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Molecular marker for identifying pig aggressiveness, detection method and application

A molecular marker and aggressive technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problem of lack of SNP molecular markers of aggressiveness in pigs, etc., to improve pork quality and Consumer satisfaction, reduced meat quality deterioration, and the effect of speeding up the breeding process

Active Publication Date: 2022-04-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still a lack of SNP molecular markers that can be directly used to detect the aggressiveness of pigs

Method used

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  • Molecular marker for identifying pig aggressiveness, detection method and application
  • Molecular marker for identifying pig aggressiveness, detection method and application
  • Molecular marker for identifying pig aggressiveness, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Screening of molecular genetic markers related to pig aggressive behavior.

[0029] In order to detect gene mutations related to aggressive behavior, pigs with stronger aggressive behavior and weaker aggressive behavior were randomly selected for DNA sequencing, and genes in multiple pathways such as serotonin pathway, dopamine pathway, and axon guidance pathway were analyzed. The upstream and downstream sequences are analyzed, the specific method is as follows:

[0030] 1. Collect ear samples:

[0031] Take 2-10 mg of fresh Suhuai pig ear tissue, and extract its DNA by phenol-chloroform extraction method;

[0032] 2. Polymorphism detection of related gene loci:

[0033] In order to find the gene mutations related to pig aggressive behavior, 24 pigs were randomly selected for whole genome resequencing.

[0034] After genome-wide resequencing analysis and screening, it was found that a SNP in ARHGAP24 gene might be associated with pig aggressive behavior. T...

Embodiment 2

[0041] Example 2 Using the SNPs genotype of the ARHGAP24 gene to predict the aggressiveness of pigs

[0042] In the group of experimental pigs, 200 pigs with aggressive behavior records were randomly selected for ARHGAP24 gene sequencing, and the SNP was genotyped.

[0043] The amplification primers used for SNPs typing are as follows:

[0044] Forward primer F: 5'-ATCACCAGGAAGAAAGAC-3' (SEQ ID NO.2);

[0045] Reverse primer R: 5'-AGAGAAATTCAAAGCATG-3' (SEQ ID NO.3).

[0046] The results are shown in Table 2. There are three genotypes of GG, AG, and AA, and there are extremely significant differences in the distribution of pig aggressiveness groups.

[0047] Table 2 Gene frequency and allele frequency analysis of pig ARHGAP24 gene single nucleotide polymorphism (SNP):

[0048]

[0049] Since the occurrence of aggressive behavior of pigs is related to the mother's parity at birth, its own sex, the pen it is in, and the quality of its mixed precursors, a generalized linear m...

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Abstract

The invention discloses a molecular marker for identifying pig aggressiveness, a detection method and application. According to the invention, the SNP obviously associated with the attack behavior of the pig is found for the first time, and the SNP is the SNP with the polymorphism G / A at the 133243042bp of the No.8 chromosome of the reference sequence of the Sscrofa11.1 version of the pig genome. The SNP obviously associated with the attack behavior of the pig can be used as a molecular marker for detecting the aggressiveness of the pig and breeding a pig group with relatively low aggressiveness, and has important guiding significance for improving the pork quality and animal welfare. The method provided by the invention is simple to operate, can automatically detect on a machine only by obtaining the genome of the ear sample or the blood sample of the individual to obtain the genotype of the individual so as to analyze the aggressiveness of the individual, and is low in cost, high in accuracy, time-saving and labor-saving, and suitable for popularization and application.

Description

technical field [0001] The invention relates to the application fields of molecular biology and biological analysis, in particular to a molecular marker for identifying the aggressiveness of pigs, a detection method and an application. Background technique [0002] Aggressive behavior of pigs after mixed groups can lead to body surface damage, slow growth, poor meat quality, and transient immunity reduction caused by aggressive stress, which affects the healthy development of the pig industry and is also an important animal welfare issue . In recent years, people have realized that it is necessary to strengthen the selection of pig aggressive behavior traits in pig breeding, but most of the research on pig aggressive behavior remains at identification and judgment, nutritional regulation, physiological and biochemical markers, etc. There are few reports on the analysis of the mechanism. [0003] With the development of molecular biology techniques and quantitative genetics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 周波徐庆磊
Owner NANJING AGRICULTURAL UNIVERSITY
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