Exosome purification method and device

A purification method and purification device technology, applied in the biological field, can solve the problems of high cost, long duration, complicated purification process, etc., and achieve the effect of solving insufficient production capacity, meeting market demand, high commercial value and promotion value

Pending Publication Date: 2022-04-12
宁波甬恒瑶瑶智能科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is an exosome purification method to solve the problems of cumbersome purification process, long duration and high cost of conventional exosome purification methods currently on the market

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  • Exosome purification method and device
  • Exosome purification method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0029] This example provides specific methods for exosome purification:

[0030] S1 Secondary tangential ultrafiltration: pass the extract containing exosomes into the first-stage tangential flow ultrafiltration device, and pass through the first-stage ultrafiltration membrane to recover the target liquid after ultrafiltration to obtain permeate , to trap impurities; then the permeate will continue to flow through the second-stage tangential flow ultrafiltration device, and the ultrafiltration membrane pore size of the second-stage tangential flow ultrafiltration device is smaller than that of the exosomes diameter, it is intercepted, and recovered to obtain the enriched solution;

[0031] The pore diameter of the first-stage TFF ultrafiltration membrane is equal to 0.20 μm, and the pore diameter of the second-stage TFF ultrafiltration membrane is equal to 0.01 μm;

[0032] S2 enrichment solution treatment: The buffer mobile phase is vacuum filtered or ultrasonically degassed...

Embodiment 2

[0037] This example provides specific methods for exosome purification:

[0038] S1 Secondary tangential ultrafiltration: pass the extract containing exosomes into the first-stage tangential flow ultrafiltration device, and pass through the first-stage ultrafiltration membrane to recover the target liquid after ultrafiltration to obtain permeate , to trap impurities; then the permeate will continue to flow through the second-stage tangential flow ultrafiltration device, and the ultrafiltration membrane pore size of the second-stage tangential flow ultrafiltration device is larger than that of the exosomes , intercepting it, and recovering the obtained enrichment solution;

[0039]The pore diameter of the first-stage TFF ultrafiltration membrane is equal to 0.19 μm, and the pore diameter of the second-stage TFF ultrafiltration membrane is equal to 0.02 μm;

[0040] S2 enrichment solution treatment: The buffer mobile phase is vacuum filtered or ultrasonically degassed for 15 mi...

Embodiment 3

[0045] This example provides specific methods for exosome purification:

[0046] S1 Secondary tangential ultrafiltration: pass the extract containing exosomes into the first-stage tangential flow ultrafiltration device, and pass through the first-stage ultrafiltration membrane to recover the target liquid after ultrafiltration to obtain permeate , to trap impurities; then the permeate will continue to flow through the second-stage tangential flow ultrafiltration device, and the ultrafiltration membrane pore size of the second-stage tangential flow ultrafiltration device is smaller than that of the exosomes Diameter, it is intercepted, and the enriched solution obtained is recovered;

[0047] The pore diameter of the first-stage TFF ultrafiltration membrane is equal to 0.18 μm, and the pore diameter of the second-stage TFF ultrafiltration membrane is equal to 0.03 μm;

[0048] S2 enrichment solution treatment: The buffer mobile phase is vacuum filtered or ultrasonically degass...

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Abstract

The invention provides an exosome purification method which comprises the steps of S1, secondary two-stage tangential flow ultrafiltration, S2, ultrafiltrate treatment and S3, freeze-drying treatment.The exosome product prepared through the method is high in purity, and the preparation method is simple; the invention further provides an exosome purification device, and the exosome purification device and method can be used for separation and purification production of high-purity, high-flux and large-scale factory samples and have high commercial value and popularization value.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for purifying exosomes and a device thereof. Background technique [0002] In 2013, the Nobel Prize in Physiology or Medicine was awarded to three scientists who studied the regulatory mechanism of cell vesicle transport. After that, exosome research gradually became a scientific research hotspot. Exosomes are small vesicles with a diameter of about 30-150nm secreted by living cells. They have a typical lipid bilayer structure and are widely found in cell culture supernatant, blood, saliva, urine, and semen. , amniotic fluid and other biological fluids. It carries a variety of important information such as proteins, lipids, and RNA, and plays an important role in the transmission of substances and information between cells. It can regulate cell metabolism, cell apoptosis, and immune regulation. It can be used as a medical beauty, regeneration Medicine, disease diag...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/12C12M1/00C12N5/00
Inventor 何加铭李思杨茗予涂建飞
Owner 宁波甬恒瑶瑶智能科技有限公司
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