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Lipid-coated albumin-bound chemotherapeutic drug nanoparticles as well as preparation method and application thereof

An albumin-bound, albumin nanotechnology, applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, can solve unseen problems, achieve reduced expression, high biocompatibility, The effect of reducing side effects

Pending Publication Date: 2022-01-04
JINSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no albumin-binding chemotherapeutic drug nanoparticle drug coated with lipids loaded with genes in the present application.

Method used

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  • Lipid-coated albumin-bound chemotherapeutic drug nanoparticles as well as preparation method and application thereof
  • Lipid-coated albumin-bound chemotherapeutic drug nanoparticles as well as preparation method and application thereof
  • Lipid-coated albumin-bound chemotherapeutic drug nanoparticles as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 50 mg of dioleoylphosphatidylethanolamine (DOPE) and 50 mg of (2,3-dioleoyl-propyl)-trimethylamine (DOTAP) were dissolved in 4 ml of chloroform, rotated at 40 degrees Celsius, and the solvent was evaporated to obtain lipid thin film. Add 5 ml of DEPC water for hydration, 37 watts of ultrasound for 5 minutes to obtain a lipid suspension, take 1 ml of the lipid suspension to freeze-dry and weigh, and determine the concentration of the lipid suspension to be 20 mg / ml.

Embodiment 2

[0059] Weigh 20 mg of human serum albumin nanoparticles (prepared by high-pressure homogenization using human serum albumin (purchased from sigma, product number A1653)), dissolve with 1 ml of normal saline, add 1 ml of the 20 mg prepared in Example 1 / ml of lipid suspension, 37 watts of ultrasound for 5 minutes to obtain lipid-encapsulated albumin nanoparticles, the obtained lipid albumin nanoparticles are observed by transmission electron microscopy as a regular spherical structure, with a particle size of about 100 nanometers.

Embodiment 3

[0061] Weigh 20 mg of albumin-bound paclitaxel nanoparticles, dissolve with 1 ml of normal saline, add 1 ml of the 20 mg / ml lipid suspension prepared in Example 1, and ultrasonicate at 37 watts for 5 minutes to obtain lipid-encapsulated albumin Conjugated paclitaxel nanoparticles, such as attached figure 1 As shown, the obtained lipid-albumin-bound paclitaxel nanoparticles were observed by transmission electron microscopy as a regular spherical structure with a particle size of about 100 nanometers.

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Abstract

The invention relates to lipid-coated albumin-bound chemotherapeutic drug nanoparticles as well as a preparation method and application thereof. According to the invention, lipid molecules are wrapped on the surfaces of albumin-bound chemotherapeutic drug nanoparticles, and are further mixed with genes to prepare lipid albumin nanoparticles carrying genes and chemotherapeutic drugs; or albumin is prepared into nanoparticles, and lipid molecules are wrapped on the surfaces of the nanoparticles, and are further mixed with the genes to obtain the lipid albumin nanoparticles for cell transfection or gene therapy. The preparation method has the advantages of green and environment-friendly effects, good repeatability, simple and convenient preparation method and high yield, the prepared nanoparticles are regular in morphology and uniform in particle size, the loaded genes have very good transfection efficiency, and the nanoparticles have wide application prospects in the field of tumor treatment, especially the field of drug-resistant tumor treatment.

Description

technical field [0001] The invention relates to the fields of nanoparticle preparation and pharmaceutical preparations, in particular to a lipid-wrapped albumin-binding chemotherapeutic drug nanoparticle and its preparation method and application. Background technique [0002] Chemotherapy combined with surgery is the most common method of cancer treatment, but in chemotherapy, some patients are inherently resistant to chemotherapeutic drugs, while other patients gradually develop tolerance to chemotherapeutic drugs during chemotherapy, tumor chemotherapy drug resistance It is one of the main causes of tumor treatment failure. In addition, during the course of chemotherapy, due to the lack of targeting of chemotherapeutic drugs to tumors, especially after the tumor develops drug resistance, it is necessary to increase the dose of drugs to improve the therapeutic effect, which will cause great side effects on normal tissues and organs of the human body. . Therefore, suppres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/24A61K31/337A61K31/7088A61K47/64A61K47/69A61K48/00A61P35/00B82Y5/00B82Y30/00B82Y40/00
CPCA61K31/337A61K47/643A61K9/5123A61K31/7088A61K47/6929A61K48/0025A61P35/00B82Y5/00B82Y30/00B82Y40/00A61K2300/00
Inventor 许国雄王成龙徐晓琳
Owner JINSHAN HOSPITAL FUDAN UNIV
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