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Breeding method of pinctada martensii growth character improved variety based on whole genome selection

A technology of Pinctada martensii and the whole genome, applied in genomics, biochemical equipment and methods, proteomics, etc., can solve problems that have not been reported, reduce environmental impact, improve breeding yield and quality, shorten The effect of the breeding cycle

Pending Publication Date: 2022-03-11
GUANGDONG ZHANJIANG PROVINCIAL LAB OF SOUTHERN MARINE SCI & ENG +1
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Problems solved by technology

[0004] At present, for the selection of growth traits of Pinctada martensii, a growth trait QTL map has been initially constructed, and a number of genes and SNP sites associated with growth traits have been mined, but the method of using genome-wide marker information for selection has not yet been established. see the report

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  • Breeding method of pinctada martensii growth character improved variety based on whole genome selection
  • Breeding method of pinctada martensii growth character improved variety based on whole genome selection

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Embodiment 1

[0029] 1. Phenotype determination and reference population establishment of Pinctada martensii

[0030] The new species of Pinctada martensii "Haixuan No. 1" and the golden-yellow shell-color breeding population were selected as research materials, and the growth traits including shell length, shell width, shell height, total weight, and shell weight were measured (Table 1) .

[0031] Table 1 Statistical analysis of phenotypic traits

[0032] character Mean ± standard deviation (mm) shell length 65.71±6.95 shell width 24.36±1.87 shell height 67.32±6.2 gross weight 44.52±8.91 Shell weight 20.12±3.85

[0033] 2. Reference group whole genome resequencing, genotype collection and analysis processing

[0034] Genomic DNA extraction and library sequencing were performed on the reference population.

[0035] Genomic DNA extraction: use TIANGEN's marine animal tissue genomic DNA extraction kit to extract genomic DNA, the specific steps ...

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Abstract

The invention discloses a breeding method of a pinctada martensii growth trait improved variety based on whole genome selection. The breeding method comprises the following steps: selecting a reference group by measuring phenotypes; obtaining SNP locus information in a whole genome range by using a sequencing technology; three methods of rrBLUP, BayesA and BayesB are used for estimating the effect value of the SNP, and the accuracy is analyzed; and estimating the GEBV of each individual in the reference group. According to the method, the pinctada martensii can be selected in the early stage, the breeding period is shortened, the investment is reduced, the character selection accuracy can be improved, and factors influencing the environment are reduced. The GEBV obtained by using the method can be used as a standard for breeding improved varieties with growth traits, an effective molecular breeding method is provided for breeding excellent varieties of Pinctada martensii with excellent growth traits, the growth traits, the breeding yield and the breeding quality of the Pinctada martensii are improved, and green and healthy development of the breeding industry of the Pinctada martensii is promoted.

Description

technical field [0001] The invention belongs to the technical field of aquatic genetics and breeding, and in particular relates to a method for breeding improved varieties of Pinctada martensii based on whole genome selection for growth traits. Background technique [0002] Pinctada fucata martensii is the main mollusc in my country to cultivate seawater nucleated pearls. At a stage in which the output of seawater pearls in my country continues to decline, the cultivation of fine varieties is one of the effective ways to deal with industrial problems. The combination of traditional breeding methods and genotyping technology can improve the accuracy and precision of trait selection, and more importantly, greatly shorten the breeding time, which has become the mainstream direction of current breeding. Cultivating new varieties suitable for local sea area breeding and improving the growth and pearl breeding traits of the cultured population are the primary tasks to solve the i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B30/10G16B20/20G16B40/00C12Q1/6888C12Q1/6869
CPCG16B30/10G16B20/20G16B40/00C12Q1/6888C12Q1/6869C12Q2600/124C12Q2600/156C12Q2525/191
Inventor 郝瑞娟邓岳文郑哲王庆恒杨创业
Owner GUANGDONG ZHANJIANG PROVINCIAL LAB OF SOUTHERN MARINE SCI & ENG
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