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Method for improving yield of astaxanthin in wild phaffia rhodozyma strain through inorganic-microbial hybrid system

A yeast strain and microorganism technology, applied in the field of astaxanthin, can solve the problems of a large amount of input and low output of astaxanthin, and achieve the effect of increasing the output and having a good application prospect.

Active Publication Date: 2022-01-14
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The astaxanthin yield of wild Phaffia rhodozyme strains is very low. In actual production, methods such as mutagenesis breeding screening, protoplast fusion, and genetic engineering are used to obtain high-yielding Phaffia rhodozyme strains to increase astaxanthin production. In the early stage, a large amount of manpower and capital need to be invested in the screening of high-yield strains, and in the later stage, the production and culture conditions need to be continuously optimized

Method used

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  • Method for improving yield of astaxanthin in wild phaffia rhodozyma strain through inorganic-microbial hybrid system
  • Method for improving yield of astaxanthin in wild phaffia rhodozyma strain through inorganic-microbial hybrid system
  • Method for improving yield of astaxanthin in wild phaffia rhodozyma strain through inorganic-microbial hybrid system

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Method for enhancing a light-driven inorganic-microbial hybrid system to improve wild red jiff yeast shrimp production, including the following steps:

[0026] Shake bottle seed culture: Take a cyclopellar seed from the raw raw strain of the red husband yeast inoculated in seed medium, and the shake flask is cultured at 22 ± 5 ° C, and the rotational speed is 180-240 rpm. ;

[0027] Shake flask fermentation culture: According to a volume ratio of 10%, the seed culture solution is seeded in a fermentation medium containing a concentration of 0.2 g / L carbon nitride nanofer (eg Figure 4 As shown in the shake flask of the SEM map of the red jioff yeast and the carbon nitride nanofile self-assembly, the shake flask was cultured at 22 ± 5 ° C, and the rotational speed was 1808 hours under the speed of 180-240 rpm, of which fermentation culture The process is placed under completely dark conditions.

[0028] Among them, Hongfu Yeast Original Slapped strains were purchased in the...

Embodiment 2

[0044] Method for enhancing a light-driven inorganic-microbial hybrid system to improve wild red jiff yeast shrimp production, including the following steps:

[0045] Shake bottle seed culture: Take a cyclopellar seed from the raw raw strain of the red husband yeast inoculated in seed medium, and the shake flask is cultured at 22 ± 5 ° C, and the rotational speed is 180-240 rpm. ;

[0046]Shake flask fermentation culture: According to a volume ratio of 10%, the seed culture solution is seeded in a shake flask containing a concentration of 1 g / L carbon nitride nanofer fermentation medium, and the shake flask is at 22 ± 5 ° C, and the speed is 180- 240 rpm was cultured 48-108h, of which fermentation culture processes were placed under completely dark conditions.

[0047] Among them, Hongfu Yeast Original Slapped strains were purchased in the Stripe Square Savings Center of the Chinese Academy of Sciences, the strain number is 2.1557; the shake bottle seed medium includes 10-12 g / ...

Embodiment 3

[0050] Method for enhancing a light-driven inorganic-microbial hybrid system to improve wild red jiff yeast shrimp production, including the following steps:

[0051] Shake bottle seed culture: Take a cyclopellar seed from the raw raw strain of the red husband yeast inoculated in seed medium, and the shake flask is cultured at 22 ± 5 ° C, and the rotational speed is 180-240 rpm. ;

[0052] Shake flask fermentation culture: According to a volume ratio of 10%, the seed culture solution is inoculated into a shake flask containing a concentration of 5 g / L carbon nitride nanofer fermentation medium, and the shake flask is at 22 ± 5 ° C, and the speed is 180- 240 rpm was cultured 48-108h, of which fermentation culture processes were placed under completely dark conditions.

[0053] Among them, Hongfu Yeast Original Slapped strains were purchased in the Stripe Square Savings Center of the Chinese Academy of Sciences, the strain number is 2.1557; the shake bottle seed medium includes 10...

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Abstract

The invention discloses a method for improving the yield of astaxanthin in a wild phaffia rhodozyma strain by using an inorganic-microorganism hybrid system. The method comprises the following steps of: shake flask seed culture: taking an inoculating loop strain from an original slant strain slant of phaffia rhodozyma, inoculating a seed culture medium with the inoculating loop strain, and performing shake flask culture at 22 + / -5 DEG C and at the rotating speed of 180-240 rpm for 24-72 hours to obtain a seed culture solution; and shake flask fermentation culture: inoculating a carbon nitride nanosheet fermentation medium with the concentration of 0.2-6 g / L with the seed culture solution according to the volume ratio of 10%, and culturing a shake flask at the temperature of 22 + / -5 DEG C and the rotating speed of 180-240 rpm for 48-108 h, wherein the fermentation culture process is performed under the condition that the whole process is light or completely dark. According to the method, the yield of the astaxanthin of the phaffia rhodozyma can be remarkably increased by a method for assembling a hybrid system by using light-driven carbon nitride nanosheets and the phaffia rhodozyma.

Description

Technical field [0001] The present invention relates to the technical field of shrimpocyanins, and in particular, a method of improving the production of ateenin yield of wild red jiff yeast straw shrimp. Background technique [0002] Natural shrimp vitrin is called 3,3'-dihydroxy-4,4'-diketone -β, β'-carotene, is a terpene unsaturated compound containing a plurality of conjugated double bonds. It has strong quenching single-line oxygen and removal of free radicals, enhances body immunity and macrophage function, and has certain chemical prevention capabilities for cancer. Therefore, the shrimp vitros have great application value and broad development prospects in pharmaceutical, health products, cosmetics, food farming and other industries. [0003] Compared to artificial synthetic shrimp vitrin, natural shrimp vitrin is safe and stable, and the bioactive is high. And the microbial fermentation method has a low cost, which can have a low cost and can produce large-scale producti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12N1/16C12R1/645
CPCC12P23/00C12N1/16
Inventor 张伟娜霍峰蔚吴海波冯喜龙吉宁
Owner NANJING UNIV OF TECH
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