Pharmaceutical composition containing PI3K inhibitor and application of pharmaceutical composition
A composition and inhibitor technology, applied in the field of biomedicine, to achieve the effects of improved curative effect, significant therapeutic effect, and low toxicity
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Embodiment 1
[0026] Example 1, the effect of LY294002 acting alone on the migration of esophageal cancer cells
[0027] Esophageal cancer cell lines KYSE30 and KYSE450 were inoculated into 96-well plates at the number of 12,000 cells per well. After the cells adhered to the wall, they were synchronized for 12 hours, scratched with a scratch instrument, washed twice with PBS, and replaced with 2% Fetal bovine serum medium, take pictures, this time is 0h, then add the blank control group and the LY294002 experimental group with doses of 0, 30, 15, 7.5, 3.25 μM respectively, take pictures after 24 hours of culture, measure the width, and calculate the migration Rate. According to cell migration ratio=width (experimental group 0h-experimental group 24h) / width (blank group 0h-blank group 24h)ⅹ100%, the results are detailed in figure 1 and Table 1.
[0028] Table 1 The results of each cell migration rate measurement
[0029]
[0030] From Table 1 and figure 1 It can be seen that LY294002 ...
Embodiment 2
[0031] Example 2, the combined effect of LY294002 and SNX-2112 on tumor cells and normal cells
[0032] KYSE30 esophageal cancer cell line, ECA-109 esophageal cancer cell line, cisplatin-resistant ECA-109 esophageal cancer cell line, A549 lung cancer cell line, HepG2 liver cancer cell line and HUVEC normal vascular endothelial cells were used at 3000-6000 cells per well The number was inoculated into 96-well plates, and after the cells adhered to the wall, the cells were divided into control group, LY294002 single drug group, SNX-2112 single drug group and LY294002 and SNX-2112 drug combination group; after 48 hours of culture, each well was added 10 μL of CCK-8 solution, continue to culture for 1 h, read the OD value (wavelength 450 nm) on a microplate reader, read the absorbance value of each well, and calculate the cell survival rate. According to the formula of cell survival rate (%)=(OD experimental group-OD blank) / (OD control group-OD blank)×100%, calculate the cell surv...
Embodiment 3
[0034] Example 3. The combined effect of LY294002 and SNX-2112 on the migration of drug-resistant esophageal cancer cells
[0035] Human cisplatin-resistant ECA-109 esophageal cancer cells were inoculated into a 96-well plate at a rate of 12,000 cells per well. After the cells adhered to the wall, they were synchronized for 12 hours, scratched with a scratch instrument, and washed twice with PBS. Change the medium containing 2% fetal bovine serum and take pictures. At 0h, divide the cells into control group, LY294002 single-drug group, SNX-2112 single-drug group and LY294002 and SNX-2112 drug combination group. culture medium or drug solution, take pictures after 24 hours of culture, measure the width, and calculate the mobility. According to cell migration ratio=width (experimental group 0h-experimental group 24h) / width (blank group 0h-blank group 24h)×100%, the results are detailed in image 3 .
[0036] From image 3 It can be seen that, compared with the control group and...
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