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Application of peptide MOTS-c to preparation of medicine for treating Parkinson's disease

A technology of medicine, mots-c, applied in the field of medicine

Pending Publication Date: 2022-01-04
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the mechanism of action of peptide MOTS-c has not been fully elucidated, and there are no related reports that related peptide MOTS-c can be used to treat Parkinson's disease.

Method used

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  • Application of peptide MOTS-c to preparation of medicine for treating Parkinson's disease
  • Application of peptide MOTS-c to preparation of medicine for treating Parkinson's disease
  • Application of peptide MOTS-c to preparation of medicine for treating Parkinson's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 animal treatment

[0034] Peptide MOTS-c was synthesized by Wuhan Baiyixin Biotechnology Co., Ltd. with a purity of >95%. The amino acid sequence is shown in SEQ.ID.NO.1:

[0035] Met Arg Trp Gln Glu Met Gly Tyr Ile Phe Tyr Pro Arg Lys Leu Arg.

[0036] 44 male SD rats, weighing between 220 and 270 g, were kept in an animal room with an indoor temperature of 23±2°C, and the rats were provided with free drinking water and food during the period. Rats were randomly divided into 3 groups: control group, model group and treatment group. Model group (gradient dose subcutaneous injection): the injection dose in the first stage is 2 mg / kg, for a total of 3 days. In the second phase, the injection dose was 1 mg / kg, and the drug was administered for 7 days. In the third stage, the injection dose is 0.5mg / kg, administered for 20 days in total. A total of 30 days of administration were used to establish a rotenone-induced rat Parkinson's animal model. Treatment g...

Embodiment 2

[0037] Embodiment 2 implements each group of rat neuroethology experimental detection

[0038] 1) Open field test: The open field test is a method to evaluate the autonomous behavior, exploration behavior and tension of experimental animals in a new environment, so as to evaluate whether the animal's activity is normal and uniform. After adjusting the software and system parameters, put the rat in the open field reaction box and let it move freely for 10 minutes, and the system will record all the activity records of the rat. After the experiment is over, the software will record and analyze parameters such as the distance of movement, the trajectory of movement, and the number of standing times of hind limbs (before each experiment, the reaction box needs to be wiped with alcohol to eliminate the influence of odor on the animals).

[0039] 2) Rotating Rod Fatigue Test: By measuring the time the animal stays on the roller, it is used to evaluate the animal's balance, motor coo...

Embodiment 3

[0040] Embodiment 3 The content of rat striatum dopamine (high resolution liquid mass spectrometry method)

[0041] The striatum of the rat was weighed and the weight was recorded, 1 mL of methanol (containing 0.1% formic acid) was precisely added, vortexed for 1 min, and homogenized for 3 min. Centrifuge at 14,000 rpm for 10 min at high speed, and take the supernatant for analysis. Chromatography Waters T3 (150 x 2.1 mm, 3 μm). The flow rate is 0.3mL / min, the aqueous phase is 0.1% formic acid aqueous solution, the organic phase is 0.1% formic acid acetonitrile, the needle washing solution is methanol, and the temperature of the column oven is 35°C.

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Abstract

The invention relates to an application of a peptide MOTS-c to preparation of a medicine for treating Parkinson's disease. The peptide MOTS-c can reduce the level of oxidative stress injury to the midbrain of a rotenone-induced Parkinson rat animal model, protect rat midbrain nigra striatum dopaminergic neurons and reduce loss of dopaminergic neurons. The dopamine level of striatum is obviously improved, and the neurobehavioral symptoms of rats with Parkinson's disease are effectively improved. Therefore, the peptide MOTS-c has important application significance in preparation of the medicine for resisting the Parkinson's disease.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of a peptide MOTS-c in the preparation of medicines for treating Parkinson's disease. Background technique [0002] Parkinson's disease (PD) is the second most common neurodegenerative disease in the world, and its incidence increases with age, seriously affecting the health and quality of life of middle-aged and elderly people. The main pathological features of PD are the loss of dopamine neurons in the substantia nigra of the midbrain and the formation of Lewy bodies. With the reduction of dopamine in the striatum, resting tremor, muscle rigidity, slow movement, and abnormal posture and gait appear. Common clinical manifestations, the symptoms develop progressively, and in severe cases, patients cannot take care of themselves. At present, its etiology is not completely clear, and it is mainly considered to be the result of the interaction of multiple factors s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61K9/00A61P25/16
CPCA61K38/10A61K9/0019A61P25/16
Inventor 赛燕肖靖淞叶枫程晋张启夫单耀辉但国蓉张玺赵远鹏邹仲敏
Owner ARMY MEDICAL UNIV
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