Gadolinium-doped gold nanoparticles, biomimetic synthesis method and application of gadolinium-doped gold nanoparticles in stem cells
A gold nanoparticle, biomimetic synthesis technology, applied in the field of nanomaterials, can solve the problems of no therapeutic function, single function, unable to achieve stem cell targeted photothermal therapy at the same time, etc., achieving good photothermal effect, simple operation, repeatability strong effect
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Embodiment 1
[0033] (1) Weigh silk fibroin powder and dissolve it in double-distilled water to prepare a silk fibroin solution with a concentration of 25 mg / mL, add it to the reaction bottle, turn on the magnetic stirring, and add 5 mL of 20 mmol / L chloroauric acid solution;
[0034] (2) Add sodium hydroxide solution to make the pH value 9-10;
[0035] (3) Ultrasonic for 1 min, add 80 μL of 29.9 mmol / L gadolinium chloride solution;
[0036] (4) Continue to react with magnetic stirring for 8-12 hours at normal temperature;
[0037] (5) Centrifuge (12000rpm, 10min), and wash with water three times to obtain gadolinium-doped gold nanoparticles.
[0038] Such as figure 1 As shown in the transmission electron microscope images of the prepared gadolinium-doped gold nanoparticles, the particle size of the obtained gadolinium-doped gold nanoparticles is about 500 nm, the shape is petal-shaped, and the distribution is relatively uniform.
Embodiment 2
[0040] (1) Weigh 1 g of chloroauric acid tetrahydrate and dissolve it in 10 mL of double distilled water, add 40 μL to the reaction bottle, and add 5 mL of 3.125 mg / mL silk fibroin solution;
[0041] (2) adding sodium hydroxide solution to adjust the pH value to 9-10;
[0042] (3) After ultrasonication for 3 minutes, add 80 μL of 30 mmol / L gadolinium chloride solution;
[0043] (4) Magnetic stirring at normal temperature, continuous reaction for 8-12h;
[0044] (5) centrifuge (12000rpm, 10min), and wash with water three times to obtain gadolinium-doped gold nanoparticles.
[0045] Such as figure 2 As shown, the transmission electron microscope image of the prepared gadolinium-doped gold nanoparticles shows that the obtained gadolinium-doped gold nanoparticles have a particle size of about 50nm, a spherical shape, and an obvious crystal lattice can be observed.
Embodiment 3
[0047] (1) dry and quantify the biomimetic nanoparticles synthesized in Example 1 or 2, and configure an aqueous solution whose concentration of nanoparticles is 1 mg / mL;
[0048] (2) Take 100-200 μL of the above solution and put it in an EP tube, use 808nm near-infrared laser at 0.5-3W / cm 2 The laser power is irradiated for 3-6min;
[0049] (3) Monitor the temperature change of the solution in real time with an infrared imager. An obvious temperature rise can be found after laser irradiation, which indicates that the synthesized biomimetic nanoparticles have photothermal conversion ability.
[0050] The result is as image 3 and 4 As shown, the obtained gadolinium-doped gold nanoparticles have a characteristic absorption peak at 535nm, which is the characteristic absorption peak of gold nanoparticles, and also has a wider near-infrared absorption, which also has an absorption value at 808nm; the nanoparticles After 4 minutes of 808nm laser irradiation, the temperature incre...
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