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Nano-zinc oxide composite material based on bacillus thuringiensis as well as preparation method and application thereof

A technology of nano-zinc oxide and thuringiensis spores, applied in the field of biological materials, can solve the problems of imperfection, vacancies in Bt-ZnONPs yield, and differences in properties, and achieves low cost, no residual toxic reagents, and good dispersibility.

Active Publication Date: 2022-01-21
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is still not perfect in terms of preparation and practical application. There are still gaps in the technical optimization of the preparation process, the yield and property differences of Bt-ZnO NPs obtained under different conditions, so the preparation of Bt-ZnO NPs still needs to be done in the existing Continuing discussion and analysis based on research

Method used

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  • Nano-zinc oxide composite material based on bacillus thuringiensis as well as preparation method and application thereof
  • Nano-zinc oxide composite material based on bacillus thuringiensis as well as preparation method and application thereof
  • Nano-zinc oxide composite material based on bacillus thuringiensis as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Preparation of LB liquid medium: Add 1 g of yeast extract (purchased from Sigma, USA, CAS: 8013-01-2) and 2 g of tryptone to 200 mL of 0.9% sodium chloride solution, stir well until dissolved , autoclave sterilization, the sterilization conditions are: 0.15 Mpa, 125 ℃, 30 min, after cooling to room temperature;

[0032] (2) Bt culture: Inoculate the Bt strain (purchased from the China General Microorganism Culture Collection and Management Center, CGMCC 1.7882) into the culture dish containing (1) under aseptic conditions for strain culture. The culture conditions are: Temperature 35°C, 24h, to obtain Bt strains;

[0033] (3) Inoculate the Bt strain in step (2) into the slant culture medium under aseptic conditions, and expand the culture. The incubator conditions are: temperature 37°C, 150 rpm, 24 h, to obtain the Bt seed solution;

[0034] (4) Dilute the Bt seed solution in step (3) by 4 times with 0.9% sodium chloride solution, adjust the pH to 7, and continue c...

Embodiment 2

[0038] (1) Preparation of LB liquid medium: Add 1 g of yeast extract (purchased from Sigma, USA, CAS: 8013-01-2) and 2 g of tryptone to 200 mL of 0.9% sodium chloride solution, stir well until dissolved , autoclave sterilization, the sterilization conditions are: 0.15 Mpa, 125 ℃, 30 min, after cooling to room temperature;

[0039] (2) Bt culture: Inoculate the Bt strain (purchased from the China General Microorganism Culture Collection and Management Center, CGMCC 1.7882) into the culture dish containing (1) under aseptic conditions for strain culture. The culture conditions are: Temperature 35°C, 24 h, to obtain Bt strains;

[0040] (3) Inoculate the Bt strain in step (2) into the slant culture medium under aseptic conditions, and expand the culture. The incubator conditions are: temperature 37°C, 150 rpm, 24 h, to obtain the Bt seed solution;

[0041] (4) Dilute the Bt bacterial solution in step (3) by 4 times with 0.9% sodium chloride solution, adjust the pH to 7, and cont...

Embodiment 3

[0045] (1) Preparation of LB liquid medium: Add 1 g of yeast extract (purchased from Sigma, USA, CAS: 8013-01-2) and 2 g of tryptone to 200 mL of 0.9% sodium chloride solution, stir well until dissolved , autoclave sterilization, the sterilization conditions are: 0.15 Mpa, 125 ℃, 30 min, after cooling to room temperature;

[0046] (2) Bt culture: Inoculate the Bt strain (purchased from the China General Microorganism Culture Collection and Management Center, CGMCC 1.7882) into the culture dish containing (1) under aseptic conditions for strain culture. The culture conditions are: Temperature 35°C, 24 h, to obtain Bt strains;

[0047] (3) Inoculate the Bt strain in step (2) into the slant culture medium under aseptic conditions, and expand the culture. The incubator conditions are: temperature 37°C, 150 rpm, 24 h, to obtain the Bt seed solution;

[0048] (4) Dilute the Bt bacterial solution in step (3) by 4 times with 0.9% sodium chloride solution, adjust the pH to 7, and cont...

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Abstract

The invention discloses a nano-zinc oxide composite material based on bacillus thuringiensis as well as a preparation method and an application thereof. The preparation method comprises the following steps: (1) culturing bacillus thuringiensis (Bt for short); (2) preparing Bt diluent; and (3) synthesizing the nano-zinc oxide composite material by using Bt: adding zinc salt solution to the Bt diluent in the step (2) for conversion, and finally obtaining the nano-zinc oxide composite material Bt-ZnO NPs by adjusting influence factors such as bacterial liquid concentration, zinc acetate concentration, conversion temperature, time and reaction system pH. The preparation method has the characteristics of being simple in operation process, green, free of pollution, mild in reaction condition, and easy to operate. The prepared material has the advantages of being uniform in particle size, good in dispersity, and good in insect resistance.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a nano zinc oxide composite material based on Bacillus thuringiensis and its preparation method and application. Background technique [0002] Zinc oxide nanoparticles (ZnO NPs) have been widely used due to their antibacterial, antifungal, UV filtering properties, high catalytic activity and photochemical activity, becoming one of the hottest developing materials in recent years. Traditionally, the preparation of ZnO NPs materials is mostly physical or chemical methods, and the production process is mature and easy for industrial production, but there are problems such as complex process flow, high cost, environmental pollution, and high operational risk. In this context, biosynthetic nanomaterials are flourishing. Biosynthetic nanomaterials technology refers to the synthesis of NPs using natural materials such as microbial enzymes, polysaccharides, and biodegradable polymers...

Claims

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Application Information

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IPC IPC(8): C12P3/00A01N63/50A01N59/16A01N63/23A01P3/00A01P1/00A01P7/04C12R1/07
CPCC12P3/00A01N63/50A01N59/16A01N63/23Y02A50/30
Inventor 崔素芬吴云龙崔哲雨许玮黄念庭何鹏胡金龙唐歆玥
Owner JIANGSU UNIV OF SCI & TECH
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