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A kind of Lactobacillus plantarum against Helicobacter pylori infection and its application

A technology against Helicobacter pylori and Lactobacillus plantarum, applied in the field of microbiology, can solve the problems of long course of disease, high recurrence rate, difficult to cure completely, achieve good gastric acid resistance, inhibit the growth of Helicobacter pylori, and improve the growth of Helicobacter pylori Effect

Active Publication Date: 2022-03-15
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gastric ulcer is a common clinical chronic digestive system disease with a long course of disease, high recurrence rate and difficult to cure completely

Method used

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  • A kind of Lactobacillus plantarum against Helicobacter pylori infection and its application
  • A kind of Lactobacillus plantarum against Helicobacter pylori infection and its application
  • A kind of Lactobacillus plantarum against Helicobacter pylori infection and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The present embodiment screens the bacterial strain that resists Helicobacter pylori infection from fermented kimchi, and the steps are as follows:

[0047] Select fermented kimchi samples, use 0.9% normal saline for 10-fold serial dilution, dilute 3 times, spread on MRS solid medium, and culture at 37°C for 48 h, pick typical bacteria and culture them on MRS solid medium The base surface was streaked and purified, and a single colony was picked and expanded in MRS liquid medium at 37°C, and then preserved in glycerol with a mass concentration of 30%.

[0048] The isolated single colony is screened according to its ability to inhibit the growth of Helicobacter pylori, the steps are as follows:

[0049] (1) Helicobacter pylori was taken out from -80°C, spread on Columbia blood agar medium containing 5% sheep blood (v / v) for activation culture, and cultured at 37°C for 48 h in a microaerobic environment;

[0050] (2) Inoculate the strain in MRS liquid medium, activate it...

Embodiment 2

[0055] In this embodiment, the morphological identification and 16S rRNA molecular biological identification of the strains screened in Example 1 are carried out, and the steps are as follows:

[0056] (1) Morphological identification:

[0057] The strains were inoculated in MRS solid medium, cultured at 37°C for 48 h, and observed under a microscope.

[0058] Observation shows that the colonies are milky white, semicircular and raised, with smooth and moist surfaces and neat edges.

[0059] (2) 16S rRNA molecular biology identification:

[0060]The strains stored at -80°C were taken out, inoculated in MRS liquid medium, and cultured at 37°C for 18 h. Pipette 1 mL of the bacterial liquid into a centrifuge tube, centrifuge at 8000 rpm for 10 min, remove the supernatant, and collect the bacterial cells.

[0061] The genome of the strain was extracted, and bacterial universal primers were added for PCR amplification, and the amplified products were sent to Shanghai Sangon Bioe...

Embodiment 3

[0066] The sequence obtained by sequencing was compared with the nucleic acid sequence in GeneBank, and the result showed that the strain was Lactobacillus plantarum.

[0067] Based on the results of 16S rRNA molecular biology identification and morphological identification of Example 2, it was confirmed that the bacterial strain belonged to Lactobacillus plantarum and was named as Lactobacillus plantarum Lactobacillus plantarum Lp05. The Lactobacillus plantarum was deposited on October 9, 2021 in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures. The address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, postcode 100101, and the preservation number is CGMCC No. . 23547.

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Abstract

The invention provides a plant lactobacillus resistant to Helicobacter pylori infection and its application, and the plant lactobacillus resistant to Helicobacter pylori infection is named as plantar lactobacillus Lactobacillus plantarum Lp05 was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.23547, and the preservation date was October 9, 2021. The invention also provides the cultivation method of the plant lactobacillus resistant to Helicobacter pylori infection and the product for inhibiting Helicobacter pylori infection. The plant lactobacillus resistant to Helicobacter pylori infection of the invention has good gastric acid resistance, inhibits the proliferation of Helicobacter pylori and reduces its adhesion to gastric epithelial cells, creating conditions for eradicating Helicobacter pylori. The product for inhibiting Helicobacter pylori infection does not cause adverse reactions and drug resistance of pathogenic bacteria, and has high application value.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a plant lactobacillus resistant to Helicobacter pylori infection and an application thereof. Background technique [0002] Helicobacter pylori ( Helicobacter pylori , Hp) was first discovered by Australian scholars Marshall and Warren in 1982. At present, Hp is classified as a class I carcinogen, and the infection rate of Hp in natural population exceeds 50%, and it shows an increasing trend year by year. [0003] Hp is a Gram-negative microaerophilic bacilli colonized in human gastric mucosa, and is the main pathogenic factor of chronic gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue lymphoma and gastric cancer. Studies have shown that there is a clear correlation between Hp and the occurrence and development of gastric ulcers, and more than 80% of gastric ulcers are caused by Hp infection. Gastric ulcer is a common clinical chronic digestive s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A23L33/135A23C9/123C12R1/25
CPCC12N1/20A61K35/747A61P31/04A23L33/135A23C9/1234A23V2002/00A23V2400/169A23V2200/30
Inventor 方曙光盖忠辉朱建国陈珂可
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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