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Method for evaluating quality of pinellia ternate and Pinellia yaoluopingensis based on organic acid

A technology of organic acid and pinellia, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve the problem of inability to analyze the types of organic acid components

Pending Publication Date: 2022-02-18
HEFEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The 2015 edition of "Chinese Pharmacopoeia" records that the determination of the organic acid content of Pinellia adopts the potentiometric titration method, which is calculated based on the content of succinic acid for quality control; but this method can only roughly reflect the content of the organic acid components of Pinellia, and cannot analyze the organic Types of Acid Components
At present, there are few reports on the organic acid components of Yaoluoping Pinellia

Method used

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  • Method for evaluating quality of pinellia ternate and Pinellia yaoluopingensis based on organic acid
  • Method for evaluating quality of pinellia ternate and Pinellia yaoluopingensis based on organic acid
  • Method for evaluating quality of pinellia ternate and Pinellia yaoluopingensis based on organic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Extraction and Detection of Organic Acid Components of Pinellia Pinellia and Yaoluoping Pinellia

[0032] 1. Instruments and reagents

[0033] (1) Instruments: ultra-high performance liquid chromatography UPLC (SHIMADZU Nexera X2) and tandem mass spectrometer MS / MS (Applied Biosystems 4500QTRAP), high-speed universal pulverizer.

[0034] (2) Reagents: The tubers of Pinellia and Yaoluoping Pinellia were provided by the School of Life Sciences, Hefei Normal University. Ethanol, acetonitrile, formic acid and ethyl acetate were of chromatographic grade, and other reagents were of analytical grade.

[0035] 2. Material pretreatment

[0036] Freshly collected Pinellia tubers and Pinellia tubers of Yaoluoping were washed with clean water, sliced, dried in an oven at 105°C for 15 minutes, and then dried at 60°C until constant weight (48h). Subsequently, after grinding into powder with a high-speed universal pulverizer, pass through a 60-mesh sieve.

[0037] 3. Extra...

Embodiment 2

[0057] Example 2: Screening of Different Organic Acid Components of Pinellia and Yaoluoping Pinellia

[0058] 1. Quantitative analysis of organic acid components

[0059] Using the software Analyst 1.6.3 to process the mass spectrometry data, obtain the total ion chromatogram (TIC) of the metabolites of Pinellia and Yaoluoping Pinellia. The characteristic ions of each substance were screened out by a triple quadrupole, and the chromatographic peaks were integrated and corrected with MultiaQuant software to complete the quantitative analysis of organic acid components.

[0060] 2. Screening of differential organic acid components

[0061] Comparing the chromatographic peak integration (peak area) of different organic acid components of Pinellia and Pinellia yaoluoping, the components with a difference of more than 2 times or less than 0.5 times are the screened differential organic acid components.

[0062] Depend on figure 1 and figure 2 It can be seen that the peak shape...

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Abstract

The invention discloses a method for evaluating quality of pinellia ternate and Pinellia yaoluopingensis based on organic acid, and belongs to the field of chemical component analysis of traditional Chinese medicines. The method comprises the following steps: using 75% ethanol for heating reflux extraction of total organic acid components of pinellia ternate and Pinellia yaoluopingensis, using ethyl acetate for repeated extraction, removing impurities, and obtaining an organic acid extracting solution; detecting the extracting solution by using UPLC-MS / MS, qualitatively analyzing the organic acid components by using the standard substance database, totally identifying 109 organic acid components, performing quantitative detection by using a multi-reaction detection mode MRM of triple quadrupole mass spectrometry, and screening 91 organic acid components with significant content difference in combination with a difference multiple greater than 2 times or less than 0.5 times. The invention provides an excellent technical means for evaluating the quality of pinellia ternate and Pinellia yaoluopingensis by using organic acid, and has important application value in quality control and variety identification of pinellia medicines.

Description

technical field [0001] The invention belongs to the field of analysis of chemical components of traditional Chinese medicines, and more specifically relates to a method for evaluating the quality of pinellia and yaoluoping pinellia based on organic acids. Background technique [0002] Pinellia ternate is a perennial herbaceous plant of the genus Pinellia in the family Araceae, mainly distributed in Hubei, Henan, Anhui, Shandong, Sichuan and other places; it is used as medicine with tubers, and has the functions of antitussive, expectorant and antiemetic. , sedative, hypnotic, anti-tumor and other effects, it is a bulk medicinal material commonly used in our country. Yaoluoping Pinellia (Pinelliayaoluopingensis) is an endemic species in Yaoluoping National Nature Reserve in Anhui Province. It has similar efficacy with Pinellia, but its medicinal effect is faster than that of Pinellia, which may be related to its chemical composition. [0003] Studies have shown that organic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 欧祖兰焦春燕王占军朱小茜陈倩颖徐忠东
Owner HEFEI NORMAL UNIV
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