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Application of Pythium oligandrum fermentation mycoplasma in antitumor

A technology of Pythium oligandrum and fermentation bacteria, which is applied in the medical field

Inactive Publication Date: 2016-04-27
INNER MONGOLIA UNIV FOR THE NATITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, studies on tumor suppression have not been reported

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  • Application of Pythium oligandrum fermentation mycoplasma in antitumor
  • Application of Pythium oligandrum fermentation mycoplasma in antitumor
  • Application of Pythium oligandrum fermentation mycoplasma in antitumor

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Embodiment Construction

[0021] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0022] In the embodiment of the present invention, the application of Pythium oligandrum fermented bacteria in anti-tumor is studied from the following aspects:

[0023] 1. Preparation of Pythium oligandrum fermentation bacteria:

[0024] Bacteria: Pythium oligandrum

[0025] Source: Soil of Hanshan National Nature Reserve, Tongliao City, Inner Mongolia

[0026] 1. Isolation and preservation of Pythium oligandrum

[0027] 1.1 Preservation of Pythium oligandrum strains

[...

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Abstract

The invention discloses an application of a Pythium oligandrum fermentation mycoplasma in antitumor. A preparation method of the Pythium oligandrum fermentation mycoplasma comprises the following steps: carrying out separate preservation on Pythium oligandrum strains; collecting soil containing the Pythium oligandrum strains, preserving the collected soil at 4DEG C, taking 0.1-1mg of soil particles, placing the soil particles in a 9cm culture dish, slowly pouring a Pythium oligandrum selective medium, carrying out dark culturing at 25DEG C, picking marginal mycelia after a bacterium colony appears, transferring the picked marginal mycelia to the fresh Pythium oligandrum selective medium, purifying, and preserving the strains; and preparing the Pythium oligandrum selective fermentation mycoplasma: punching activated Pythium oligandrum blocks with the medium by using a disinfection puncher, putting the punched blocks in a conical flask provided with a PDA liquid medium, and culturing the blocks at 25DEG C under 130r / min until the conical flask is full of block spheres to obtain the Pythium oligandrum fermentation mycoplasma. The Pythium oligandrum fermentation mycoplasma has antitumor activity, and especially a specific concentration of the Pythium oligandrum fermentation mycoplasma has an obvious tumor cell apoptosis causing effect.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to the application of Pythium oligandrum fermentation bacteria in anti-tumor aspect. Background technique [0002] At present, in anti-tumor research, inducing apoptosis of cancer cells is an important way. Promoting tumor cell apoptosis can achieve the purpose of tumor shrinkage and cancer regression. For example, traditional anti-tumor drugs cisplatin and melphalan can all Stimulates the mitochondrial apoptotic pathway in cells, triggering apoptosis. This method has obvious advantages over the treatment method of killing tumor cells, because if anti-tumor drugs can induce the apoptosis of tumor cells in large quantities and activate their own programmed death process, it will avoid the release of tumor cells due to a large number of necrotic cells. Various side effects caused by intracellular substances can reduce the damage of chemotherapy to normal cells and prolong the surviv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61P35/00C12R1/645
CPCC12N1/14
Inventor 银玲王思珍田迅李依韦赵汝冀照君
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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