Stimuli-responsive nano material and application thereof in preparation of in-situ tumor vaccine

Abstract

The invention relates to a stimuli-responsive nano material, which can be combined with an antigen generated by cryoablation to form a nano in-situ tumor vaccine, can realize the functions of lymph node targeting, in-vivo tumor fragment adsorption, targeted activation of antigen-presenting cells, pH-responsive drug controlled release, drug loading and the like, and provides a new method and a new technology for tumor immunotherapy. On the one hand, tumor fragments generated by cryoablation retain more antigen information, and an antigen presentation system can be activated more efficiently; and on the other hand, the stimuli-responsive nano material effectively adsorbs tumor fragments and can carry a pattern recognition receptor stimulant or drug, so that the ability of targeting and activating antigen-presenting cells is enhanced. According to the stimuli-responsive nano in-situ tumor vaccine prepared by the invention, tumor antigens generated by cryoablation are loaded, so that the defect that the cryoablation cannot kill far-end focuses and free tumor cells is overcome, and the stimuli-responsive nano in-situ tumor vaccine is expected to induce systemic immunotherapy while killing local focuses.

Description

technical field [0001] The disclosure belongs to the field of macromolecular materials, and in particular relates to a stimulus-responsive nanometer material and antigens produced by cryoablation to form nanometer in situ tumor vaccines, including related preparation methods and applications. Background technique [0002] In recent years, the development of tumor treatment methods represented by surgical resection, radiotherapy and chemotherapy has fallen into a bottleneck, while immunotherapy, as an emerging treatment mode, has shown higher application prospects in the treatment of various tumors. At present, tumor immunotherapy is mainly divided into two types: active immunotherapy and passive immunotherapy. Compared with passive immunization represented by CAR-T, antibody and cytokine therapy, active immunization represented by tumor vaccines is not only effective in many tumors. The curative effect is better, and it also has the advantages of long-term immunity and low a...

AI Technical Summary

Problems solved by technology

Although the tumor fragments produced in the tumor area after cryoablation cannot effectively activate the immune response due to the immunosuppressive environment in the tumor area and the insufficient activation of antigen-presenting cells, the antigenic information retained by these tumor fragments will not be changed because freezing will not change the biological characteristics. More complete, promising nano-tumor vaccines, providing more immunogenic fragments of antigens

Other tumor physical therapies such as radiotherapy, photothermal, radiofrequency ablation, and photodynamics can cause changes in the properties of tumor fragments. For example, photothermal and radiofrequency ablation can cause protein denaturation, and radiotherapy and photodynamic therapy can cause genetic material changes.

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