Molecular marker closely linked with tomato neck and root rot resistant gene Frl, and application of molecular marker

Abstract

The invention discloses a molecular marker closely linked with a tomato neck and root rot resistant gene Frl, and application of the molecular marker. The invention provides application of a substance for detecting whether a 165bp fragment is contained in a positioning interval of an Frl gene in the genome of a tomato variety or not in identification or auxiliary identification of tomato neck and root rot resistance of tomatoes. The invention discovers that 165bp large fragment insertion related to the tomato neck and root rot resistance is in the presence in an intergenic region in the positioning interval (an interval of 4.2-5.1 Mb of a tomato No.9 chromosome) of the Frl gene in the genome of the tomato variety, a PCR-based molecular marker is designed according to the fragment, whether a tomato material to be detected contains the Frl gene or not and whether the tomato material is homozygous or not can be detected, a breeding period can be shortened, and the accuracy of breeding selection is improved.

Description

technical field [0001] The invention belongs to the field of plant molecular breeding, and in particular relates to a molecular marker closely linked with tomato neck rot and root rot resistance gene Frl and its application. Background technique [0002] Tomato neck rot and root rot is a very serious soil-borne disease, and its pathogen is Fusarium oxysporum tomato-specific. The typical symptoms of the disease are that the root of the plant rots downward from the soil line, the aboveground part wilts and loses water, the vascular bundle is browned, and the plant growth stagnates until death after infection with pathogenic bacteria. When the disease occurs, the tomato yield can be reduced by more than 65% or even no harvest, and the plant fatality rate can reach 100%. At present, there are two main methods for the prevention and treatment of tomato neck rot and root rot, one is physical and chemical control, and the other is to discover and use resistant resources to cultiva...

AI Technical Summary

Problems solved by technology

At present, the most widely used molecular marker in production is the co-dominant CAPS molecular marker C2-25 (Staniaszek, M., Szczechura, W., and Marczewski, W.2014. Identification of a new molecular marker C2-25 linked to the Fusariumoxysporum f.sp.Radicis-lycopersici resistance Frl gene in tomato.CzechJ.Genet.Plant Breed.50,285-287), but the detection process needs to use restriction endonuclease for enzyme digestion detection, the process is cumbersome, and some studies have shown that C2 The identification result of -25 is only 95.83%, which is not enough to accurately identify the resistance of the material (Liu Lei, Wang Hui, Li Wenli, Wang Fu 2018. Marker linked to tomato neck rot and root rot resistance gene Frl and its application. Jiangsu Agriculture Science 46,91-93)

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