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Intestinal cancer detection reagent and application thereof in intestinal cancer detection

A technology for detecting reagents and reagents, applied in the field of biomedicine, can solve the problems of low repeatability, complicated equipment operation, and small injection volume, etc., and achieve the effects of high specificity, accuracy, good stability and high repeatability

Pending Publication Date: 2022-02-11
JIANGSU XIANSIDA BIOTECH CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mass spectrometry instrument is precise, the operation of the equipment is complicated, and the mass spectrometer is expensive, so it is not suitable for popularization and use in clinical practice; (3) capillary electrophoresis (CE): capillary electrophoresis is low in cost, high in column efficiency, high in sensitivity, fast in speed, and easy to inject. The amount is small and the operation is simple, but the repeatability is not high and the stability is not as good as HPLC

Method used

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  • Intestinal cancer detection reagent and application thereof in intestinal cancer detection
  • Intestinal cancer detection reagent and application thereof in intestinal cancer detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Detection of intestinal cancer

[0053] Statistical analysis was carried out by measuring the G-Test specific fingerprint of serum glycoprotein oligosaccharide chains, and the materials and methods used:

[0054] 1. Test samples: Serum from colorectal cancer patients and normal controls.

[0055] 2. Experimental equipment: sugar group analyzer, PCR, centrifuge.

[0056] 3. Reagent preparation:

[0057] Reagent A: prepared by adding SDS with a mass concentration of 0.5 to 5% in ammonium bicarbonate solution with a concentration of 10 mM;

[0058] Reagent B: It is prepared by mixing 0.01~10U / 10μL glucosamidase and 0.01~10U / 10μL sialidase, and the pH value of the mixed solution is 4~9;

[0059] Reagent C: Prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO, the concentration is 0.01mM~1M;

[0060] Reagent D: stop solution.

[0061] The preparation method of the intestinal cancer detection reagent comprises the following steps:

[0062] Step ...

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Abstract

The invention provides an intestinal cancer detection reagent and a preparation method thereof, and the detection reagent is prepared by mixing the following reagents: a reagent A which is prepared by adding 0.5-5% by mass of SDS into an ammonium bicarbonate solution with a concentration of 10 mM; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase and has a pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide) and has a concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. A glycome atlas in serum is measured through a detection reagent, a peak value is quantified for statistical analysis, and the establishment method of the intestinal cancer serum glycome atlas model is provided for detecting intestinal cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for detecting intestinal cancer, in particular to a method for detecting intestinal cancer based on the specific fingerprint of serum glycoprotein oligosaccharide chain detection (G-Test). Background technique [0002] Intestinal cancer (Carcinoma ofrectum) is a common malignant tumor, including colon cancer and rectal cancer. The incidence of bowel cancer from high to low is rectum, sigmoid colon, cecum, ascending colon, descending colon and transverse colon. etc. are closely related. The age of onset of colorectal cancer tends to be older, and the ratio of male to female is 1.65:1. The occurrence of intestinal cancer is related to high-fat and low-fiber diet, chronic inflammation of the large intestine, colorectal adenoma, genetic factors and other factors such as: schistosomiasis, pelvic radiation, environmental factors (such as lack of molybdenum in the soil), smo...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
CPCC12Q1/34G01N2333/924G01N2333/978G01N2440/38G01N2800/06
Inventor 陈翠英王蕾谈宗男
Owner JIANGSU XIANSIDA BIOTECH CO LTD
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