Method for preventing and controlling pear ring spot by using dopamine

A technology of pear ring spot and dopamine, which is applied in the field of fruit tree disease prevention and control, to achieve the effects of convenient use, cost saving, and reduction of pesticide use

Pending Publication Date: 2022-04-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There are no reports on the effect of dopamine on plant biotic stress capacity

Method used

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  • Method for preventing and controlling pear ring spot by using dopamine
  • Method for preventing and controlling pear ring spot by using dopamine
  • Method for preventing and controlling pear ring spot by using dopamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Dopamine under different concentrations of embodiment 1 prevents and controls pear ring spot

[0052] Set 50, 100, 200, 400μM four concentrations of dopamine aqueous solution, spray 30 tablets of each of the four concentrations of dopamine aqueous solution on the harvested 'Dangshan' pear leaves with clear water treatment as a control, and inoculate the ringworm after 24 hours of treatment. The diameter of the lesion was counted every two days.

[0053] Indoor spraying of different concentrations of dopamine test results are as follows: figure 1 As shown, 50, 100, 200, 400μM four concentrations of dopamine treatment can inhibit the growth of pear ring disease, and the diameter of the lesion decreased by 41.6%, 71.6%, 54%, 62.3%, respectively. Among them, 100 μM dopamine has the best treatment effect, so subsequent experiments all use this concentration for treatment.

Embodiment 2

[0055] Three plants of 'Cuiguan', 'Sucui No.1' and 'Yuluxiang' with the same tree vigor, tree age and load capacity were selected for the test. Spray 100 μM dopamine solution and add 1% Tween80 every 15-20 days during the period of fruit expansion. The spraying time of 'Su Cui No. 1' is (May 1, May 21, June 11, July 1, 2020) and the spraying time of 'Cuiguan' is ( May 1st, May 21st, June 11th, July 1st, July 21st, 2020) were sprayed 5 times in total, and the spraying time of 'Yuluxiang' was (May 1st, 2020, May 21st, June 11th, July 1st, July 21st, August 10th, September 1st, September 20th) sprayed 8 times in total. The spraying time is 8-9 o'clock in the morning when the weather is fine, or 4-5 o'clock in the afternoon, and spraying with clear water is used as a control test.

[0056] Select pear fruits with the same maturity and size without mechanical damage and pests, wash the surface with sterile water, pierce the middle of the fruit with a sterilized needle, pierce a h...

Embodiment 3

[0059] Three 'Dangshan' pears with the same tree vigor, tree age and load were selected for the experiment.

[0060] The pear leaves were punctured at the upper and lower positions on the left and right sides, inoculated with the pathogenic bacteria of ring print, cultured at 25°C, and the diameter of the lesion was measured at 0, 2, 4, 6, and 8 days respectively. Dangshan’ pear leaves were treated with 100 μM dopamine and inoculated with the pathogenic bacteria Figure 8 The lesion diameters showed that the average lesion diameters on days 2, 4, 6, and 8 were significantly lower than those treated with CK, and decreased by 62.2%, 37.3%, 29.1%, and 26.1%, respectively.

[0061] The activities of phenylalanine ammonia lyase (PAL), superoxide dismutase (SOD), peroxidase (CAT), peroxidase (POD), Hydrogen peroxide (H 2 o 2 ) content and superoxide anion (OFR) content. Enzyme activity, hydrogen peroxide and superoxide anion content test results reference Figure 9-13 , as show...

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Abstract

The invention belongs to the technical field of fruit and vegetable disease prevention and control, and particularly relates to a method for preventing and controlling pear ring rot by using dopamine, which comprises the following steps: spraying a pear tree body by using a dopamine aqueous solution with the concentration of 50-400 mu M every 15-20 days in a pear fruit swelling period. According to the method, the activity of phenylalanine ammonialyase, superoxide dismutase, peroxidase and peroxidase is improved by spraying dopamine, the ability of removing hydrogen peroxide and superoxide anions in pear trees infected by pathogenic bacteria is improved, the autophagy activity of cells is improved, and the development speed of the diameter of disease spots is delayed; therefore, the occurrence of ring spot is reduced.

Description

technical field [0001] The invention specifically relates to a method for preventing and controlling pear ring spot by using dopamine, and belongs to the technical field of fruit tree disease prevention and control. Background technique [0002] Pear ring disease, also known as rough skin disease and tumor skin disease, is an important disease in pear tree production. China is the largest pear producer in the world, and its harvested area, output, export volume and number of varieties rank among the top in the world. my country's pear fruit planting range is wide, and all the provinces (autonomous regions, municipalities directly under the Central Government) have pear trees cultivated in the whole country except Hainan Province, Hong Kong and Macao. The ringworm fungus can cause diseases on pear fruits, branches and leaves. Damage to branches can cause premature senescence of branches, damage to leaves can cause premature leaf fall, and damage to fruits can cause a large nu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G13/00A01G7/06
Inventor 张绍铃张镇武谢智华王云孙逊毛舒悦
Owner NANJING AGRICULTURAL UNIVERSITY
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