Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ABHD2 gene expression inhibitor and application and medicine thereof

A technology of gene expression and inhibitors, applied in the field of ABHD2 gene expression inhibitors, can solve the problems that have yet to be revealed, and achieve the effect of enriching molecular mechanisms, promoting the development process, and promoting the apoptosis of liver cancer cells

Active Publication Date: 2022-04-12
XINXIANG MEDICAL UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the potential role of TDP-43 in the development of liver cancer and related molecular mechanisms still remain to be revealed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ABHD2 gene expression inhibitor and application and medicine thereof
  • ABHD2 gene expression inhibitor and application and medicine thereof
  • ABHD2 gene expression inhibitor and application and medicine thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Materials and methods

[0029] 1.1 Cell culture

[0030] Human liver cancer cells SMMC-7721, MHCC97H, Huh-7 and HEK293T cell lines were purchased from Suzhou Beina Chuanglian Biotechnology Co., Ltd. Cultured in a 37°C incubator under carbon dioxide concentration.

[0031] 1.2 Plasmid construction

[0032] Using the mRNA extracted from SMMC-7721 cells as a template, the coding region sequence (CDS) of TDP-43 was amplified by RT-PCR, and the enzyme cutting sites XhoI and BamHI were introduced during the amplification, and then the sequence and the vector pEGFP-C1 were respectively Carry out double enzyme digestion, use T4 DNA ligase to connect the PCR products of the above enzyme digestion with the pEGFP-C1 vector, and finally construct the recombinant plasmid pEGFP-TDP-43 for the exogenous overexpression of TDP-43. Using the same method, the ABHD2 CDS was cloned into the pCMV3 vector to construct pCMV3-ABHD2 for ABHD2 overexpression. The 3'UTR of ABHD2 was amplifi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of biological medicine, in particular to an ABHD2 gene expression inhibitor, application thereof and a medicine. The inhibitor is one or two of 1) and 2): 1) a product which is prepared or screened by taking the ABHD2 gene as a target and has an inhibition effect on the ABHD2 gene; and 2) a product which is obtained by taking the upstream regulatory gene of the ABHD2 gene as a target or screening and has an inhibition effect on the upstream regulatory gene. The result of the invention shows that the TDP-43 can be combined with the ABHD2 mRNA 3 'UTR and enhance the stability of the ABHD2 mRNA, so that the expression of the ABHD2 is up-regulated. According to the application, the TDP-43 affects the lipid metabolism process through the ABHD2, then liver cancer cell apoptosis is inhibited, the development process of liver cancer is finally promoted, liver cancer cell apoptosis can be promoted and liver cancer cell proliferation can be inhibited by down-regulating expression of the ABHD2 or the TDP-43, and the result has important theoretical guidance significance and clinical application value for precise treatment of liver cancer.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an ABHD2 gene expression inhibitor, its application and medicine. Background technique [0002] For many years, primary hepatocellular carcinoma (hereafter referred to as liver cancer) has been endangering human life and health, and is one of the most common malignant tumors in the world. The complex pathogenesis of HCC involves multiple processes such as chronic inflammation, fibrosis, and oncogene dysregulation. There are various treatments for liver cancer, depending on the stage of the cancer and the condition of the patient. Liver cancer can be treated mainly by means of liver transplantation, hepatectomy, radiofrequency ablation, transcatheter arterial chemoembolization, and targeted therapy. However, so far, the five-year survival rate of liver cancer patients is still low, with only one-third of patients living an average of more than 60 months after surgical resection, trans...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/7088A61P35/00
Inventor 刘博雯曹金玲王相允申志发陈露露周佳王伊蕾
Owner XINXIANG MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com