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Method for preparing fucoidan by biological coupling method

A technology of fucoidan and coupling method, applied in the field of polysaccharide extraction, can solve the problems of destruction of active components, high production cost, low extraction efficiency of fucoidan, etc., achieves rapid reaction, large processing capacity, and is conducive to large-scale production Effect

Inactive Publication Date: 2022-03-18
山东筱萤生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems of low extraction efficiency of existing fucoidan, high production cost, and large destruction of active ingredients

Method used

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  • Method for preparing fucoidan by biological coupling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Raw material pretreatment: Wash and dry the kelp with tap water, cut the dried kelp into small pieces with a knife, and crush it to 80 meshes;

[0025] (2) Biological enzymatic hydrolysis: Add 1:30 water to the kelp powder obtained in step (1), adjust the pH to 4.8 with hydrochloric acid, add 1% acid cellulase, 0.5% acid pectinase, and stir at 50°C 2h; adjust the pH of the enzymolysis solution to 8.0, add 0.2% alkaline pectinase, and carry out the enzymolysis reaction at 60°C for another 1.5 hours;

[0026] (3) Biological fermentation: Sterilize the enzymatic hydrolyzate obtained in step (2), add Aspergillus niger and Stenotrophomonas maltophilia for mixed fermentation, the inoculum ratio is 1:1, and the total inoculum amount: 10%. The fermentation condition is 30°C, 200r / min fermentation for 72h;

[0027] (4) Calcium precipitation: After the fermentation, the fermentation product was centrifuged, and 30% calcium chloride was added to filter to remove the precipita...

Embodiment 2

[0029] (1) Raw material pretreatment: Wash and dry the kelp with tap water, cut the dried kelp into small pieces with a knife, and crush it to 80 meshes;

[0030] (2) Biological enzymatic hydrolysis: Add 1:40 water to the kelp powder obtained in step (1), adjust the pH to 5.0 with hydrochloric acid, add 5% acid cellulase, 1% acid pectinase), at 50°C Stir for 2 hours; adjust the pH of the enzymolysis solution to 9.0, add 0.2% alkaline pectinase, and carry out the enzymolysis reaction at 60°C for 1.5 hours;

[0031] (3) Biological fermentation: Sterilize the enzymatic hydrolyzate obtained in step (2), insert yeast and lactic acid bacteria for mixed fermentation, the inoculum ratio is 1:2, and the total inoculation amount: 9%. The fermentation condition is 30°C, 200r / min fermentation for 48h;

[0032] (4) Calcium precipitation: After the fermentation is over, centrifuge the fermentation product, add 35% calcium chloride to filter to remove the precipitate, and collect the supern...

Embodiment 3

[0035] (1) Raw material pretreatment: Wash and dry the kelp with tap water, cut the dried kelp into small pieces with a knife, and crush it to 100 mesh;

[0036] (2) Biological enzymatic hydrolysis: Add 1:50 water to the kelp powder obtained in step (1), adjust the pH to 5.0 with hydrochloric acid, add 3% acid cellulase and 1% acid pectinase, and stir at 50°C 2h; adjust the pH of the enzymolysis solution to 8.0, add 0.5% alkaline pectinase, and carry out the enzymolysis reaction at 60°C for another 1.5 hours;

[0037] (3) Biological fermentation: Sterilize the enzymatic hydrolyzate obtained in step (2), insert Bacillus subtilis and Stenotrophomonas maltophilia for mixed fermentation, the inoculum ratio is 1:1, the total inoculation amount: 5% . The fermentation condition is 30°C, 200r / min fermentation for 48h;

[0038] (4) Calcium precipitation: After the fermentation, the fermentation product was centrifuged, and 40% calcium chloride was added to filter to remove the precip...

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Abstract

The invention belongs to the technical field of polysaccharide extraction, and particularly relates to an extraction method and application of fucoidan. The method comprises the following steps: cleaning kelp, drying, crushing, sieving, and adding composite biological enzyme for reaction to obtain enzymatic hydrolysate; inoculating probiotics into the enzymatic hydrolysate to carry out mixed fermentation, centrifuging the fermentation liquor, collecting the supernatant, and preparing fucoidan through calcium precipitation impurity removal and gradient alcohol precipitation. According to the fucoidan extraction method provided by the invention, the coupling effect of biological enzymolysis and probiotic fermentation is adopted, cell walls are cracked through the biological enzymolysis method to release internal solubles, macromolecules are degraded into micromolecules through probiotic fermentation, the extraction condition is mild, the energy consumption is low, the fucoidan extraction method is green and environment-friendly, and the efficacy performance and the product value of seaweed are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and in particular relates to a method for extracting fucoidan and its application. Background technique [0002] Kelp is a cold-water macroeconomic algae, and it is one of the main algae products produced in my country. Kelp is not only a nutrient-rich marine vegetable, but also rich in nutrients and a variety of minerals, and has extremely high economic value in industry and food processing. At the same time, it is rich in economic components such as fucoidan, alginate, mannitol, and iodine, and is also an important raw material for industries such as medicine and health care, seaweed chemical industry, and agricultural fertilizers. Over the past 50 years, more than 3,000 products derived from algae have been discovered, attracting great attention and considered as the most promising anticancer active substances. With the continuous development of marine natural products, fuc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/04C08B37/00C12R1/685C12R1/125C12R1/01C12R1/645C12R1/225
CPCC12P39/00C12P19/04C08B37/0063
Inventor 岳秋林赵林赵晨李宝君苏乐其他发明人请求不公开姓名
Owner 山东筱萤生物科技有限公司
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