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Application of syringic acid in preparation of medicine for treating osteoarthritis caused by cartilage degradation

A technology for osteoarthritis and cartilage degradation, applied in the direction of drug combinations, antipyretics, anti-inflammatory agents, etc., can solve the problems of inherent mechanical and biochemical properties, easy to break, etc., achieve low immunogenicity, easy synthesis, Mitigate the effect of progression

Pending Publication Date: 2022-04-12
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although methods such as joint debridement, bone marrow stimulation, and cartilage transplantation (autologous or homologous) have been used clinically to assist cartilage repair, the cartilage tissue repaired by these methods tends to develop into fibrocartilage or hyaline cartilage-like. The biochemical characteristics cannot be compared with normal hyaline cartilage, and it is easy to break and promote the formation of secondary osteoarthritis; therefore, the development of effective, reliable and safe drugs that can promote cartilage repair has always been a hot and difficult issue in the treatment of arthritis

Method used

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  • Application of syringic acid in preparation of medicine for treating osteoarthritis caused by cartilage degradation
  • Application of syringic acid in preparation of medicine for treating osteoarthritis caused by cartilage degradation
  • Application of syringic acid in preparation of medicine for treating osteoarthritis caused by cartilage degradation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example provides the ability of syringic acid to promote the expression of type II collagen and aggrecan in chondrocytes in vitro;

[0031] Including the following steps:

[0032] Step 1, in vitro isolation and extraction of primary mouse articular chondrocytes for culture;

[0033] Step 2, intervene chondrocytes with different concentrations of syringic acid;

[0034] Step 3, using CCK8 method to observe its toxic and side effects on chondrocytes. The results of CCK8 as figure 1 shown.

[0035] Specifically, it mainly includes: cell seeding plate, first intervening cells with 50uM syringic acid for 24 hours, extracting total RNA, and performing fluorescent quantitative PCR to detect the effect of syringic acid on chondrocyte type 2 collagen (COL2) and aggrecan (aggrecan; ACAN) expression;

[0036] Intervene chondrocytes with lipopolysaccharide 1ug / ml or lipopolysaccharide plus 50uM syringic acid, extract total RNA, and perform fluorescent quantitative PCR to ...

Embodiment 2

[0039] This example provides the ability of syringic acid to improve the microenvironment in vivo and reduce the progression of osteoarthritis in mice;

[0040] Including the following steps:

[0041] Twenty C57BL / 6 female mice were randomly divided into sham operation group (sham), model group (DMM), low-dose SA group (SA-L) and high-dose SA group (SA-H), 5 in each group, A mouse osteoarthritis model was established in vivo, and the medial meniscus (DMM) of the right mouse knee joint was resected. After operation, the mice in the DMM+low-dose SA group and the DMM+high-dose SA group were treated with 0.3mg / kg and 1.5mg / kg respectively. Carry out intra-articular injection, once every three days, and the other groups are given equal volume of PBS, and the mice are sacrificed after 4 weeks;

[0042] The above four groups (sham, DMM, SA-L, SA-H) were subjected to safranin fast green and toluidine blue fast green histological staining analysis, the results are as follows image 3...

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Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to application of syringic acid in preparation of a medicine for treating osteoarthritis caused by cartilage degradation. The syringic acid (3, 5-dimethoxy-4-hydroxybenzoic acid) can promote expression of cartilage cell type II collagen and aggregation proteoglycan in vitro, can relieve the progress of osteoarthritis caused by meniscus resection in vivo, relieves articular cartilage abrasion, has a protective effect on articular cartilage, and can be used for preparing a medicine for treating osteoarthritis. The method has important significance for treating or preventing osteoarthritis caused by cartilage degradation.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to the application of syringic acid in the preparation of medicines for treating osteoarthritis caused by cartilage degradation. Background technique [0002] Arthritis (OA) is a chronic degenerative disease characterized by articular cartilage degeneration, secondary osteophyte formation, subchondral bone sclerosis, and synovial inflammation. The most common site of arthritis; with the acceleration of the aging process in the world, knee osteoarthritis has become a high-incidence disease that plagues human beings. Knee osteoarthritis is one of the main causes of disability in the world. According to epidemiological data, the United States There are more than 14 million people with knee osteoarthritis, and in China, the incidence rate of knee osteoarthritis among people aged 55-64 has reached 40%, and the incidence rate of women is higher than that of men; knee...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61P19/02A61P29/00
Inventor 徐亮亮王敏吴思落张雅歌丁伶莉周冰玉
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU UNIV OF CHINESE MEDICINE
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