrogel production process is as follows figure 2 As shown in A, the well-formed SA hydrogel has no fluorescent background under UV light and remains fixed when the tube is inverted ( figure 2 B), and the introduction of AuNCs@MnO into the hydrogel 2 After fluorescent composite, red emission fluorescence was observed at the bottom of the tube. figure 2 The SEM image in C shows the 3D fiber-lik...

Embodiment 3

[0035] Embodiment 3: the detection of paraoxon in the actual sample

[0036] In order to verify based on AuNCs@MnO 2 The accuracy of the composite ratio-type hydrogel kit is used to detect the spiked recovery of paraoxon in actual samples such as tap water, Songhua River water, orange juice, and milk. Add different concentrations (0.025, 0.1 and 0.25μg mL -1 ) of the paraoxon standard solution, the standard recovery of paraoxon was measured between 98.49%~104%, and the relative standard deviation (RSD) of different real samples was between 1.17%~8.78% ( Figure 5 A). It was shown that the ratiometric fluorescent hydrogel kit combined with Image J software is practical and reliable in the POC detection of paraoxon residues.

[0037] Carrying out a ratiometric fluorescent hydrogel kit on the degradation of organophosphorus pesticides in Chinese cabbage, calibration data linear fitting: FL550 / FL 650 =0.375+0.670Log[paraoxon]ng mL -1 (R 2 =0.998)( Figure 5 B). The hydrog...

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Abstract

The invention discloses a preparation method of a hydrogel kit for monitoring degradation of organophosphorus pesticides. The fluorescent composite material is embedded into a sodium alginate hydrogel system in a microwell plate to serve as a signal indicator, and the gel kit is prepared. A ratio fluorescence method is constructed by utilizing the fluorescence characteristic of AuNCs in the composite material and the oxidase-like activity of MnO2 nanosheets on OPD. According to the present invention, with the combination of the oxidation reduction reaction of the AChE enzymatic product TCh and the MnO2 nanosheet and the efficient inhibition effect of the pesticide on AChE, the organophosphorus pesticide degradation timely monitoring (POC) hydrogel kit is ingeniously constructed, and the color information is converted into the pesticide concentration information by using the smart phone software so as to be used for the accurate monitoring of the oxygen and phosphorus residue and degradation in the Chinese cabbage. According to the ratio type hydrogel kit developed by the invention, the sensing process is simplified, the sensing efficiency and sensitivity are greatly improved, a new method is created for organophosphorus pesticide detection, and a technical support is provided for application of a POC technology in food safety monitoring.

Description

technical field [0001] The invention belongs to the field of biosensor technology and food safety detection, in particular to a fluorescent composite material anchored by gold nanoclusters to manganese dioxide nanosheets (AuNCs@MnO 2 NSs), the synergistic application of fluorescence properties of AuNCs and MnO in composite materials 2 The enzyme-like properties of NSs catalyze the oxidation of o-phenylenediamine to construct a ratiometric fluorescence method, which reduces background interference through built-in fluorescence signal correction. Combined with acetylcholinesterase (Acetyl cholinesterase, AChE) to catalyze thioacetylcholine (thioacetylcholine, ATCh) to generate thiocholine (TCh) and MnO 2 The oxidation-reduction reaction between NSs and the high-efficiency inhibitory effect of organophosphorus pesticides on AChE, cleverly constructed a hydrogel kit for timely monitoring of organophosphorus pesticide degradation (POC), which was applied to the accurate monitor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 李红霞邹睿琦孙春燕吕婷刘妮苏长顺张铁华
Owner JILIN UNIV
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