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Hepatitis C liver cancer detection reagent and application thereof in hepatitis C liver cancer detection

A detection reagent, a technology for hepatitis C liver cancer, which is applied in the field of biomedicine, can solve the problems of complicated equipment operation, high sensitivity and small injection volume, etc., and achieves the effects of good stability, simple operation and high repeatability

Pending Publication Date: 2022-02-11
JIANGSU XIANSIDA BIOTECH CO LTD +1
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  • Claims
  • Application Information

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Problems solved by technology

However, the mass spectrometry instrument is precise, the operation of the equipment is complicated, and the mass spectrometer is expensive, so it is not suitable for popularization and use in clinical practice; (3) capillary electrophoresis (CE): capillary electrophoresis is low in cost, high in column efficiency, high in sensitivity, fast in speed, and easy to inject. The amount is small and the operation is simple, but the repeatability is not high and the stability is not as good as HPLC

Method used

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  • Hepatitis C liver cancer detection reagent and application thereof in hepatitis C liver cancer detection
  • Hepatitis C liver cancer detection reagent and application thereof in hepatitis C liver cancer detection

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Embodiment 1

[0050] Embodiment 1 detects hepatitis C liver cancer

[0051] Statistical analysis was carried out by measuring the G-Test specific fingerprint of serum glycoprotein oligosaccharide chains, and the materials and methods used:

[0052] 1. Test samples: Serum from patients with hepatitis C liver cancer caused by hepatitis C virus and normal controls.

[0053] 2. Experimental equipment: sugar group analyzer, PCR, centrifuge.

[0054] 3. Reagent preparation:

[0055] Reagent A: prepared by adding SDS with a mass concentration of 0.5 to 5% in ammonium bicarbonate solution with a concentration of 10 mM;

[0056] Reagent B: It is prepared by mixing 0.01~10U / 10μL glucosamidase and 0.01~10U / 10μL sialidase, and the pH value of the mixed solution is 4~9;

[0057] Reagent C: Prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO, the concentration is 0.01mM~1M;

[0058] Reagent D: stop solution.

[0059] 4. Glycosequencing detection steps:

[0060] Step 1 Preparation of...

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Abstract

The invention provides a hepatitis C liver cancer detection reagent and a preparation method thereof. The detection reagent is prepared by mixing the following reagents: a reagent A, which is prepared by adding 0.5-5% by mass of SDS into a 10 mM ammonium bicarbonate solution; a reagent B which is a mixed solution prepared by mixing 0.01-10 U / 10 [mu]L of glycosamine acylase and 0.01-10 U / 10 [mu]L of sialidase, and has the pH value of 4-9; a reagent C which is prepared by dissolving 8-aminopyrene-1,3,6-trisulfonic acid in DMSO (dimethylsulfoxide), and has the concentration of 0.01 mM to 1M; and a reagent D which is a stop solution. According to the invention, a glycome map in serum is determined by a detection reagent, and a peak value is quantified for statistical analysis, so that the invention provides a method for establishing a model of a hepatitis C liver cancer serum glycome map for detecting hepatitis C liver cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a detection method of hepatitis C liver cancer, in particular to a detection method of hepatitis C liver cancer based on the specific fingerprint of serum glycoprotein oligosaccharide chain detection (G-Test). Background technique [0002] Chronic Hepatitis C Virus (HCV) is one of the main causes of liver cirrhosis and liver cancer. Hepatitis C virus, referred to as hepatitis C for short, is a kind of viral hepatitis caused by hepatitis C virus (HCV) infection, which is mainly transmitted through blood transfusion, acupuncture, drug abuse and other ways. The pathological changes of hepatitis C are very similar to those of hepatitis B, with liver cell necrosis and lymphocyte infiltration being the main ones. Fibrous tissue proliferation in the portal area can occur in chronic hepatitis, leading to chronic inflammation, necrosis and fibrosis of the liver, and some patients may de...

Claims

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Application Information

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IPC IPC(8): C12Q1/34
CPCC12Q1/34G01N2333/924G01N2333/978G01N2440/38G01N2800/085
Inventor 陈翠英王蕾谈宗男苏瑞
Owner JIANGSU XIANSIDA BIOTECH CO LTD
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