Preparation method of DNA (Deoxyribose Nucleic Acid) virus vaccine

Abstract

The invention relates to a preparation method of a DNA (Deoxyribose Nucleic Acid) virus vaccine, which comprises the following steps of: sequencing genetic materials of a DNA virus, and determining a specific DNA sequence corresponding to a specific protein of the DNA virus; selecting engineering bacteria; intercepting a specific DNA sequence from a genetic material of the DNA virus, and introducing the specific DNA sequence into the engineering bacteria; carrying out fermentation cultivation on the engineering bacteria into which the specific DNA sequence of the virus is introduced by adopting specific fermentation equipment, so that the quantity of the engineering bacteria is increased; crushing the engineering bacteria subjected to fermentation culture, so that the engineering bacteria are crushed; extracting specific protein of the DNA virus from the crushed engineering bacteria; specific protein of the DNA virus is extracted, and a corresponding DNA virus vaccine is prepared; specific fermentation equipment specifically comprises a support, a tank body, a first temperature control module, a second temperature control module, a PH value control module, a culture solution adding device, a pressure control module, an oxygen content control module, a buffer tank and a waste liquid collecting tank. The safety and the stability of the DNA virus vaccine are improved.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, and in particular relates to a preparation method of a DNA virus vaccine. Background technique [0002] A virus is a non-cellular form composed of a nucleic acid molecule and a protein, an organic species between living and non-living organisms that lives parasiticly. Viruses parasitize in the human body or animal body, making human body or animal disease-causing. Viruses are mainly formed by a capsid formed by genetic material and protein, and the protein capsid is wrapped on the outside of the genetic material; according to the difference in genetic material, viruses are divided into DNA viruses and RNA viruses. Currently, the most effective way to prevent viruses is to inject vaccines into humans or animals, so that the immune system in humans or animals can produce antibodies to form immunity to viruses. At present, the common vaccine is inactivated vaccine. Inactivated vaccine ...

AI Technical Summary

Problems solved by technology

Inactivated vaccines have certain risks. When the inactivated vaccine is not completely inactivated, the virus injected into the human body has certain toxicity, which directly makes the injected human or animal body sick

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