Method for rapidly evaluating germination resistance of quinoa ears

Abstract

The invention discloses a method for rapidly evaluating germination resistance of quinoa ears. The method comprises the following steps of preparing a grain germination culture medium, preparing culture mediums with different water activities, collecting mature quinoa ears, carrying out a grain germination test, carrying out grain germination determination and carrying out resistance evaluation. The pre-harvest germination resistance comprehensive index (RI) is used for weighting the germination rates of quinoa grains under different water activity conditions, so that the pre-harvest germination resistance of quinoa varieties can be reflected more objectively, and the method has the characteristics of being accurate in result, time-saving, labor-saving, good in repeatability, stable, reliable and the like.

Description

technical field [0001] The invention belongs to the technical field of plant protection, in particular to a method for rapidly evaluating quinoa panicle germination resistance. Background technique [0002] In recent years, quinoa has taken the world by storm. Based on its potential to combat climate change, maintain food security and excellent nutritional properties, the United Nations declared 2013 as the International Year of Quinoa. At present, there are about 90 countries that grow quinoa in the world, covering all continents of the world. The quinoa industry has a strong development momentum in my country and has become a rising star of small miscellaneous grain crops. The main planting provinces are Gansu, Inner Mongolia, Shanxi, Yunnan, Qinghai, Hebei and so on. It is estimated that by 2025, the domestic consumption demand for quinoa will exceed 100,000 tons, and quinoa is bound to become an important food crop. With the expansion of planting area, the ear germina...

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Problems solved by technology

[0003] At present, the methods for identifying the ear germination resistance of quinoa mainly include field delayed harvest method, whole ear germination method, and grain germination method. However, the problems existing in these methods mainly include: (1) Field delayed harvest method and whole ear germination method The wheat plants are tall and the ears are huge (1.5 m~2.0 m varieties can be found everywhere), and there are limitations in actual operation. It is difficult to accurately control the accuracy of the experiment and the data repeatability is low.

(2) The traditional whole ear germination method and grain germination method can only do one humidity gradient each time, and cannot simulate different humidity gradients; in addition, it is easy to rot and breed miscellaneous bacteria during the test period, which brings many uncertain factors to the whole experiment

(3) The traditional evaluation is to put quinoa as a whole into the artificial climate incubator, but the humidity error of most artificial climate incubators is relatively large, resulting in inaccurate identification of disease resistance

However, the traditional evaluation method is to evaluate the free water in the environment at a fixed value, which cannot accurately evaluate the sensitivity of quinoa grains to the free water available in the environment, the humidity is difficult to accurately control, and the reproducibility of the results is low

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