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Artemisia lindleyana alcohol extract as well as extraction method and application thereof

An extraction method, a technology of artemisinin, applied in the field of ethanol extract of Artemisia annua Central Asia and its extraction

Active Publication Date: 2022-03-18
XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report about the use of Artemisia annua extracts in the preparation of immune-enhancing drugs

Method used

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  • Artemisia lindleyana alcohol extract as well as extraction method and application thereof
  • Artemisia lindleyana alcohol extract as well as extraction method and application thereof
  • Artemisia lindleyana alcohol extract as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Crush the above-ground part of Artemisia chinensis, pass through a 40-mesh sieve, collect the powder under the sieve, add it to 85% ethanol aqueous solution, and the ratio of solid to liquid is 1:10 (g:mL). After it was fully mixed, it was placed in a water bath at 60°C, and extracted at a constant temperature for 3 times, 2 hours each time. After the extraction, the solid and liquid were separated, and the supernatants were combined, concentrated by rotary evaporation until there was no ethanol smell, and freeze-dried to obtain 85% ethanol extract of Artemisia chinensis. Dissolve the 85% alcoholic extract of Artemisia subtilis in distilled water, adjust it to a concentration of 60mg / mL alcoholic extract solution, pass through the AB-8 macroporous resin column, and successively dissolve with water, 30%, 50%, 70%, and 85% ethanol Gradient elution of aqueous solution, absolute ethanol, etc., each gradient eluted with 4 column volumes, among which the components eluted wit...

Embodiment 2

[0047] Crush the above-ground part of Artemisia chinensis, pass through a 50-mesh sieve, collect the powder under the sieve, add it to 80% ethanol aqueous solution, and the ratio of solid to liquid is 1:12 (g:mL). After it was fully mixed, it was placed in a 65°C water bath, and extracted twice at a constant temperature, each time for 3 hours. After the extraction, the solid and liquid were separated, and the supernatants were combined, concentrated by rotary evaporation until there was no ethanol smell, and freeze-dried to obtain 80% ethanol extract of Artemisia chinensis. Dissolve the 80% alcoholic extract of Artemisia chinensis with distilled water, adjust it to a concentration of 60 mg / mL alcoholic extract solution, pass through the AB-8 macroporous resin column, and successively use water, the volume concentration is 30%, 50%, 70%, 85% ethanol aqueous solution and absolute ethanol gradient elution, each gradient elution has 3 column volumes, after screening, the optimal c...

Embodiment 3

[0050] Crush the above-ground part of Artemisia annua, pass it through a 30-mesh sieve, collect the powder under the sieve, add it to 90% ethanol aqueous solution, and the ratio of solid to liquid is 1:8 (g:mL). After it was fully mixed, it was placed in a water bath at 55°C, and extracted at a constant temperature for 3 times, each time for 3 hours. After the extraction, the solid and liquid were separated, and the supernatants were combined, concentrated by rotary evaporation until there was no ethanol smell, and freeze-dried to obtain 90% ethanol extract of Artemisia chinensis. Dissolve the 90% alcoholic extract of Artemisia subtilis in distilled water, adjust it to a concentration of 60mg / mL alcoholic extract solution, pass through the AB-8 macroporous resin column, and successively add water, 30%, 50%, 70%, and 85% ethanol aqueous solution , anhydrous ethanol and other gradient elutions, each gradient elution has 4 column volumes, and the components eluted with 30% ethano...

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PUM

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Abstract

The invention provides an artemisia rupestris alcohol extract as well as an extraction method and application thereof, belongs to the technical field of biological medicines, and particularly relates to a component collected after 80-90% of an ethanol extract of the artemisia rupestris is eluted by macroporous adsorption resin and 30% of ethanol. Experiments show that the AAEM provided by the invention promotes the expression of DC surface costimulatory molecules CD40 and CD86, and can improve the level of DC secreting IL-6 and TNF-alpha. The AAEM shows the ability of promoting DC maturation in an in-vitro experiment, which shows that the AAEM has an immunological enhancement effect, can provide a candidate material for screening a safe and efficient immunomodulator, and can be used as a candidate drug for tumor and inflammatory infection related diseases.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an ethanol extract of Artemisia chinensis and its extraction method and application. Background technique [0002] Dendritic cell (DC) is a powerful professional antigen-presenting cell, which connects the innate immune system and the adaptive immune system, balances the adaptive response between self and non-self, has antigen presentation and induces T cells React function. With the deepening of people's research, the immune function regulation of dendritic cells can be used to treat diseases such as autoimmune diseases and tumors. In recent years, DC can induce anti-tumor immunity, and DC vaccines enhance the anti-tumor activity of the body. In modern medical technology, surgery, chemotherapy and radiotherapy are the conventional methods for treating cancer, but none of them can solve the problems of the treatment effect of advanced cancer and the great impact on the pati...

Claims

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Application Information

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IPC IPC(8): A61K36/282A61P29/00A61P35/00A61P37/04
CPCA61K36/282A61P37/04A61P35/00A61P29/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2236/55Y02A50/30
Inventor 李金耀马荣斌夏丽洁马梓航
Owner XINJIANG QIANJIN RONGYAO INVESTMENT CO LTD
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