Preparation and application of high-throughput in-situ observable cell 3D culture plate

Abstract

The invention belongs to the field of biomedical materials, and particularly relates to preparation and application of a high-throughput in-situ observable cell 3D culture plate. The method comprises the following steps: 1, preparing alkylated SiO2 sol; 2, cleaning the cell culture plate; and 3, coating the alkylated SiO2 sol prepared in the step 1 on the surface of the cell culture plate in the step 2 to obtain the coated cell culture plate. The application is to prepare the single-layer nano-particle structure liquid marbles on the surface of a coated cell culture plate. The prepared super-hydrophobic SiO2 coating has the characteristic of weak binding force, and particles on the outermost layer are easy to transfer. The in-situ preparation of the liquid marbles in the porous plate can be realized by directly rolling the liquid drops of the cell culture fluid on the coating. The prepared liquid marbles can be directly used for 3D culture and high-throughput detection of cells. Compared with a powder source liquid marble, the liquid marble is more transparent, the internal situation can be observed more conveniently, and meanwhile waste and pollution caused by operations such as powder removal and marble transfer are avoided.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to the preparation and application of a high-throughput, in-situ observed cell 3D culture plate. Background technique [0002] Three-dimensional (3D) cell culture plays an important role in basic research and drug experimentation. A large number of studies have shown that three-dimensional cell culture can provide cell growth with living conditions closer to the living body, affect cell migration, aggregation, proliferation and differentiation, and maintain good biochemical and physiological responses of cells. [0003] Compared with the traditional two-dimensional cell culture, the cells in the three-dimensional environment divide in all directions, and the responses of the cells to endogenous and exogenous stimuli, such as temperature, pH, nutrient concentration, oxygen content, etc., are consistent with their in vivo . [0004] Traditional two-dimensional cell cu...

AI Technical Summary

Problems solved by technology

[0009] 1. The preparation of traditional liquid marbles uses powder as the particle source. After the liquid marbles are prepared, the excess powder must be removed, and the liquid marbles must be transferred to a platform such as a petri dish for subsequent operations. This is Qualcomm Massive cell culture and comparative analysis pose significant limitations;

[0010] 2. The surface particles of traditional powder liquid marbles will form clusters of tens of microns or more, resulting in poor transparency of liquid marbles, and it is often necessary to extract the internal substances for observation

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