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On-site collection method of karst cave water environment DNA sample

An on-site collection and water environment technology, applied in the preparation of test samples, sampling devices, etc., can solve problems such as cross-contamination and DNA degradation, and achieve the effect of easy operation, simple operation, and avoiding inaccurate data

Pending Publication Date: 2022-02-15
柳州市渔业技术推广站
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide an on-site collection method for DNA samples of karst cave water environment. The existing DNA will degrade, cross-contamination and other problems, and the collection method is low in cost, easy to operate, and the accuracy of the collected samples is high

Method used

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  • On-site collection method of karst cave water environment DNA sample
  • On-site collection method of karst cave water environment DNA sample
  • On-site collection method of karst cave water environment DNA sample

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Embodiment 1

[0021] Embodiment 1: a kind of on-the-spot collection method of karst cave water environment DNA sample, comprises the following steps:

[0022] The first step is to cut a long enough traction rope - high-strength polyethylene fiber line, tie it on the top of the Baylor tube and the traction rod, and pull the Baylor tube to the cave water environment through the traction rod (bamboo pole, fishing rod, telescopic rod, etc.) Water is collected at the DNA testing point, and the collected water samples are put into disposable plastic basins for later use. The method is: if the water depth of the cave is less than or equal to 2 meters, take 1000mL of water samples 0.5m from the bottom of the water; if the water depth of the cave is more than 2 meters and less than 10 meters, take 1000mL of water samples at 0.5m below the water and 0.5m from the bottom and mix them Stand-by; if the water depth of the cave is greater than or equal to 10 meters, take 1000mL of water samples from 0.5m ...

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Abstract

The invention relates to an on-site collection method of a karst cave water environment DNA sample, which comprises the following steps: 1, collecting water from a karst cave water environment DNA detection point by using a Bailer tube, and putting the collected water sample into a disposable plastic basin for later use; 2, preliminarily filtering the DNA water sample in the karst cave environment collected in the step 1, and removing large-particle impurities, 3, taking a disposable syringe, sucking the preliminarily filtered water sample, connecting the water sample to a membrane-replaceable needle type filter loaded with a glass fiber membrane, manually extruding the syringe at a constant speed to enable the water sample to pass through the membrane-replaceable needle type filter, and then discharging the water sample, putting the replaced filter membrane into a centrifugal tube filled with anhydrous alcohol, and storing at low temperature. According to the method, the DNA sample in the karst cave water environment can be collected on site, the problems of DNA degradation, cross contamination and the like when a water sample is remotely transported to a laboratory for vacuum filtration are solved, and the collection method is low in cost, simple and convenient to operate and high in accuracy of the collected sample.

Description

technical field [0001] The invention relates to a method for collecting water environment DNA samples, in particular to an on-site collection method for water environment DNA samples in karst caves. Background technique [0002] The biodiversity of cave fish in Guangxi is very rich, and it is one of the hot spots for cave fish research in the world. The traditional method of research on fish diversity in karst caves mainly relies on collection methods such as net fishing, fishing, and electric shock. Due to the complexity and danger of underground karst caves, collection is extremely difficult, time-consuming and laborious. The progress of related research work is slow. The situation is grim. In recent years, environmental DNA technology has become an important tool for biodiversity research due to its high sensitivity, high efficiency, and low cost. The application of this technology can speed up the research process of cave fish diversity and meet the needs of sustainable...

Claims

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Application Information

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IPC IPC(8): G01N1/14G01N1/34
CPCG01N1/14G01N1/34
Inventor 罗福广文衍红王志强黄杰石国凡杨军
Owner 柳州市渔业技术推广站
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