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Gadolinium-doped gold nanoparticles, biomimetic synthesis method and application of gadolinium-doped gold nanoparticles in stem cells

A gold nanoparticle, biomimetic synthesis technology, applied in the field of nanomaterials, can solve the problems of no therapeutic function, single function, unable to achieve stem cell targeted photothermal therapy at the same time, etc., achieving good photothermal effect, simple operation, repeatability strong effect

Pending Publication Date: 2022-04-12
JIANGSU UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the defect of this existing technology is that the function is relatively single, only dual-mode imaging of magnetic resonance and fluorescence can be realized, and it has no therapeutic function, and it is impossible to realize the targeted photothermal therapy of stem cells at the same time.

Method used

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  • Gadolinium-doped gold nanoparticles, biomimetic synthesis method and application of gadolinium-doped gold nanoparticles in stem cells
  • Gadolinium-doped gold nanoparticles, biomimetic synthesis method and application of gadolinium-doped gold nanoparticles in stem cells
  • Gadolinium-doped gold nanoparticles, biomimetic synthesis method and application of gadolinium-doped gold nanoparticles in stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Weigh silk fibroin powder and dissolve it in double-distilled water to prepare a silk fibroin solution with a concentration of 25 mg / mL, add it to the reaction bottle, turn on the magnetic stirring, and add 5 mL of 20 mmol / L chloroauric acid solution;

[0034] (2) Add sodium hydroxide solution to make the pH value 9-10;

[0035] (3) Ultrasonic for 1 min, add 80 μL of 29.9 mmol / L gadolinium chloride solution;

[0036] (4) Continue to react with magnetic stirring for 8-12 hours at normal temperature;

[0037] (5) Centrifuge (12000rpm, 10min), and wash with water three times to obtain gadolinium-doped gold nanoparticles.

[0038] Such as figure 1 As shown in the transmission electron microscope images of the prepared gadolinium-doped gold nanoparticles, the particle size of the obtained gadolinium-doped gold nanoparticles is about 500 nm, the shape is petal-shaped, and the distribution is relatively uniform.

Embodiment 2

[0040] (1) Weigh 1 g of chloroauric acid tetrahydrate and dissolve it in 10 mL of double distilled water, add 40 μL to the reaction bottle, and add 5 mL of 3.125 mg / mL silk fibroin solution;

[0041] (2) adding sodium hydroxide solution to adjust the pH value to 9-10;

[0042] (3) After ultrasonication for 3 minutes, add 80 μL of 30 mmol / L gadolinium chloride solution;

[0043] (4) Magnetic stirring at normal temperature, continuous reaction for 8-12h;

[0044] (5) centrifuge (12000rpm, 10min), and wash with water three times to obtain gadolinium-doped gold nanoparticles.

[0045] Such as figure 2 As shown, the transmission electron microscope image of the prepared gadolinium-doped gold nanoparticles shows that the obtained gadolinium-doped gold nanoparticles have a particle size of about 50nm, a spherical shape, and an obvious crystal lattice can be observed.

Embodiment 3

[0047] (1) dry and quantify the biomimetic nanoparticles synthesized in Example 1 or 2, and configure an aqueous solution whose concentration of nanoparticles is 1 mg / mL;

[0048] (2) Take 100-200 μL of the above solution and put it in an EP tube, use 808nm near-infrared laser at 0.5-3W / cm 2 The laser power is irradiated for 3-6min;

[0049] (3) Monitor the temperature change of the solution in real time with an infrared imager. An obvious temperature rise can be found after laser irradiation, which indicates that the synthesized biomimetic nanoparticles have photothermal conversion ability.

[0050] The result is as image 3 and 4 As shown, the obtained gadolinium-doped gold nanoparticles have a characteristic absorption peak at 535nm, which is the characteristic absorption peak of gold nanoparticles, and also has a wider near-infrared absorption, which also has an absorption value at 808nm; the nanoparticles After 4 minutes of 808nm laser irradiation, the temperature incre...

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Abstract

The invention discloses gadolinium-doped gold nanoparticles, a biomimetic synthesis method and application of the gadolinium-doped gold nanoparticles in stem cells, the method adopts silk fibroin as a reducing agent and a stabilizing agent, Au < 3 + > is fixed on the silk fibroin by utilizing rich amino acid residues of the silk fibroin, the Au < 3 + > is reduced into Au < + >, then the pH value is adjusted to 9-10, and the Au < + > is further reduced into Au. And adding a gadolinium chloride solution to prepare the gadolinium-doped gold nanoparticles. The method has the following advantages: (1) the reaction conditions of the method are green and mild, the operation is simple, the consumed time is short, and the repeatability is strong; (2) according to the method, silk fibroin is used as a reducing agent and a stabilizing agent for the first time, and chloroauric acid reduction, stabilization and gadolinium ion doping are completed in a reaction system; and (3) the gold nanoparticles prepared by the method and the stem cells marked by the gold nanoparticles have good photothermal effect, biocompatibility and MR / CT dual-mode imaging capability, so that the gold nanoparticles and the stem cells marked by the gold nanoparticles have wide prospects in the aspects of stem cell imaging tracing and treatment.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and relates to a new synthesis method of gold nanoparticles, specifically gadolinium-doped gold nanoparticles, a bionic synthesis method and an application in stem cells. Background technique [0002] Gold nanostructures have been widely studied in the field of tumor diagnosis and treatment because of their superior stability and biocompatibility. Gold nanoparticles have a surface plasmon resonance effect, which can convert the absorbed laser light into heat, and thus be used for photothermal therapy of tumors. In addition to good photothermal therapy effects, gold nanoparticles also have high X-ray attenuation coefficient and photoacoustic imaging characteristics, and are popular research objects for CT imaging and photoacoustic imaging. In recent years, there have been diagnosis and treatment systems that chelate other ions (such as Gd, Pt) or load drugs (DOX, ICG) on gold nanostructures....

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/054A61K41/00A61K47/64A61K49/04A61K49/14A61K49/18A61P35/00B82Y5/00B82Y15/00B82Y30/00
Inventor 杨传雪乐文俊胡益辉吴琼英
Owner JIANGSU UNIV OF SCI & TECH
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