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A combined preparation, system and application thereof for thrips control

A preparation and technology for thrips, which are applied in the field of combined preparations for thrips prevention and control, can solve problems such as the adverse effect of fungal germination, the fatigue effect of mutagen, and increase the cost of prevention and control, so as to avoid frequent use of fungi, avoid inhibitory effects, and avoid unfavorable effects. effect of influence

Active Publication Date: 2022-05-20
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The use of ultraviolet mutagenesis may cause mutagen fatigue effects, and the performance is unstable. In actual production, it is necessary to use several mutagens to compound and cross-use methods for strain mutagenesis, which increases the cost of prevention and control. During the process, we also found that some food attractants can have adverse effects on the germination of fungi

Method used

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  • A combined preparation, system and application thereof for thrips control
  • A combined preparation, system and application thereof for thrips control
  • A combined preparation, system and application thereof for thrips control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1: Preparation of Beauveria bassiana conidia and food attractant

[0082] 1. Acquisition of conidia of Beauveria bassiana

[0083] The conidia of Beauveria bassiana are taken from Baoji Duobao, a commercial product ® (15 billion spores / g Beauveria bassiana wettable powder, Beijing Zhongbao Green Agricultural Technology Group, pesticide registration number: PD20183086). Inoculate the spores into Goettel sporulation medium (2% corn flour, 1% wheat bran, 0.5% peptone, 0.3% KH 2 PO 4 , 0.1% MgSO 4 •7H 2 O, 0.1% NH 4 NO 3 , 2% agar, 1L distilled water), cultured continuously at 26±1°C for 14 days, and collected the cultured spore powder. Using sterilized 0.05% Tween-80 solution as a solvent, conidia powder was prepared into a suspension, and then inoculated into a 100 mL Erlenmeyer flask containing 50 mL germination solution (4% glucose, 1% yeast extract), to a final concentration of 10 7 Spores / mL (determined by hemocytometer), 26°C, 180rpm, spin culture...

Embodiment 2

[0091] Embodiment 2: Indoor experiments detect the influence of food attractants on the germination of conidia of Beauveria bassiana

[0092] Weigh 0.01g of Beauveria bassiana conidia powder, 15 billion spores / g, spread evenly on a cover glass (2.5×2.5cm), transfer to a petri dish containing water agar (2% w / v) ( 12cm) on. Put 0.3ml of the food attractant in scheme 2 into a non-woven bag (3×4cm, Weifang Jinnong) and set aside.

[0093] Indoor open space (see figure 1 Left image): Setting up R 0 , R 10 , R 20 (The distance between the food attractant and the conidia is 0, 10, 20cm) three groups respectively represent the vertical distance between the food attractant and the petri dish (with conidia) is 0, 10, 20cm, and the food attractant The non-woven bag was hung directly above the petri dish, and no food attractant was hung as a control. Each treatment was repeated 3 times.

[0094] confined space (see figure 1 Right picture): The petri dish (with conidia) is place...

Embodiment 3

[0097] Example 3: Field experiment to evaluate the influence of the distance between the food attractant and the device on the germination rate of Beauveria bassiana, the amount of thrips attracted and the amount of bacteria obtained by thrips on the surrounding plants

[0098] The field experiment was carried out in a greenhouse in Shiziying Village, Changping District, Beijing. eggplant Solanum melongena Planted in a greenhouse, no chemical pesticides were used during the experiment, and other managements were normal. Field experiments were conducted to evaluate the effects of the distance between the food attractant (Scheme 2) and the device on the germination rate of Beauveria bassiana, the amount of thrips attracted, and the amount of bacteria collected by thrips on surrounding plants.

[0099] The combined application device of food attractant and Beauveria bassiana used in this study is as follows: figure 2 shown. The carton (container) (10×10×15cm) has 1 large acc...

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Abstract

The invention discloses a combined preparation, system and application of thrips. The combined preparation includes Beauveria bassiana and a food attractant, and the food attractant and Beauveria bassiana are used in combination, mixed or not. , on the basis of increasing the amount of attraction to thrips, at the same time, it does not affect the germination of conidia of Beauveria bassiana, thereby improving the transmission efficiency of thrips to Beauveria bassiana, and achieving a continuous biological control effect.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control, and in particular relates to a combined preparation, system and application for thrips control. Background technique [0002] Thrips belong to the insects of Thysanoptera (Thysanoptera), and are a dangerous worldwide pest. Common thrips include melon thrips, onion thrips, rice thrips, western flower thrips and yellow thrips, etc. The insect is widely distributed all over the world, and has complex feeding habits, mainly herbivorous, fungiphagous and predatory, of which herbivores account for more than half, and it is one of the important economic pests. Thrips pose serious damage to many crops, horticultural crops and commercial crops by feeding, laying eggs and spreading viruses. Due to their small size and hidden living habits, thrips are difficult to control. At present, chemical agents are commonly used for thrips control, but chemical agents are not friendly to the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/30A01N31/16A01N49/00A01N43/40A01N35/04A01N31/04A01N65/40A01N25/18A01P19/00A01P7/04A01M1/02A01G13/00
CPCA01N63/30A01N31/16A01N49/00A01N43/40A01N35/04A01N31/04A01N65/40A01N25/006A01N25/18A01N65/00A01M1/02A01G13/00A01M2200/012Y02A50/30
Inventor 王海鸿刘胜雷仲仁王帅宇王登杰
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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