Peucedanum praeruptorum dunn seedling breeding method

Abstract

The invention discloses a peucedanum praeruptorum dunn seedling breeding method. The method comprises the following steps: step 1, selection and treatment of explants: selecting fresh leaves without plant diseases and insect pests as the explants, sterilizing with a sterilizing agent, rinsing with sterile water, cutting off the edge parts of the leaves, and cutting the leaves into parts with size of 0.5-1.0 cm < 2 > and with leaf veins; 2, induction of callus: inoculating the explant treated in the step 1 into an induction culture medium, and carrying out dark culture for 50 days to obtain the callus, wherein the culture condition is that the temperature is (25 + / -2) DEG C, and dark culture is carried out; 3, callus proliferation culture: cutting the explant obtained in the step 2 into 0.5 cm < 2 >, inoculating the explant into a proliferation culture medium, and culturing for 30 days to obtain a large number of calluses; and 4, differentiation culture: cutting the callus obtained in the step 3 into 0.5 cm < 2 >, inoculating the callus into a differential culture medium, and culturing for 30 days to obtain a sterile test-tube plantlet, wherein the culture conditions are as follows: the temperature is (25 + / -2) DEG C, the illumination time is 12h / d, and the illumination intensity is 1000-1500LX.

Description

technical field [0001] The invention belongs to the technical field of Peucedanum tissue culture and breeding, and in particular relates to a breeding method of Peucedanum seedlings. Background technique [0002] Peucedanum is a perennial herb of the genus Peucedanum in the family Umbelliferae. The roots are used for medicinal purposes. The medicinal property is bitter, pungent, slightly cold, and returns to the lung meridian. Modern studies have shown that the root of Peucedanum contains a variety of coumarin components, of which keratin-type dihydropyranocoumarins are the main active components, which mainly have various pharmacological effects such as expectorant, antitussive, and asthma. Peucedanum has a medicinal history of thousands of years in my country. Scholars at home and abroad have done a lot of research on the chemical components and pharmacological effects of Peucedanum. Peucedanum is rich in chemical components, and the main components that have been found s...

AI Technical Summary

Problems solved by technology

At present, there are few research reports on the tissue culture of Peucedanum pectoralis