Steamed solomonseal rhizome polysaccharide and application thereof in regulating intestinal microorganisms
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A polysaccharide polysaccharide, steaming technology, applied in application, polysaccharide/gum-containing food ingredients, food science and other directions, can solve problems such as little attention
Active Publication Date: 2022-02-18
JIANGNAN UNIV +1
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Problems solved by technology
However, little attention has been paid to steamed Polygonatum, especially the efficacy of its polysaccharides
Method used
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Embodiment 1
[0064] A method of extracting steaming yellow stroke polysaccharide (HWP), including the steps of:
[0065] (1) Take the steamed yellow crush after nine steamed nine, and the 40 mesh sieve;
[0066] (2) Weigh 1 kg step (1) after sieving powder, add 10 L of the volume fraction of 80% ethanol solution, extracting 2 h at 60 ° C, repeatedly extracting twice, to remove lipid solubility; Residual ethanol;
[0067] (3) According to the material ratio 1: 12.5 (kg / L), 0.5 kg of the remaining material of step (2) was added, and 6.25 L of water was added, heated at 80 ° C for 2 hours, 2 hours each time; combined extract, decompression EtOAc (EtOAc) EtOAc.
[0068] (4) Wash the precipitate of step (3) with a volume fraction of 80% ethanol solution twice, resulting in crude polysaccharide;
[0069] (5) 500 ml of water, solubility polysaccharide is added to the crude polysaccharide of 5 g of step (4), resulting in a polysaccharide solution having a mass concentration of 1%, after which ammoni...
Embodiment 2
[0081] Example 2 HWP high dose group (HWPH)
[0082] A method of increasing the number of intestinal benefits and increasing short chain fatty acids and long chain fatty acid concentrations, including the following steps:
[0083] 400 mg of Example 1 was prepared by the steaming yellow and 22.5 ml of medium, 2.5 ml of IGF mixed, cultured in anaerobic environment at 37 ° C; 7 different fermentation time samples (0, 6, 12) 24, 36, 48 and 60 hours) and preserved at -80 ° C;
[0084] Wherein, the medium is prepared from: 1L aseptic phosphate buffer (pH = 6.8), protein 胨 2g, yeast extract 2g, sodium chloride 0.1 g, 0.04 g of hydrogen phosphate, potassium phosphate 0.04 g, seven water sulfate 0.01g, six hydrogen calcium calcium calcium calcium calcium calcium, sodium bicarbonate 2G, 80- Tween 2ml, high temperature sterilization, add: heme 0.02g, vitamin K1 10ml, pig bile salt 0.5g, half Caside hydrochloride salt 0.5 g;
[0085] Human feces bacterial liquid (IGF) Preparation: 10G feces f...
Embodiment 3
[0086] Example 3 HWP low dose group (HWPL)
[0087] Adjusting the evaporation of the evaporated yellow concentrate in Example 2 was 200 mg, which was consistent with Example 2.
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Abstract
The invention discloses steamed solomonseal rhizome polysaccharide and application thereof in regulating intestinal microorganisms, and belongs to the field of food. The method for extracting the steamed solomonseal rhizome polysaccharide comprises the following steps: (1) crushing and sieving steamed solomonseal rhizome which is steamed and dried for nine times; (2) extracting the crushed powder with an ethanol aqueous solution; then evaporating to remove residual ethanol in residues; (3) adding water into the residues according to a solid-liquid ratio of 1: 10-1: 15 (kg/L), extracting at 70-90 DEG C for 1.5-2.5 hours, filtering, concentrating the filtrate, and then adding absolute ethyl alcohol to precipitate polysaccharide; (4) washing the precipitate with an ethanol aqueous solution to obtain crude solomonseal rhizome polysaccharide; (5) adding water into the crude solomonseal rhizome polysaccharide for redissolving, then precipitating protein, separating a water phase, dialyzing, concentrating and drying to obtain the steamed solomonseal rhizome polysaccharide. According to the steamed solomonseal rhizome polysaccharide disclosed by the invention, the levels of SCFAs and LCFAs are increased, and the levels of beneficial bacteria such as bifidobacterium, bacteroides, Parabacteroides and the like are increased.
Description
Technical field [0001] The present invention relates to a steaming yellow color polysaccharide and its application in the adjustment of intestinal microorganisms belongs to the field of food. Background technique [0002] Short-chain fatty acids (SCFAS) and long chain fatty acids (LCFAS) have found a variety of useful effects. Such as improving insulin resistance, providing neuroprotective, relieves chronic inflammation, etc. The carbohydrate is obtained for biological transformation. The biological conversion process has intestinal flora participation, and the intestinal flora is more inclined to complex carbohydrates, such as polysaccharides, to facilitate the diversity of intestinal flora. However, the study of specific correlation changes in SCFAS, LCFAS and intestinal flora during polysaccharide conversion is not clear. [0003] Polygonatum Sibiricum serves as a representative dual-use medicinal material rich in polysaccharide, has been reported to have antioxidant, anti-inf...
Claims
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